We narrowed to 77,447 results for: rest
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Plasmid#186409PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter Venus-gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only
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pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-HA
Plasmid#186410PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter HA tag under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-SpRYgest_targetplasmid (KAC833)
Plasmid#181748PurposepUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.DepositorTypeEmpty backboneUseSubstrate plasmidAvailable SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
ST*-GFP
Plasmid#163667PurposeExpresses partial length ST in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
TagsGFPExpressionMammalianMutationThis chimera contains the first 113 amino acids o…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-4
Plasmid#163142PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-4
Plasmid#163130PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-3
Plasmid#163141PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-2
Plasmid#163140PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-3
Plasmid#163129PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-5
Plasmid#163131PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-3
Plasmid#160703Purposetef1-alpha promoter from Trichoderma virens bordered by BsaI restriction sitesDepositorInserttef1-alpha
UseSynthetic BiologyMutationIncorporation of BsaI restriction sitesAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CTMP
Plasmid#129560Purposecytoplasmic markerDepositorInsertmCherry
ExpressionBacterialAvailable SinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
SMT215
Plasmid#134818PurposeSMT205 with DHFR-FLAG-tag, western blotDepositorInsertsDihydrofolate reductase
Thymidylate synthase
TagsFLAGExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT101
Plasmid#134815PurposeDual expression of DHFR and TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
ExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7Available SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT201
Plasmid#134816PurposeSMT101 with TET promoter for TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
ExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-D570A-SRDX
Plasmid#129682PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b-SRDX
UseCRISPR; Gateway compatible aaccas12b entry cloneExpressionPlantMutationD570A; AacCas12b is rice codon optimizedAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_AsPT1-LOV2-EcSsra
Plasmid#131217PurposeExpresses AsPT1-LOV2-EcSsra (Avena Sativa) preceded by restriction sitesDepositorInsertAsPT1-LOV2-EcSsra
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_AsPT1-LOV2-pep
Plasmid#131215PurposeExpresses AsPT1-LOV2-pep (Avena sativa) preceded by restriction sitesDepositorInsertAsPT1-LOV2-pep
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL832
Plasmid#119305Purposeprecusor outer membrane protein A, ompA, truncated after amino acid 176DepositorInsertprecursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
TagsnoneExpressionBacterialMutationtruncated after amino acid 176, mutations Threoni…PromoterlacAvailable SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL800
Plasmid#119306Purposeprecusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290SDepositorInsertprecusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
TagsnoneExpressionBacterialMutationamino acids 1 through 171 deleted, and mutatioms …PromoterlacAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only