We narrowed to 3,180 results for: actin

1. p3xFLAG-CMV10-hTti1

   Plasmid

   #30213

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al J Biol Chem. 2010 Jun 25. 285(26):20109-

   Insert

   Tel2 interacting protein 1 (TTI1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pET28a-RILP

   Plasmid

   #74600

   Purpose

   used for expression in E. coli and purification

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   Rab-interacting lysosomal protein (RILP Human)
   

   Use

   Tags

   FLAG, StrepII, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Venus-Bik-DD-pEGFP-C1

   Plasmid

   #177429

   Purpose

   To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.

   Depositor

   David Andrews

   Insert

   Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Threonine 33 and Serine 35 replaced with aspartic…

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. mGarnet2-RITA

   Plasmid

   #104314

   Purpose

   codes for a fusion of fluorescent protein mGarnet2 and RITA (RBP-J Interacting and tubulin-associated), stains microtubular stucturs in live cells

   Depositor

   Ulrich Nienhaus

   Article

   Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

   Insert

   mGarnet2-RITA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Ac5-STABLE2-blast

   Plasmid

   #208034

   Purpose

   Enables expression of 2 ORFs (with or without tags) and selection with blasticidin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-blast with Actin5C promoter.

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Ac5-STABLE1-puro

   Plasmid

   #208031

   Purpose

   Enables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter.

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mGarnet-RITA

   Plasmid

   #104313

   Purpose

   codes for a fusion of fluorescent protein mGarnet and RITA (RBP-J Interacting and tubulin-associated), stains microtubular stucturs in live cells

   Depositor

   Ulrich Nienhaus

   Article

   Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

   Insert

   mGarnet-RITA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEF‐PIK3IP1

   Plasmid

   #49214

   Purpose

   expresses PIK3IP1 in mammalian cells

   Depositor

   Lawrence Kane

   Article

   DeFrances et al Eur J Immunol. 2012 Oct;42(10):2754-9. d

   Insert

   PIK3IP1 (Pik3ip1 Mouse)
   

   Use

   Tags

   6X-his and myc

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pAWH-Rluc-let-7-A114-N40-HhR

   Plasmid

   #50555

   Purpose

   Expression of luciferase reporter mRNA in S2 cell

   Depositor

   Yukihide Tomari

   Article

   Fukaya et al Mol Cell. 2012 Dec 28;48(6):825-36. doi:

   Insert

   Luciferase-let7-A114-N40-HhR
   

   Use

   Luciferase

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCIS.MTRIA-PRLH

    Plasmid

    #184618

    Purpose

    For expression of a fluorescent sensor for Prolactin-releasing peptide factor in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-PRLH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMTB-Multibow-mfR

    Plasmid

    #60991

    Purpose

    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound mKate2.

    Depositor

    Sean Megason

    Article

    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

    Insert

    mKate2
    

    Use

    Zebrafish

    Tags

    membrane tag

    Expression

    Mutation

    Promoter

    Bactin2

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pMTB-Multibow-mG

    Plasmid

    #60987

    Purpose

    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound EGFP

    Depositor

    Sean Megason

    Article

    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

    Insert

    EGFP
    

    Use

    Zebrafish

    Tags

    membrane tag

    Expression

    Mutation

    Promoter

    Bactin2

    Available since

    Jan. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pMTB-Multibow-mR

    Plasmid

    #60990

    Purpose

    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound dTomato

    Depositor

    Sean Megason

    Article

    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

    Insert

    dTomato
    

    Use

    Zebrafish

    Tags

    membrane tag

    Expression

    Mutation

    Promoter

    Bactin2

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pAW-GFP-2xmiR-34 (pAW-AS-25)

    Plasmid

    #40833

    Purpose

    miRNA sensor used in Drosophila cell lines

    Depositor

    Phillip Zamore

    Article

    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

    Insert

    Two target sites perfectly complementary to miR-34
    

    Use

    Mirna reporter

    Tags

    EGFP

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pAWH-Rluc-let-7-Poly(A)

    Plasmid

    #50556

    Purpose

    Expression of luciferase reporter mRNA in S2 cell

    Depositor

    Yukihide Tomari

    Article

    Fukaya et al Mol Cell. 2012 Dec 28;48(6):825-36. doi:

    Insert

    Luciferase-let7-Poly(A)
    

    Use

    Luciferase

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMTB-Multibow-hfR

    Plasmid

    #60998

    Purpose

    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. . Encodes nuclear mKate2.

    Depositor

    Sean Megason

    Article

    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

    Insert

    mKate2
    

    Use

    Zebrafish

    Tags

    histone 2B tag

    Expression

    Mutation

    Promoter

    Bactin2

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pAW-EGFP-2xmiR-1 (pAW-AS-21)

    Plasmid

    #40832

    Purpose

    miRNA sensor used in Drosophila cell lines

    Depositor

    Phillip Zamore

    Article

    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

    Insert

    Two target sites perfectly complementary to miR-1
    

    Use

    Mirna reporter

    Tags

    EGFP

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pMTB-Multibow-mB

    Plasmid

    #60985

    Purpose

    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound EBFP2.

    Depositor

    Sean Megason

    Article

    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

    Insert

    EBFP2
    

    Use

    Zebrafish

    Tags

    membrane tag

    Expression

    Mutation

    Promoter

    Bactin2

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-myc-Rem2 L317G

    Plasmid

    #51586

    Purpose

    expresses RNAiR myc-tagged Rem2 non-CaM interacting

    Depositor

    Suzanne Paradis

    Article

    Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

    Insert

    Rem2 (Rem2 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    L317G; silent mutations at shRNA 1 and 2 recognit…

    Promoter

    CMV

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMTB-Multibow-mO

    Plasmid

    #60989

    Purpose

    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound mKO

    Depositor

    Sean Megason

    Article

    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

    Insert

    mKO
    

    Use

    Zebrafish

    Tags

    membrane tag

    Expression

    Mutation

    Promoter

    Bactin2

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge