161,488 results

1. pTRANS_231

   Plasmid

   #91120

   Purpose

   Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: BeYDV

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSETB/mStayGold(c4)

   Plasmid

   #212018

   Purpose

   For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   mStayGold(c4)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF0113

   Plasmid

   #141520

   Purpose

   Lentiviral vector for overexpressing the SMAD3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SMAD3 (SMAD3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBTK800

   Plasmid

   #179209

   Purpose

   GFP dropout for assembling a double-stranded RNA expression plasmid

   Depositor

   Jeffrey Barrick

   Article

   Lariviere et al Nat Protoc. 2023 Mar;18(3):902-928. doi:

   Insert

   GFP dropout
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pHAGE-UBC-hAAT-IRES-ZsGreen-W

   Plasmid

   #126702

   Purpose

   lentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreen

   Depositor

   Darrell Kotton

   Article

   Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

   Insert

   Alpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   UBC

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCH4 (denAsCas12a-KRAB lenti)

   Plasmid

   #217331

   Purpose

   Lentiviral expression of denAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   denAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Expression

   Mutation

   D908A/E174R/S542R/K548R

   Promoter

   UCOE-SFFV

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Foxf1 pcDNA HisC

   Plasmid

   #122119

   Purpose

   Expression of Foxf1 in mammalian cells

   Depositor

   Paul Herring

   Article

   Hoggatt et al J Biol Chem. 2013 Oct 4;288(40):28477-87

   Insert

   Foxf1 (Foxf1 Mouse)
   

   Use

   Tags

   6xHis and Express

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWN_U6-TRAC-miniGag-Cas9

   Plasmid

   #228959

   Purpose

   miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF0112

   Plasmid

   #141519

   Purpose

   Lentiviral vector for overexpressing the SMAD2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SMAD2 (SMAD2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1+ Flag-RAP2A

    Plasmid

    #128099

    Purpose

    Expresses RAP2A in mammalian cells in a G418-selectable plasmid

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-PEmax-P2A-hMLH1dn

    Plasmid

    #174828

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-hMLH1dn
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript; deletion of residues 754-…

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRVV578

    Plasmid

    #108279

    Purpose

    RMCE vector containing inverted PhiC31 attB sites

    Depositor

    Richard Mann

    Article

    Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

    Insert

    PhiC31 attB sites
    

    Use

    CRISPR; Rmce

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. R989-X02-566: His6-MBP-tev-G-Hs.KRAS4b(1-169)

    Plasmid

    #159539

    Purpose

    E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169)

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-169) (KRAS Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19_GZG

    Plasmid

    #117226

    Purpose

    shble flanked by an endogenous GAPDH promoter and terminator system

    Depositor

    Jackie Collier

    Insert

    shble
    

    Use

    Thraustochytrid expression

    Tags

    Expression

    Mutation

    Promoter

    GAPDH

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBbS8k-RFP

    Plasmid

    #35276

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BAD

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-NeuroD6iresGFP

    Plasmid

    #45027

    Depositor

    Connie Cepko

    Article

    Cherry et al J Neurosci. 2011 May 18;31(20):7365-79.

    Insert

    NeuroD6 (Neurod6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSETb-rsGreen0.7

    Plasmid

    #78176

    Purpose

    Expresses rsGreen0.7 in E. coli strains.

    Depositor

    Peter Dedecker

    Article

    Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

    Insert

    rsGreen0.7
    

    Use

    Tags

    6xHis-tag; Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRM15

    Plasmid

    #163694

    Purpose

    hsp70 promoter inducible expression of DHB:mScarlet-P2A-H2B:miRFP670

    Depositor

    David Matus

    Article

    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

    Insert

    DHB:mScarlet-P2A-H2B:miRFP670 (HELB, H2BC11 Human)
    

    Use

    Zebrafish expression

    Tags

    mScarlet and miRFP670

    Expression

    Mutation

    Promoter

    HSP70

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. SP6-VEE-OKS-iG

    Plasmid

    #58975

    Purpose

    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Inserts

    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    GLIS (GLIS1 Human)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAd-DsRed

    Plasmid

    #17515

    Depositor

    Anton Bennett

    Article

    Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

    Insert

    pAd-DsRed
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2008

    Availability

    Academic Institutions and Nonprofits only