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Showing: 2761 - 2780 of 76964 results

  12. T7-VEE-OKS-iM

    Plasmid
    #58972
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    26S subgenomic promoter and IRES
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58972-plasmid-map-sequence-id-466890

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  14. PB-EEA-5g-OSK2M2L1-PGK-Puro

    Plasmid
    #102909
    Purpose
    PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102909-plasmid-map-sequence-id-200136

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  15. pAG497 mito-iFAST

    Plasmid
    #130819
    Purpose
    Expresses mito-iFAST in mammalian cells (mitochondria labeling)
    Depositor
    Arnaud Gautier
    Insert
    mito-iFAST
    Use
    Tags
    myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    130819-plasmid-map-sequence-id-254957

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  16. SRS-MTBD(19/19), AB(monomer), ux

    Plasmid
    #22389
    Depositor
    Ron Vale
    Insert
    Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
    Use
    Tags
    His and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 24, 2009
    Availability
    Academic Institutions and Nonprofits only
    22389-plasmid-map-sequence-id-10296

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  17. pcDNA-HisFlag-rUSP19ER-CRmut

    Plasmid
    #155253
    Purpose
    for mammalian overexpression of the mutated ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) CR mutant (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Cys(545) to Arg
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155253-plasmid-map-sequence-id-311950

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  18. pcDNA-HisFlag-rUSP19ER deltaN3

    Plasmid
    #155243
    Purpose
    for mammalian overexpression of truncated (delta1-1631) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-1631) (ER isoform) (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155243-plasmid-map-sequence-id-311880

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  19. pcDNA-HisFlag-rUSP19ER deltaN2

    Plasmid
    #155242
    Purpose
    for mammalian overexpression of truncated (delta1-1088) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-1088) (ER isoform) (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155242-plasmid-map-sequence-id-311878

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  20. pcDNA-HisFlag-rUSP19ER deltaN1

    Plasmid
    #155241
    Purpose
    for mammalian overexpression of truncated (delta1-536) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-536) (ER isoform) (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155241-plasmid-map-sequence-id-311860

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Showing: 2761 - 2780 of 76964 results