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Showing: 521 - 560 of 798 results

  10. pAC-94N

    Plasmid
    #53281
    Purpose
    Contains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and produces beta-carotene in E. coli when complemented with a gene encoding geranylgeranyl diphosphate synthase
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    crtY, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Promoter
    endogenous promoters
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53281-plasmid-map-sequence-id-82992

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  12. pAC-ZEAX

    Plasmid
    #53274
    Purpose
    Contains crtE, crtB, crtI, crtY and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces zeaxanthin in E. coli
    Depositor
    Francis X Cunningham Jr
    Article
    Sun et al J Biol Chem. 1996 Oct 4;271(40):24349-52
    Insert
    crtE, crtY, crtI, crtB, crtZ
    Use
    Low copy number bacterial cloning vector
    Mutation
    Deleted idi (ORF6) and crtX genes
    Promoter
    endogenous promoters
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53274-plasmid-map-sequence-id-83289

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  13. pACT5FVMF(f)

    Plasmid
    #110549
    Purpose
    Control plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008
    Depositor
    David Russell
    Article
    Hendrie et al Mol Ther. 2008 Mar;16(3):534-40. doi: 10
    Insert
    EGFP, FV LTRs
    Use
    Qpcr control for titering foamy virus
    Promoter
    promoter: MLV LTR
    Available Since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110549-plasmid-map-sequence-id-212295

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  14. pGDP3 vatD

    Plasmid
    #112885
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    vatD
    Expression
    Bacterial
    Promoter
    Pbla
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112885-plasmid-map-sequence-id-222210

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  15. pGDP1 vph

    Plasmid
    #112893
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    vph
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    Pbla
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112893-plasmid-map-sequence-id-219905

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  16. pGDP3 aph(9)-Ia

    Plasmid
    #112882
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    aph(9)-Ia
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    Pbla
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112882-plasmid-map-sequence-id-219862

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  17. pGDP1 uvrA

    Plasmid
    #112892
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    uvrA-like protein
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    Pbla
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112892-plasmid-map-sequence-id-219901

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  19. pKHR5

    Plasmid
    #74593
    Purpose
    an alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.
    Depositor
    David Grunwald
    Article
    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:
    Insert
    FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
    Use
    Cre/Lox; Zebrafish expression
    Promoter
    alpha-crystallin
    Available Since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    74593-plasmid-map-sequence-id-140738

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  24. pAC-crRNA-Km

    Plasmid
    #158712
    Purpose
    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    AcGFP (for crRNA cloning)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    TetO
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    158712-plasmid-map-sequence-id-321925

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  29. pOGG010

    Plasmid
    #113984
    Purpose
    Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    RK2 (IncP)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113984-plasmid-map-sequence-id-223395

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  30. pAC-ZETA

    Plasmid
    #53316
    Purpose
    Contains Erwinia herbicola Eho10 genes crtE and crtB, together with the pds (crtP) gene of Synechococcus PCC7942 fused to the lacZ gene at the N terminus. Produces zeta-carotene in E. coli.
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    pds
    Use
    Low copy number bacterial cloning vector
    Promoter
    lazZ
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53316-plasmid-map-sequence-id-83035

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  31. pAC-NEUR

    Plasmid
    #53271
    Purpose
    Contains the Erwinia herbicola (Pantoea agglomerans) Eho10 genes crtE and crtB, and the Rhodobacter capsulatus crtI gene and thereby produces neurosporene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    crtI
    Use
    Low copy number bacterial cloning vector
    Promoter
    endogenous promoters
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53271-plasmid-map-sequence-id-82987

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  35. RAB14 3'UTR WT

    Plasmid
    #61489
    Purpose
    luciferase reporter containing RAB14 3' UTR
    Depositor
    Curt Civin
    Article
    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111
    Insert
    RAB14 3' UTR (RAB14 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    contains position 837-1637 (numbering relative to…
    Promoter
    PGK
    Available Since
    Jan. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  37. pJUMP27ts[mCherry]-1A(lacZ)

    Plasmid
    #126980
    Purpose
    Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site.
    Depositor
    Chris French
    Article
    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    RepA A56V thermosensitive mutation
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126980-plasmid-map-sequence-id-248074

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  39. pcDNA3.1-CRISPR-Puro-sgRPL3-56

    Plasmid
    #208979
    Purpose
    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.
    Depositor
    Quanfu Ma
    Insert
    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Synthetic, Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV and U6 in tandem
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208979-plasmid-map-sequence-id-418375

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  40. pcDNA3.1-CRISPR-Puro-sgRPL3-62

    Plasmid
    #208647
    Purpose
    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.
    Depositor
    Quanfu Ma
    Insert
    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Synthetic, Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV and U6 in tandem
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208647-plasmid-map-sequence-id-418374

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Showing: 521 - 560 of 798 results