1. p426 46Q GPD

   Plasmid

   #1182

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 46Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pc DNA FRT TO- APPSwed/Ind

   Plasmid

   #114194

   Purpose

   Creation of a stable cell line expressing APP mutation (AD in vitro study)

   Depositor

   Aleksandra Radenovic

   Insert

   Amyloid-Precursor-Protein (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Popular mouse model, overexpress 2 mutations: Swe…

   Promoter

   CMV

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5/FRT/TO V5 HSPA14

   Plasmid

   #19515

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   heat shock 70kDa protein 14 (HSPA14 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEF-TAZ-N-Flag (S89A)

   Plasmid

   #19026

   Depositor

   Michael Yaffe

   Article

   Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

   Insert

   TAZ (Wwtr1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Vector has no SV40 origin, S89A (site directed mu…

   Promoter

   Available since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

4. p3xFlag-CMV7-Erk2

   Plasmid

   #39223

   Depositor

   Melanie Cobb

   Article

   Robinson et al J Biol Chem. 2002 Apr 26;277(17):14844-5

   Insert

   Erk2 (Mapk1 Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Dead Streptavidin-SpyTag

   Plasmid

   #59548

   Purpose

   pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

   Insert

   Dead Streptavidin-SpyTag
   

   Use

   Tags

   SpyTag

   Expression

   Bacterial

   Mutation

   The construct lacks final K of SpyTag

   Promoter

   T7

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

6. p3xFlag-CMV7-Erk2_KR

   Plasmid

   #39224

   Depositor

   Melanie Cobb

   Article

   Robinson et al J Biol Chem. 2002 Apr 26;277(17):14844-5

   Insert

   Erk2(K52R) (Mapk1 Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K52R

   Promoter

   CMV

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA5/FRT/TO GFP HSPA14

   Plasmid

   #19488

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   HSPA14 (HSPA14 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-P1-N-EGFP-C

   Plasmid

   #159436

   Purpose

   CRISPR knock-in donor construct with fluorescent reporter

   Depositor

   Jianhua Xing

   Article

   Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3' linker pair 1, 5' linker pair 1, C t…

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5/FRT/TO HSPA14

   Plasmid

   #19461

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   HSPA14 (HSPA14 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-P1-N-miRFP670-C

    Plasmid

    #159438

    Purpose

    CRISPR knock-in donor construct with fluorescent reporter

    Depositor

    Jianhua Xing

    Article

    Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

    Insert

    miRFP670
    

    Use

    CRISPR

    Tags

    3' linker pair 1, 5' linker pair 1, C t…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-mTAZ-WW (86)

    Plasmid

    #40915

    Depositor

    Michael Yaffe

    Article

    Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

    Insert

    TAZ (Wwtr1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains mTAZ 14-3-3 binding site and WW domain (…

    Promoter

    Tac

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pGEX-mTAZ-WW (43)

    Plasmid

    #40916

    Depositor

    Michael Yaffe

    Article

    Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

    Insert

    TAZ (Wwtr1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains mTAZ WW domain (43 amino acids, 122-164)

    Promoter

    Tac

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pOPINF HopF2

    Plasmid

    #228397

    Purpose

    Recombinant expression of His6-tagged Pseudomonas syringae pv tomato type III effector HopF2 in E. coli

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    HopF2 (hopF2 Pseudomonas syringae pv. tomato)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR221-HUWE1_R4187C

    Plasmid

    #187141

    Purpose

    Entry clone

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Entry clone

    Tags

    Expression

    Mutation

    R4187C

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221-HUWE1_F3194S

    Plasmid

    #187139

    Purpose

    Entry clone

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Entry clone

    Tags

    Expression

    Mutation

    F3194S

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR221-HUWE1_H669Q

    Plasmid

    #187137

    Purpose

    Entry clone

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Entry clone

    Tags

    Expression

    Mutation

    H669Q

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221-HUWE1_H3962D

    Plasmid

    #187130

    Purpose

    Entry clone

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Entry clone

    Tags

    Expression

    Mutation

    H3962D

    Promoter

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-P1-N-mCherry-C

    Plasmid

    #159437

    Purpose

    CRISPR knock-in donor construct with fluorescent reporter

    Depositor

    Jianhua Xing

    Article

    Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

    Insert

    mCherry
    

    Use

    CRISPR

    Tags

    3' linker pair 1, 5' linker pair 1, C t…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDARMO_HUWE1_3xFLAG

    Plasmid

    #187155

    Purpose

    mammalian cell expression of HUWE1-3xFLAG

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST_FLAG-HUWE1

    Plasmid

    #187120

    Purpose

    mammalian cell expression of FLAG-HUWE1

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge