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We narrowed to 434 results for: T7 rna polymerase

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Showing: 261 - 280 of 434 results

  5. pB2-neo-TET

    Plasmid
    #51287
    Purpose
    expresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Publication
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    sine B2
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Promoter
    enhancer from 7SL gene
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51287-plasmid-map-sequence-id-78369

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  6. pRAV12

    Plasmid
    #67713
    Purpose
    Parental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purification
    Depositor
    Robert Batey, Jeffrey Kieft
    Publication
    Kieft et al RNA. 2004 Jun;10(6):988-95.
    Insert
    Transcription cassette for 3'-tagged RNAs
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    T7 RNA polymerase
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    67713-plasmid-map-sequence-id-126588

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  11. mAC-POLR2A donor (Hygro)

    Plasmid
    #124496
    Purpose
    A donor plasmid for tagging the N-terminus of human POLR2A with mAID-mClover
    Depositor
    Masato Kanemaki
    Publication
    Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    POLR2A homology arms and Hygro-P2A-mAID-mClover (POLR2A Human)
    Use
    CRISPR
    Tags
    mAID-mClover
    Available since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124496-plasmid-map-sequence-id-243723

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  12. SP6-sgRNA-scaffold

    Plasmid
    #47912
    Purpose
    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection
    Depositor
    Paul Sternberg
    Publication
    Chiu et al Genetics. 2013 Aug 26.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    sgRNA 3' end
    Expression
    Worm
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47912-plasmid-map-sequence-id-68534

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  13. SP6-hCas9-Ce-mRNA

    Plasmid
    #47911
    Purpose
    In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.
    Depositor
    Paul Sternberg
    Publication
    Chiu et al Genetics. 2013 Aug 26.
    Insert
    hCas9 with C. elegans 5' and 3' UTRs
    Use
    CRISPR
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47911-plasmid-map-sequence-id-68528

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  14. DENV4 Rluc Reporter GND mutant Replicon

    Plasmid
    #118586
    Purpose
    For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.
    Depositor
    Radhakrishnan Padmanabhan
    Publication
    Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
    Use
    Yeast-e. col shuttle vector prs424
    Mutation
    The conserved GDD in the NS5 polymerase gene muta…
    Available since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    118586-plasmid-map-sequence-id-227283

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  16. C98m

    Plasmid
    #121002
    Purpose
    MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Gp2 toxin
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121002-plasmid-map-sequence-id-236135

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Showing: 261 - 280 of 434 results