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Showing: 5561 - 5600 of 13635 results

  3. pWPT-/UAA-mEGFP-IRES-mCherry

    Plasmid
    #49234
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49234-plasmid-map-sequence-id-73657

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  7. pKK-RNAi-nucCHERRYmiR-TEV-EGFP

    Plasmid
    #105814
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal EGFP.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105814-plasmid-map-sequence-id-203426

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  9. KY801: pMVP (L3-L2) P2A-eGFP + polyA

    Plasmid
    #121764
    Purpose
    pMVP L3-L2 entry plasmid, contains eGFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-eGFP + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121764-plasmid-map-sequence-id-237247

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  13. pcDNA4/TO-bio-myc-NES-EGFP

    Plasmid
    #112712
    Purpose
    For tetracycline-inducible expression of bio-myc-NES-EGFP in mammalian cells
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Insert
    EGFP
    Use
    Tags
    HIV Rev nuclear localization signal (NES), biotin…
    Expression
    Mammalian
    Mutation
    Nucleotide sequence optimized for synthetic gene …
    Promoter
    CMV with Tet repressor binding sites
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    112712-plasmid-map-sequence-id-217058

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  28. pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT

    Plasmid
    #238281
    Purpose
    For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT
    Depositor
    Denes Hnisz
    Insert
    mCherry-P2AT2A-EGFP-BRD4-NUT
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    238281-plasmid-map-sequence-id-474571

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  29. mscv_EGFP-IRES-NUP98-KDM5A-KS-FtoG

    Plasmid
    #238292
    Purpose
    For overexpression of EGFP-IRES-NUP98-KDM5A-KS-FtoG
    Depositor
    Denes Hnisz
    Insert
    EGFP-IRES-NUP98-KDM5A-KS-FtoG
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    238292-plasmid-map-sequence-id-474560

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  30. pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT-KS

    Plasmid
    #238282
    Purpose
    For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT-KS
    Depositor
    Denes Hnisz
    Insert
    mCherry-P2AT2A-EGFP-BRD4-NUT-KS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238282-plasmid-map-sequence-id-474428

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  31. mscv_EGFP-IRES-NUP98-KDM5A-KS-FtoA

    Plasmid
    #238291
    Purpose
    For overexpression of EGFP-IRES-NUP98-KDM5A-KS-FtoA
    Depositor
    Denes Hnisz
    Insert
    EGFP-IRES-NUP98-KDM5A-KS-FtoA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238291-plasmid-map-sequence-id-474364

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  32. pMRX-IP-EGFP-PA-BAX(mito, G108V)

    Plasmid
    #233343
    Purpose
    Stable expression of EGFP-LOV2(N538E)-BAX(G108V)-OMP25 in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    Omp25 (Synj2bp Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)…
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233343-plasmid-map-sequence-id-469412

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  33. pMRX-IP-EGFP-PA-BAX(lyso, G108V)

    Plasmid
    #233345
    Purpose
    Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    TMEM106B (TMEM106B Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    BAX(3-171), N-terminus and C-terminus are deleted…
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233345-plasmid-map-sequence-id-469414

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  34. pAG Lenti CMV HA-EGFP Puro

    Plasmid
    #236081
    Purpose
    Lentiviral backbone expressing N-terminally HA tagged EGFP, control for empty backbone 236079
    Depositor
    Melina Fan
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236081-plasmid-map-sequence-id-467821

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  35. pAG Lenti CMV EGFP-HA Puro

    Plasmid
    #236082
    Purpose
    Lentiviral backbone expressing C-terminally HA tagged EGFP, control for empty backbone 236080
    Depositor
    Melina Fan
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236082-plasmid-map-sequence-id-467819

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  36. pAG Lenti CMV EGFP Puro

    Plasmid
    #236084
    Purpose
    Lentiviral backbone expressing EGFP, control for empty backbone 236083
    Depositor
    Melina Fan
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236084-plasmid-map-sequence-id-467824

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  37. pAG426-GAL-SoxN-A22-EGFP

    Plasmid
    #181731
    Purpose
    Galactose inducible expression of SoxN-A22-EGFP
    Depositor
    Alejandro Chavez
    Insert
    SoxN-A22-EGFP (SoxN Fly)
    Use
    Tags
    3xFLAG-EGFP
    Expression
    Yeast
    Mutation
    extended repeat of 22 Ala residues replacing nati…
    Promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    181731-plasmid-map-sequence-id-468613

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  39. pCMV-T7-PEmax-P2A-EGFP (LM1589)

    Plasmid
    #223136
    Purpose
    pCMV and pT7 Human expression plasmid for PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized PEmax-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and NLS(SV40)-NLS(cMyc)-P2A-EGFP
    Expression
    Mammalian
    Mutation
    M-MLV mutations from PE2 (Anzalone et al. Nature …
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223136-plasmid-map-sequence-id-447644

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Showing: 5561 - 5600 of 13635 results