We narrowed to 8,237 results for: FIE
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Plasmid#179202PurposeA block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6∆E3-GFP and pAd5-B7.DepositorInsertModified block 6/7, with deletion in E3 and insertion of GFP cassette
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKCyR8
Plasmid#149494PurposeBroad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Hygromycin Resistance Marker.DepositorInsertCymR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCymRCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestRFP_RNAseH1
Plasmid#65785Purposeexpresses RFP-tagged RNAseH1 in mammalian cellsDepositorInsertRNaseH1 (RNASEH1 Zebrafish, Human)
UseTagsRFP and V5ExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET_RTX_(exo-)
Plasmid#102786PurposeExpresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)DepositorInsertreverse transcription xenopolymerase with 3'-5' deficient exonuclease
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET_RTX
Plasmid#102787PurposeExpresses the Reverse Transcriptase geneDepositorInsertreverse transcription xenopolymerase
UseTagsExpressionBacterialMutationPromoterT7 promoter + lac operatorAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWLxR5
Plasmid#149490PurposeBroad-Host range expression vector, incW (pSa) origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
FIRES-PBCS-CLV3
Plasmid#228697PurposeSensor cassette FIRE1, FIRE fluorescent ratiometric sensorDepositorInsertFIRE1 SENSOR
UseSynthetic Biology and UnspecifiedTagsmClover3ExpressionMammalianMutationPromoterhpgkAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
FIREC-PBCS-MC2
Plasmid#228698PurposeControl cassette FIRE1, FIRE fluorescent ratiometric sensorDepositorInsertFIRE1 CONTROL
UseSynthetic Biology and UnspecifiedTagsmCherryExpressionMammalianMutationPromoterEF1-alphaAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B1-deltaE1-GFP
Plasmid#179203PurposeBlock 1 with deletion in E1 and insertion of GFP expression cassetteDepositorInsertModified Block 1 with deletion in E1 and insertion of GFP expression cassette
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pComb3HSS
Plasmid#64133Purpose2nd generation plasmid for phage display on modified geneIII, contains stuffer fragmentDepositorInsertsSS Stuffer
Light Chain Stuffer
Heavy Chain Stuffer
UsePhage displayTagsgeneIIIExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
TRMPVIR
Plasmid#27994DepositorInsertTRMPVIR (shRen)
UseRNAi and Retroviral; Fluorescent reportersTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRXTN
Plasmid#47916PurposepRXTN is a modified version of pRX-tight Puro (part of the two plasmid Tet-ON system from Clontech) encoding NAT instead of PAC.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterTetAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
FIRES-PBCS-E670
Plasmid#228699PurposeSensor cassette FIRE2, FIRE fluorescent ratiometric sensorDepositorInsertFIRE2 SENSOR
UseSynthetic Biology and UnspecifiedTagsExpressionMammalianMutationPromoterhpgkAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
FIREC-PBCS-MR3
Plasmid#228700PurposeControl cassette FIRE2, FIRE fluorescent ratiometric sensorDepositorInsertFIRE2 CONTROL
UseSynthetic Biology and UnspecifiedTagsExpressionMammalianMutationPromoterEF1-alphaAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFNR5
Plasmid#149483PurposeBroad-Host range expression vector, RSF1010 origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertNahR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPSalTTCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion eGFP
Plasmid#112848PurposeGeneral donor vector with EGFP coding sequence flanking by restriction sites for generating knock-in donorDepositorInsertEGFP
UseUnspecified; General donorTagsExpressionMutationPromoterNoAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSKrnaSeph
Plasmid#67506PurposeExpress the tRNALys scaffold with the sephadex aptamer tagDepositorInsertmodified tRNALys with sephadex aptamer
UseTagsExpressionBacterialMutationPromoterlppAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBSMrnaSeph
Plasmid#67507PurposeExpress the tRNAMet scaffold with the sephadex aptamer tagDepositorInsertmodified tRNAMet with sephadex aptamer
UseTagsExpressionBacterialMutationPromoterlppAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLxR5
Plasmid#149482PurposeBroad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLlR5
Plasmid#149464PurposeBroad-Host range expression vector, RK2 and pUC origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlacO-1Available sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTG2
Plasmid#149475PurposeBroad-Host range expression vector, pBBR origin, TetR repressor, PTetA promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertTetR-GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTetAAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-KRAB(A)
Plasmid#72639PurposeTol2 based UAS modified with a IL2RGTC binding site upstream of it, driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and KRAB(A) repressor domainDepositorInsertUAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)
UseGatewayTagsExpressionMutationStop codon after mCherry- SEE DEPOSITOR COMMENTS …PromoterAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNCS
Plasmid#17362DepositorInsertMMLV proviral DNA
UseTagsExpressionMutationPromoterAvailable sinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_HuC
Plasmid#65756Purposeexpresses FLAGHA-tagged HuC in mammalian cellsDepositorInsertELAVL3 (ELAVL3 Human)
UseTagsFLAG HA and V5ExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestGFP_HuC
Plasmid#65762Purposeexpresses GFP-tagged HuC in mammalian cellsDepositorInsertELAVL3 (ELAVL3 Human)
UseTagsFLAG HA and V5ExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBNG2
Plasmid#149473PurposeBroad-Host range expression vector, pBBR origin, NahR(AM) repressor, PSalTTC promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertNahR(AM)-GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPSalTTCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-NLStdMCP- tagRFP
Plasmid#86244PurposeMiddle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP-tagRFPDepositorTypeEmpty backboneUseUnspecifiedTagsNLS-tdMCP-tagRFPExpressionMutationPromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD2rsE_RS1
Plasmid#219723PurposeDV2 resurfaced E dimer to display 2D22/EDE regionDepositorInsertDV2 sE RS1
UseTags8xHis and human serum albumin (HSA)ExpressionMammalianMutationK51R, Q52N, A54Q, K64E, T76D, N83S, L107K, G106D,…PromoterAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLIGFP_W220F
Plasmid#46772PurposeProtein overexpression with the modified LacIWF repressorDepositorInsertLacI_W220F (lacI )
UseTagsExpressionMutationTryptophan 220 replaced by PhenylalaninePromoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLPC-C-SF-TAP-DEST-TLK1-WT
Plasmid#218829PurposeExpresses C-terminal Strep-FLAG-tagged wild type human TLK1 variant 5 in a retrovirus vector.DepositorInsertTLK1 (TLK1 Human)
UseTagsFLAG-STREPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFAR5
Plasmid#149478PurposeBroad-Host range expression vector, RSF1010 origin, AraC activator/repressor, PBAD promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertAraC-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKCiR5
Plasmid#149462PurposeBroad-Host range expression vector, RK2 and pUC origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertCinR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCinAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBCiG2
Plasmid#149470PurposeBroad-Host range expression vector, pBBR origin, CinR(AM) activator, PCin promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertCinR(AM)-GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCinAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ111
Plasmid#175052PurposerbcLXS7002 integrative plasmid expresses CRE recombinase as a translational fusion to rbcLDepositorInsertCRE
UseUnspecifiedTagsExpressionMutationPromoterrbcLXSAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKNR5
Plasmid#149466PurposeBroad-Host range expression vector, RK2 and pUC origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertNahR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPSalTTCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZD-CJ1111
Plasmid#175058PurposerbcLXS6803 integrative plasmid expresses CRE recombinase as a translational fusion to rbcL6803DepositorInsertCRE
UseUnspecifiedTagsExpressionMutationPromoterrbcLXSAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330-Puro-ccdB
Plasmid#82580PurposeA modified version of plasmid pX330 (#42230): Added are a ccdB stuffer and a Puromycin selection cassetteDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCBhAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLxR5
Plasmid#149465PurposeBroad-Host range expression vector, RK2 and pUC origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DestFLAGHA_HuC
Plasmid#65759Purposeconstitutively expresses FLAG-HA-tagged HuC in mammalian cellsDepositorInsertELAVL3 (ELAVL3 Human)
UseTagsFLAG HAExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS_24x-601_MA1+MA2
Plasmid#114361Purpose24x Widom 601 nucleosome sequenceDepositorInsert24X601-MA1_2
UseUnspecifiedTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only