We narrowed to 6,775 results for: /1000

1. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

   Plasmid

   #53225

   Purpose

   human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis

   Depositor

   Jesse Rinehart

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Phosphoprotein synthesis

   Tags

   HIS and MBP

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli, both codons for Ser21…

   Promoter

   pLtetO

   Available since

   May 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-neo VPS50-13Myc

   Plasmid

   #175093

   Purpose

   13Myc tagged VPS50 is expressed in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Schindler et al Nat Cell Biol. 2015 May;17(5):639-50. do

   Insert

   VPS50 (CCDC132 Human)
   

   Use

   Tags

   13Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 145-pGL3ctrl-3' UTR

   Plasmid

   #28175

   Depositor

   Michael Kastan

   Article

   Chen et al Genes Dev. 2010 Oct 1. 24(19):2146-56.

   Insert

   Luciferase flanked by tp53 UTRs (TP53 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Note that insert length is based on sum of the 2 …

   Promoter

   Available since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pLX302-JSP1-V5 puro

   Plasmid

   #87773

   Purpose

   Expresses V5 tagged human JSP1 (DUSP22)

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   JSP1 (DUSP22 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGex-6p2_FANCM

   Plasmid

   #221379

   Purpose

   Expresses FANCM in bacterial cells

   Depositor

   Albert Jeltsch

   Article

   Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

   Insert

   Fanconi anemia group M protein (FANCM Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBluescript GPX-1

   Plasmid

   #13038

   Depositor

   Peter Newburger

   Article

   Shen et al J Biol Chem. 1993 May 25. 268(15):11463-

   Insert

   GPX-1 (GPX1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Additional GCG upstream of codon 11. Codon 92 is …

   Promoter

   Available since

   Nov. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pGEX-p53 R175H

   Plasmid

   #39480

   Depositor

   Ie-Ming Shih

   Article

   Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

   Insert

   TP53 (TP53 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   P72R and R175H

   Promoter

   Available since

   Sept. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pEMI91

   Plasmid

   #74888

   Purpose

   Shuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more information

   Depositor

   Piotr Marszalek

   Article

   Scholl et al Biomacromolecules. 2016 Jun 8.

   Insert

   Titin I27 x 9 (TTN Human)
   

   Use

   Tags

   His-tag and Strep-tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. PA-mCherry1-Vimentin-7

   Plasmid

   #57202

   Purpose

   Localization: Intermediate Filaments, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Article

   York et al Nat Methods. 2011 Apr;8(4):327-33. doi:

   Insert

   Vimentin (VIM Human)
   

   Use

   Tags

   PA-mCherry1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. ATG9A-2STrep

    Plasmid

    #210359

    Purpose

    Expresses ATG9A fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    ATG9A (ATG9A Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. GBSGFP insulator

    Plasmid

    #121975

    Purpose

    Gli activity reporter, mouse transgenesis

    Depositor

    James Briscoe

    Article

    Balaskas et al Cell. 2012 Jan 20;148(1-2):273-84. doi:

    Insert

    GBS(long)-GFP-insulator (Gnb5 Mouse)
    

    Use

    Mouse transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

12. pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S

    Plasmid

    #62459

    Purpose

    expresses his-tagged C288S human Keap1 protein

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    His-Halo-Tev

    Expression

    Mammalian

    Mutation

    Changed Cysteine 288 of Keap1 to serine

    Promoter

    CMV

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. mTagBFP2-Integrin-Beta1-N-18

    Plasmid

    #55303

    Purpose

    Localization: Integrin/Focal Adhesions, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    Integrin-Beta1 (ITGB1 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. NHA-hnRNPK

    Plasmid

    #120389

    Purpose

    Expression of HA tagged hnRNPK-lambdaN fusion

    Depositor

    Igor Ulitsky

    Article

    Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

    Insert

    hnRNPK (HNRNPK Human)
    

    Use

    Tags

    HA and LambdaN

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHCMV_VSVgSS-diCR2

    Plasmid

    #207324

    Purpose

    Expression of secreted diCR2-10his competitor protein which can inhibit VSV-G mediated infection

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    diCR2 (LDLR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fragment of LDL-R dimerized

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. RnBarr1-mVenus

    Plasmid

    #137790

    Purpose

    Visualization of beta-arrestin1

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin 1 (Arrb1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-HA2-Keap1 S3H4

    Plasmid

    #21594

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    kelch-like ECH-associated protein 1 (KEAP1 Human)
    

    Use

    Tags

    HA2

    Expression

    Mammalian

    Mutation

    Mutated residues in the beta-sheet S3 and alpha-h…

    Promoter

    Available since

    July 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. Actin binding domain of Ezrin

    Plasmid

    #62381

    Purpose

    Expresses the actin binding domain of ezrin. Amino acids 552-586.

    Depositor

    Guillaume Charras

    Article

    Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

    Insert

    Actin binding domain of ezrin (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains aa's 552-586

    Promoter

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmIL-6 mut C/EBP

    Plasmid

    #61291

    Purpose

    drives luciferase from mouse IL-6 promoter with mutant C/EBP (NF-IL-6) site

    Depositor

    Gail Bishop

    Article

    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

    Insert

    IL-6 promoter (Il6 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Mutated C/EBP binding site from ACATTGTGCAATCT to…

    Promoter

    IL-6 promoter with mutant C/EBP binding site

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

20. Beta1-GFP pFB-Neo

    Plasmid

    #69767

    Purpose

    Expresses Beta1-GFP integrin for retroviral transfection

    Depositor

    Martin Humphries

    Article

    Parsons et al J Cell Sci. 2008 Feb 1;121(Pt 3):265-71.

    Insert

    Beta1 integrin (ITGB1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    IRES

    Available since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only