12,254 results

1. pPTK002-2-pGAP

   Plasmid

   #84961

   Purpose

   Type 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoter

   Depositor

   Volker Sieber

   Article

   Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

   Insert

   Glyceraldehyde-3-phosphate dehydrogenase promoter
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   BsaI site removed (1805 c to g)

   Promoter

   Non

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH04_32_sRNA-A_Sp_coD

   Plasmid

   #120900

   Purpose

   the component plasmid of a regulated device

   Depositor

   Domitilla Del Vecchio

   Article

   Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

   Inserts

   pECF32 promoter [i.e., Pecf32_1122 (-60 to -1)]
   sRNA-A
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. TB:7xSMAD_RE:MiniP

   Plasmid

   #133879

   Purpose

   7 copies the SMAD DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   7 copies the SMAD DNA binding motif
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Mammalian

   Available Since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2xp53_RE

   Plasmid

   #118053

   Purpose

   GBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   2 copies of the p53 DNA binding motif
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Triple ORF LoxP ifgMosaic Donor (LH208)

   Plasmid

   #99625

   Purpose

   To clone the 3 ORFs for Cre dependent generation of ifgMosaics

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   N-PhiM
   

   Use

   Cre/Lox, Mouse Targeting, and Synthetic Biology

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-T7-Bottom-T

   Plasmid

   #87310

   Purpose

   Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the T7 promoter.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli system "Bottom" transcript
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-nEF-Con/Foff 2.0-bREACHes-EYFP

   Plasmid

   #137146

   Purpose

   Intersectional viral expression of bREACHes-EYFP in cells expressing Cre AND NOT Flp

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Insert

   Con/Foff 2.0-bREACHes-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUBQ10::ZF108_3xFLAG_YPet

   Plasmid

   #106441

   Purpose

   pUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter in arabidopsis

   Depositor

   Steven Jacobsen

   Article

   Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

   Insert

   ZF108_3xFLAG_YPet
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHflu2

   Plasmid

   #203880

   Purpose

   pHI1 with insertion of ampicillin-resistance gene

   Depositor

   Bogumil Karas

   Insert

   broad-spectrum class A beta-lactamase TEM-116
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL0_19 [panARS ORI]

    Plasmid

    #198932

    Purpose

    Level 0 part

    Depositor

    Cultivarium Tools, Nili Ostrov, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    panARS ORI
    

    Use

    Synthetic Biology

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDYSCKO-ADE1

    Plasmid

    #120264

    Purpose

    pDYSCKO canavanine co-selection vector with a guide targeting the ADE1 locus

    Depositor

    Christian Landry

    Article

    Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

    Insert

    pDYSCKO cassette-ADE1 targeting guide
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    SNR52

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAM17

    Plasmid

    #86473

    Purpose

    Expresses p12 and p9

    Depositor

    Pamela Silver

    Article

    Myhrvold et al ACS Synth Biol. 2016 Aug 3.

    Insert

    p12, p9
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPTK005-3a-αMF

    Plasmid

    #84964

    Purpose

    Type 3a, α-mating factor - secretion signal

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    α-mating factor secretion tag
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Non

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV plasmid

    Plasmid

    #169739

    Purpose

    Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUdOm1.0

    Plasmid

    #210298

    Purpose

    Empty backbone with ampicillin resistance for high expression in mammalian cells with an added 5'UTR

    Depositor

    Corrie daCosta

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUdOm1.2

    Plasmid

    #210274

    Purpose

    Empty backbone with ampicillin resistance for high expression in mammalian cells

    Depositor

    Corrie daCosta

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUdOm1.3

    Plasmid

    #210275

    Purpose

    Empty backbone with ampicillin resistance for intermediate expression in mammalian cells

    Depositor

    Corrie daCosta

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUdOm1.4

    Plasmid

    #210276

    Purpose

    Empty backbone with ampicillin resistance for low expression in mammalian cells

    Depositor

    Corrie daCosta

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUdOm1.5

    Plasmid

    #210277

    Purpose

    Empty backbone with ampicillin resistance for low expression in mammalian cells

    Depositor

    Corrie daCosta

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHH29_32 series plasmid

    Plasmid

    #120901

    Purpose

    Also known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated device

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

    Inserts

    Ec-TTL-P109
    sRNA-A targeting sequence
    RBS TIR=974 of GFPop1 gene
    GFPop1
    RBS TIR=6474 of ECF32mut gene
    ECF32mut
    T7 terminator
    resource competitor
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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