We narrowed to 9,659 results for: SUB;

1. pUC18-GFP

   Plasmid

   #220495

   Purpose

   To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.

   Depositor

   Sébastien Britton

   Article

   Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

   Insert

   EGFP
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   July 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. Flag-HA-PSMD14

   Plasmid

   #22557

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   PSMD14 (PSMD14 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE zeo PPP2CA Wt

   Plasmid

   #10689

   Depositor

   William Hahn

   Article

   Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

   Insert

   protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHen2 Avi-tag-linker-p3

   Plasmid

   #119819

   Purpose

   For cloning and subsequent expression of peptide fragments fused with Avi-tag (for biotinylation) and with pIII protein of M13K07 bacteriophage

   Depositor

   Alexander Gabibov

   Article

   Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

   Insert

   linker with Avi-tag and p3
   

   Tags

   Avi-tag

   Expression

   Bacterial

   Available Since

   May 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEVA3b61

   Plasmid

   #58310

   Purpose

   Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.

   Depositor

   Tom Ellis

   Article

   Wright et al ACS Synth Biol. 2014 May 13.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR::rpsL

   Plasmid

   #44505

   Purpose

   A crRNA expression plasmid specific to the rpsL allele.

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   CRISPR::rpsL (rpsL )
   

   Use

   CRISPR; E.coli

   Available Since

   April 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMIG-PP2A-Abeta

   Plasmid

   #15248

   Depositor

   William Hahn

   Article

   Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

   Insert

   PP2A regulatory subunit A, beta isoform (PPP2R1B Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

8. Flag-HA-PSMD7

   Plasmid

   #22558

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   PSMD7 (PSMD7 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. p18S.1 wt

   Plasmid

   #51727

   Purpose

   transcribes wild type 18S without modifications

   Depositor

   Vincent Mauro

   Article

   Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

   Insert

   18S
   

   Promoter

   pol-I

   Available Since

   July 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. PKC delta dNPS

    Plasmid

    #16387

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC delta (Prkcd Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal pseudosubstrate domain deletion: aa 15…

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pIV101

    Plasmid

    #196671

    Purpose

    Enables recombination mediated gene delivery into Agrobacterium strains for subsequent transformation of plants

    Depositor

    Simona Radutoiu

    Article

    Ferguson et al PLoS One. 2023 Nov 1;18(11):e0291680. do

    Type

    Empty backbone

    Use

    Integration vector

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p18S.2 tag

    Plasmid

    #51731

    Purpose

    transcribes truncated transcript containing 18S and hybridization tag

    Depositor

    Vincent Mauro

    Article

    Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

    Insert

    18S
    

    Available Since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGGA004

    Plasmid

    #48815

    Purpose

    Provides the 35S promoter as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    35S promoter
    

    Use

    Golden gate compatible cloning vector

    Mutation

    internal BsaI recognition site removed by substit…

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBluescript-Evf1

    Plasmid

    #99479

    Purpose

    Subcloning of the Evf1 long non-coding RNA

    Depositor

    Jhumku Kohtz

    Article

    Feng et al Genes Dev. 2006 Jun 1;20(11):1470-84. Ep

    Insert

    Evf1
    

    Expression

    Bacterial

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KW1

    Plasmid

    #21211

    Depositor

    Paul Khavari

    Article

    Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

    Insert

    Mek2 (MAP2K2 Human)
    

    Use

    Subclone vector

    Mutation

    KW71, constitutive active

    Available Since

    July 24, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pCZGY2845

    Plasmid

    #87763

    Purpose

    Pnmr-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in subset of interneurons.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Pnmr-1-PH-miniSOG
    

    Tags

    PH

    Expression

    Worm

    Promoter

    Pnmr-1

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. W1

    Plasmid

    #21208

    Depositor

    Paul Khavari

    Article

    Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

    Insert

    Mek1 (MAP2K1 Human)
    

    Use

    Subclone vector

    Available Since

    July 24, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pWS1037

    Plasmid

    #123049

    Purpose

    G_ subunit

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    Gpa1 [3-4a]
    

    Expression

    Yeast

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCI-neo-μ2-(HA)3

    Plasmid

    #198178

    Purpose

    Expression of HA-tagged AP-2 μ2 in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

    Insert

    AP-2 μ2
    

    Tags

    triple HA tag

    Expression

    Mammalian

    Mutation

    silent substitution in codon 80

    Promoter

    CMV

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1hisA cdc25c S216A

    Plasmid

    #10968

    Depositor

    Toren Finkel

    Article

    Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

    Insert

    cdc25c S216A (CDC25C Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S216A. Serine to Alanine substitution at aa216.

    Available Since

    Nov. 23, 2005

    Availability

    Academic Institutions and Nonprofits only

21. GST cdc25c C330S C377S

    Plasmid

    #10970

    Depositor

    Toren Finkel

    Article

    Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

    Insert

    cdc25c C330S C377S (CDC25C Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C330S C377S. Cysteine to Serine substitution at …

    Available Since

    Nov. 23, 2005

    Availability

    Academic Institutions and Nonprofits only

22. p40 FL

    Plasmid

    #19013

    Depositor

    Michael Yaffe

    Article

    Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

    Insert

    p40phox (NCF4 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

23. wt Cus2p

    Plasmid

    #73046

    Purpose

    Wild-type S. cerevisiae Cus2p gene for recombinant expression and crystallization

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 Dec 2.

    Insert

    CUS2p (CUS2 Budding Yeast)
    

    Tags

    6x His tag fused to TEV cleavage site

    Expression

    Bacterial and Yeast

    Promoter

    T7

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA3.1hisA cdc25c C330S

    Plasmid

    #10966

    Depositor

    Toren Finkel

    Article

    Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

    Insert

    cdc25c C330S (CDC25C Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    C330S. Cysteine to Serine substitution at aa330.

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

25. pTYB1-T4gp46

    Plasmid

    #228211

    Purpose

    Expression in BL21(DE3) of T4 SbcC-like subunit of palindrome specific endonuclease

    Depositor

    Stephen Benkovic

    Article

    Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

    Insert

    46
    

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. NFfshb-t2a-GFP

    Plasmid

    #194356

    Purpose

    over expression vector for killifish fshb tagged with GFP

    Depositor

    Itamar Harel

    Article

    Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

    Insert

    follicle stimulating hormone beta subunit
    

    Use

    Lentiviral

    Tags

    t2a-GFP

    Expression

    Mammalian

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNC-RFP-N

    Plasmid

    #193412

    Purpose

    Nimble cloning-compatible vector for protein subcellular localization in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pNC-RFP-C

    Plasmid

    #193413

    Purpose

    Nimble cloning-compatible vector for protein subcellular localization in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNC-mCherry-N

    Plasmid

    #193414

    Purpose

    Nimble cloning-compatible vector for protein subcellular localization in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNC-mCherry-C

    Plasmid

    #193415

    Purpose

    Nimble cloning-compatible vector for protein subcellular localization in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pNC-CFP-N

    Plasmid

    #193410

    Purpose

    Nimble cloning-compatible vector for protein subcellular localization in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pNC-CFP-C

    Plasmid

    #193411

    Purpose

    Nimble cloning-compatible vector for protein subcellular localization in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pNC-Amp-GFP-C

    Plasmid

    #193417

    Purpose

    Nimble cloning-compatible vector for protein subcellular localization in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pNC-Amp-GFP-N

    Plasmid

    #193416

    Purpose

    Nimble cloning-compatible vector for protein subcellular localization in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCS2+-dCas9-olEzh2(deltaSET)

    Plasmid

    #180217

    Purpose

    Ezh2 (delta SET domain) fused to dCas9 for H3K27me3 epigenome editing control.

    Depositor

    Hiroyuki Takeda

    Article

    Fukushima et al Epigenetics Chromatin. 2019 Mar 13;12(1)

    Insert

    enhancer of zeste 2 polycomb repressive complex 2 subunit (deltaSET)
    

    Tags

    FLAG and dCas9

    Expression

    Bacterial

    Available Since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pQLinkHD_LSSmOrange

    Plasmid

    #118860

    Purpose

    fluorescent protein expression with an N-terminal His-tag

    Depositor

    Marc Birtwistle

    Article

    Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

    Insert

    LSSmOrange
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    A44V,F84L, W145M, I165D, M167L, G202D substituti…

    Available Since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MPS-1-cmyc

    Plasmid

    #113059

    Purpose

    Expresses worm Myc-tagged MPS-1 in mammalian cells

    Depositor

    Federico Sesti

    Article

    Wang et al Biophys J. 2007 Nov 1;93(9):3083-91. doi

    Insert

    MiRP K+ channel accessory Subunit 1 (mps-1 Nematode)
    

    Tags

    c-Myc

    Expression

    Mammalian

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. KHT-1

    Plasmid

    #113054

    Purpose

    Expresses worm KHT-1 in mammalian cells

    Depositor

    Federico Sesti

    Article

    Cai et al EMBO J. 2009 Jun 3;28(11):1601-11. doi:

    Insert

    K+ channel for Habituation to Tap subunit 1
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMVmyc-ORF73SOCS

    Plasmid

    #21546

    Depositor

    J Simas

    Article

    Rodrigues et al EMBO J. 2009 May 6. 28(9):1283-95.

    Insert

    ORF73 (ORF73 MuHV-4)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    amino acids at positions 199, 202, 203 and 206 we…

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

40. pSNAPf-Cox8A Control Plasmid

    Plasmid

    #101129

    Purpose

    Expression of SNAP-Tagged Cox8A in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    Cox8A (COX8A Human)
    

    Tags

    SNAP-tag (SNAPf)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pRSF-1b-S2

    Plasmid

    #128590

    Purpose

    Expresses tagless S2 ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S2 (rpsB E.coli)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET21a-Streptavidin-Alive

    Plasmid

    #20860

    Purpose

    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Alive
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Wt gene

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET21a-Streptavidin-Dead

    Plasmid

    #20859

    Purpose

    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Dead
    

    Expression

    Bacterial

    Mutation

    Triple mutant of wt Streptavidin N23A,S27D,S45A

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pRS405-FlagPEN2/PS1wt

    Plasmid

    #207803

    Purpose

    Yeast integrative vector for expression of FlagPEN2 and Presenilin 1

    Depositor

    Dieter Langosch

    Article

    Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

    Insert

    Gamma-secretase subunits PEN-2 and presenilin1 wt
    

    Expression

    Yeast

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pRS402-nicastrin/APH-1a-HA

    Plasmid

    #207804

    Purpose

    Yeast integrative vector for expression of Nicastrin and APH-1a-HA

    Depositor

    Dieter Langosch

    Article

    Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

    Insert

    Gamma-secretase subunits nicastrin and Aph1a
    

    Expression

    Yeast

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pL-rpb1-N488D

    Plasmid

    #10988

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-N488D (RPO21 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 1462 A to G in the RPB1 ORF resulting in…

    Available Since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

47. pCMV-PEmax-SpG

    Plasmid

    #211549

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

    Insert

    PEmax-SpG
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pRL623

    Plasmid

    #58494

    Purpose

    helper plasmid for bacterial conjugal DNA transfer

    Depositor

    Peter Wolk

    Article

    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

    Insert

    encodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    

    Available Since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. mCherry-Sec61β

    Plasmid

    #172445

    Purpose

    Expression of ER protein Sec61beta with an N-terminal mCherry tag

    Depositor

    Stephen Royle

    Article

    Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Protein transport protein Sec61 subunit beta
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pLEXSY-hyg2.1_LtIFT43_Flag

    Plasmid

    #194433

    Purpose

    Expression of a flag tagged version of L. tarentolae intraflagellar transport protein-43 (IFT43) in Leishmania tarentolae

    Depositor

    Alan Brown

    Article

    Meleppattu et al Cell. 2022 Dec 22;185(26):4986-4998.e12.

    Insert

    Intraflagellar transport complex subunit 43
    

    Use

    Leishmania tarentolae expression

    Tags

    3X Flag and Linker

    Promoter

    L.donovani promoter elements

    Available Since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge