We narrowed to 17,819 results for: Mut

1. EGFR D770-N771 insNPG

   Plasmid

   #11016

   Purpose

   Retroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR D770-N771 insNPG (EGFR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   D770-N771 insNPG

   Promoter

   Available Since

   Dec. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

2. EGFR (del3) L747-E749del, A750P

   Plasmid

   #11015

   Purpose

   Retroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR (del3) (EGFR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   L747-E749 deleted, A750P

   Promoter

   Available Since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA6 asyn A30P

   Plasmid

   #105729

   Purpose

   Mammalian expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

   Insert

   alpha-synuclein A30P (SNCA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A30P

   Promoter

   CMV

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH_CMV_dC_mKate2

   Plasmid

   #167336

   Purpose

   Expresses mKatet2 fused to TFAM dC-tail mutant in mammalian cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   TFAM (TFAM Human)
   

   Use

   Lentiviral

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   Truncation mutant containing MTS (1-42) as well a…

   Promoter

   CMV

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST53-LRRK2-Y1699C

   Plasmid

   #25048

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Tyrosine 1699 mutated to Cysteine

   Promoter

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pX62

   Plasmid

   #46854

   Purpose

   TOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCE

   Depositor

   Monica Hollstein

   Article

   Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

   Insert

   attB-Puro-2A and Hupki p53 G245S (TP53 Mouse, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G245S

   Promoter

   Available Since

   Aug. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX68

   Plasmid

   #46857

   Purpose

   OP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or MEFs by IMCE

   Depositor

   Monica Hollstein

   Article

   Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

   Insert

   attB-Puro-2A and Hupki p53 A138V (TP53 Mouse, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   p53 A138V

   Promoter

   Available Since

   Aug. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. ORAI1-YFP L273D-L276D

   Plasmid

   #114182

   Purpose

   ORAI1 channel with C-terminal YFP, carrying the double mutation L273D-L276D in the STIM1 binding domain, leading to a channel gating deficiency.

   Depositor

   Nicolas Demaurex

   Article

   Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

   Insert

   ORAI1-YFP L273D-L276D (ORAI1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   L273D/L276D

   Promoter

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. ORAI1-YFP V107M

   Plasmid

   #114180

   Purpose

   ORAI1 channel with C-terminal YFP, carrying the mutation V107M in the protein 1st transmembrane domain, responsible for a constitutive activity and associated with tubular aggregate myopathy (TAM).

   Depositor

   Nicolas Demaurex

   Article

   Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

   Insert

   ORAI1-YFP V107M (ORAI1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   V107M

   Promoter

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-myc-his-BACH1 K52R

    Plasmid

    #17643

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    The lysine residue at position 52 was changed to …

    Promoter

    Available Since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

11. HA-EglN3 H196A-pcDNA3

    Plasmid

    #22717

    Depositor

    William Kaelin

    Article

    Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

    Insert

    Egg laying Nine - 3 (EGLN3 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    mutant cDNA: point mutation at codon 196 was ins…

    Promoter

    Available Since

    Dec. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAcBac1-BacMam-4x-PyOtR

    Plasmid

    #217364

    Purpose

    Expresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmid

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

    Insert

    4 copies of pyrrolysyl-tRNA mutant PyOtR
    

    Use

    Baculoviral

    Tags

    Expression

    Insect and Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-EF1-FHC-POLQ-K121M

    Plasmid

    #64877

    Purpose

    lentiviral expression of human POLQ K121M mutant (a mutation in the conserved ATP-binding site of the Walker A motif in the helicase-like domain)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    K121M, a mutation in the conserved ATP-binding si…

    Promoter

    EF1alpha

    Available Since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pH6-TEV-spTorA-GFP(C)

    Plasmid

    #169041

    Purpose

    GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    H6-spTorA-GFP
    

    Use

    Tags

    6xHis, TEV cleavage site, and spTorA (signal pept…

    Expression

    Bacterial

    Mutation

    The mut3 GFP variant with 5 additional mutations …

    Promoter

    araBAD

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-FLEX-SaCas9-U6-sgGria2

    Plasmid

    #124854

    Purpose

    Mutagenesis of Gria2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria2 (Gria2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pαH-S-RRAR/D614G

    Plasmid

    #164570

    Purpose

    SARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-RRAR-D614G variant)
    

    Use

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-TEV-U1AF37MF77M

    Plasmid

    #168267

    Purpose

    Expresses human dmU1A with the F37M/F77M double mutant

    Depositor

    Joseph Wedekind

    Article

    Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

    Insert

    U1A RRM1 crystallization module (SNRPA Human)
    

    Use

    Tags

    6xHis tag N-terminus, TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    changed F37M and F77M

    Promoter

    T7 promotor

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac1_GI.1_N116C-G193C

    Plasmid

    #162580

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Asparagine 116 was mutated to Cysteine and Glycin…

    Promoter

    polyhedrin

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-mPCSK3 H194L

    Plasmid

    #122689

    Purpose

    Expresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Histidine 194 to Leucine

    Promoter

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pATZ-BFP-HA-GFP11

    Plasmid

    #177721

    Purpose

    ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFP

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Use

    Tags

    GFP11, HA, and TagBFP

    Expression

    Bacterial and Mammalian

    Mutation

    E342K (ATZ mutant)

    Promoter

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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