We narrowed to 11,993 results for: pcdna

1. pcDNA3.1-dCas9-CibN

   Plasmid

   #60552

   Purpose

   Expresses dCas9-CibN in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   dCas9-CibN
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available Since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT-TO-FH-Nsp13

   Plasmid

   #157713

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp13 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA5/FRT/TO_LAMP1_STf

   Plasmid

   #182006

   Purpose

   CMV overexpression of SNAP-tag fast in the lysosome of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   LAMP1_STf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-sACE2v2-sfGFP

   Plasmid

   #145173

   Purpose

   Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Linker GSGGSGSGG and superfolder GFP

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-sapphireAKAR-NLS

   Plasmid

   #118467

   Purpose

   cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireAKAR-NLS
   

   Use

   Tags

   Nuclear localization signal (NLS)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO-FNTB-HA

   Plasmid

   #86869

   Purpose

   To express FNTB with a HA tag at C terminus.

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   FNTB (FNTB Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-MAVS(KaganF57)

   Plasmid

   #52000

   Purpose

   Expresses the human MAVS CDS containing "weak translational context"

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   MAVS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-MS2-HA-HsMex3C_AD

   Plasmid

   #148481

   Purpose

   Mammalian Expression of HsMex3C

   Depositor

   Elisa Izaurralde

   Insert

   HsMex3C (MEX3C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L11P in Mex3C

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 FLIPglu-3.2mDelta13V

   Plasmid

   #18011

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   FLIPglu F16A S112A Delta13
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   F16A, S112A in binding protein

   Promoter

   Available Since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 FLIPE-100u

    Plasmid

    #13543

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E S118W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    S118W in binding protein

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 (-) hYAP 1-1delta

    Plasmid

    #124142

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA5/mCherry-pBirA-HA

    Plasmid

    #154458

    Purpose

    expresses mCherry-pBirA-HA/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    mCherry-pBirA-HA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F, T7 promoter

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA4/TO/Strep-HA-ZAKalpha_deltaSdeltaCTD

    Plasmid

    #141198

    Purpose

    Expresses Strep-HA-ZAKalpha_deltaSdeltaCTD in mammalian cells, can be used to make inducible cell lines

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Use

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    deletion of last 27 amino acids and amino acids 6…

    Promoter

    CMV

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 ALK4 K234R

    Plasmid

    #80881

    Purpose

    mammalian expression of ALK4 K234R

    Depositor

    Peter ten Dijke, Aristidis Moustakas

    Article

    ten Dijke et al Science. 1994 Apr 1;264(5155):101-4.

    Insert

    ALK4 (ACVR1B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K234R

    Promoter

    CMV

    Available Since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3 Flag Jnk1a1MKK7B2

    Plasmid

    #19731

    Depositor

    Roger Davis

    Article

    Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

    Insert

    Jnk1a1MKK7b2 (Map2k7, MAPK8 Human, Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1/HisC 14-3-3 epsilon wt

    Plasmid

    #11942

    Depositor

    Michael Yaffe

    Article

    Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

    Insert

    Ywhae (Ywhae Rat)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA-Flag-LmoCas6

    Plasmid

    #126485

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas6
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 Flag PA1

    Plasmid

    #15559

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    PA1 (PAGR1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pCDNA4.TO-ORF68-2xCSTREP

    Plasmid

    #136229

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF68

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF68
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-TOP luciferase

    Plasmid

    #26611

    Depositor

    Jie Chen

    Article

    Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

    Insert

    eEF2 5'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only