We narrowed to 14,223 results for: Cas9
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Plasmid#140206PurposeLenti Crispr/Cas9 with mOrange fluorescent markerDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterpELF1aAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pMCSG7-dNmeCas9
Plasmid#71475Purposebacterial pMCSG7 expression vector expressing catalytically inactive Nme cas9 with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-Inactive-NmeCas9
Tags6HisExpressionBacterialMutationD16A H588AAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX458-DsRed2
Plasmid#112219PurposeCloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2DepositorInsertDsRed2
UseCRISPRExpressionMammalianMutationDsRed2 contains a silent nucleotide substitution …Available sinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCSD_SpCas9MT3-NLS-3xHA-NLS-SaCas9WT-2xNLS
Plasmid#107322PurposeExpresses SpCas9 (R1335K) fused to SaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1335K) is fused to SaCas9PromoterCMV IE93Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX459-CDKN1A
Plasmid#217457PurposeFor CDKN1A (p21) knockoutDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRISPR Cas9 guide for CDKN1A (CDKN1A Human)
UseCRISPRExpressionMammalianAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En03
Plasmid#71535PurposeUsed to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hRad51(A190L-A192L)–Cas9(D10A)
Plasmid#125562PurposeExpresses the Rad51(A190L-A192L)–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRad51(A190L A192L)–Cas9(D10A) (RAD51 Human)
UseCRISPRExpressionMammalianAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas9m3
Plasmid#47315PurposeCas9 D10A+D839A+H840ADepositorInsertCas9m3
UseCRISPRAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2S293A
Plasmid#115204PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S293A (PDHA2 Human)
UseLentiviralMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2
Plasmid#115199PurposeLentiviral transduction and expression of a CRISPR/Cas9-resistant PDHA2 into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2WT (PDHA2 Human)
UseLentiviralMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2S291A
Plasmid#115203PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S291A (PDHA2 Human)
UseLentiviralMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA
Plasmid#110624PurposeA single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNADepositorInsertsSt1Cas9 LMD-9
sgRNA for St1Cas9
UseAAV, CRISPR, and Mouse TargetingTagsSV40 NLSExpressionMammalianPromoterLP1B and hU6Available sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
hRad51(F86E)–Cas9(D10A)
Plasmid#125564PurposeExpresses the Rad51(F86E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
hRad51(G151D)–Cas9(D10A)
Plasmid#125565PurposeExpresses the Rad51(G151D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRad51(G151D)–Cas9(D10A) (RAD51 Human)
UseCRISPRExpressionMammalianAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLH02
Plasmid#117262PurposeGenome editing for Lactobacillus brevis ATCC367DepositorInsertPorfX-LVISKB_1732-30 , sapK-sappR
UseOtherAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDual_dsCas9_BFP
Plasmid#214679PurposeLentiviral expression vector for an inducible Cas9-P2A-BFP with two empty sgRNA cloning sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCP-tRNA
Plasmid#133812PurposeCRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosisDepositorInsertcassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
UseCRISPRAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDonor-tBFP-NLS-Neo
Plasmid#80766PurposeCustomizable donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.DepositorTypeEmpty backboneUseCRISPRTagsTagBFP2-3×NLSExpressionMammalianAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pIgnaviCas9
Plasmid#127595PurposeExpresses IgnaviCas9 in E. coliDepositorInsertIgnaviCas9
Tags6xHis Tag and Maltose binding proteinExpressionBacterialPromoterT7 promoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZA31-sulA-GFP
Plasmid#78493PurposeExpression of GFP under the sulA promoter.DepositorInsertGFPmut2
ExpressionBacterialAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by