We narrowed to 3,022 results for: ER

1. Ii-Str_ST-SBP-mCherry

   Plasmid

   #65269

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ii-Streptavidin and truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBiex PKC - Peptide 8 GFP RFP

   Plasmid

   #83564

   Purpose

   Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

   Insert

   PKC alpha (PRKCA Human)
   

   Use

   Tags

   10 nm ER/K Helix, FLAG purification tag, TEV Prot…

   Expression

   Bacterial and Insect

   Mutation

   Only catalytic domain of PKC (aa 335-672)

   Promoter

   T7 Promoter

   Available since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-C1-FKBP-CYB5tail

   Plasmid

   #202686

   Purpose

   rapamycin-induced recruitment of proteins to ER

   Depositor

   Gerry Hammond

   Article

   Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

   Insert

   EGFP:FKBP:[GGSA]4GG:CYB5A(100-134) (CYB5A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)

   Plasmid

   #47542

   Purpose

   engineered receptor improves heteromeric receptor expression and sensitivity to IVM.

   Depositor

   Henry Lester

   Article

   Frazier et al J Biol Chem. 2013 May 29.

   Insert

   Glutamate-gated chloride channels
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   retention motif in ER (starting at R340) changed …

   Promoter

   CMV

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. Str-KDEL_ST-SBP-tagBFP

   Plasmid

   #65266

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and tagBFP

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-H2B-mRuby3-IRES-eGtACR1-ST

   Plasmid

   #108960

   Purpose

   Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   eGtACR1-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag- mCry1ER-pBABEpuro

   Plasmid

   #61429

   Purpose

   Express mCRY1-ER* fusion protein

   Depositor

   Aziz Sancar

   Article

   Ye et al Genes Dev. 2014 Sep 15;28(18):1989-98. d

   Insert

   mCry1 (Cry1 Mouse)
   

   Use

   Retroviral

   Tags

   Flag and mouse estrogen receptor ligand binding d…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pBiex PKC - Peptide 8 mCit mCer

   Plasmid

   #83563

   Purpose

   Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

   Insert

   PRKCA (PRKCA Human)
   

   Use

   Tags

   10 nm ER/K Helix, FLAG purification tag, TEV Prot…

   Expression

   Bacterial and Insect

   Mutation

   Only catalytic domain of PKC (aa 335-672)

   Promoter

   T7 Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. MCP-mCherry-SEC61B

   Plasmid

   #207671

   Purpose

   To express fluorescently labeled MS2 coat binding protein (MCP) fused with ER resident protein SEC61B

   Depositor

   Christine Mayr

   Article

   Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

   Insert

   SEC61B (SEC61B Human)
   

   Use

   Tags

   MCP-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-CAG-DIO-erHRP(N175S mutant)

    Plasmid

    #79906

    Purpose

    Adeno-associated virus plasmid, Cre-dependent FLEX switch, erHRP(N175S mutant)

    Depositor

    Joshua Sanes

    Article

    Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

    Insert

    erHRP(N175S mutant)
    

    Use

    AAV and Cre/Lox

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    N175S mutation

    Promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. EC71167

    Plasmid

    #154066

    Purpose

    Level 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFP

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    ZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    All BsaI, Esp3I and BPiI restriction sites were r…

    Promoter

    Zea mays Ubiquitin Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS405 SEC66-VAC8-RFP

    Plasmid

    #166843

    Purpose

    Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8, SEC66 Budding Yeast)
    

    Use

    Tags

    RFP and SEC66 transmembrane domain

    Expression

    Yeast

    Mutation

    SEC66 transmembrane domain

    Promoter

    VAC8 promoter

    Available since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-X1-Neo-GFP-ATL2-deltaTM

    Plasmid

    #109021

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL2-deltaTM (ATL2 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    truncated form without C-terminal ER transmembran…

    Promoter

    EIF-1a promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX 4T1 ERbeta(144-530)

    Plasmid

    #35566

    Depositor

    Harish Srinivas

    Article

    Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

    Insert

    Estrogen Receptor beta (ESR2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    residues 144-530

    Promoter

    Available since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pMSCV-Atoh1ER-IRES-GFP

    Plasmid

    #33335

    Depositor

    Charles Sherr, Martine Roussel

    Article

    Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Use

    Retroviral

    Tags

    ER

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pGEX 4T1 ERbeta(144-255)

    Plasmid

    #35565

    Depositor

    Harish Srinivas

    Article

    Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

    Insert

    Estrogen Receptor beta (ESR2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    residues 144-255

    Promoter

    Available since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pGEX 4T1 ERbeta(1-255)

    Plasmid

    #35564

    Depositor

    Harish Srinivas

    Article

    Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

    Insert

    Estrogen Receptor beta (ESR2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    residues 1-255

    Promoter

    Available since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pET28a-MBP-(CBX1)3-eReader

    Plasmid

    #135090

    Purpose

    Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1, engineered x3
    

    Use

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    lysine 43 mutated to alanine; aspartic acid 59 mu…

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTK3a_006

    Plasmid

    #123759

    Purpose

    Encodes ER Targeting Sequence (N Terminal) as a Type 3a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    ETS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 373_ESR1 in pmiRGlo

    Plasmid

    #78134

    Purpose

    luciferase reporter for miRNA activity

    Depositor

    Heidi Schwarzenbach

    Article

    Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    3'UTR containing miRNA binding sites

    Promoter

    PGK

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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