We narrowed to 14,031 results for: TIM

1. Plasmid L5 attB:Psmyc triple red

   Plasmid

   #169413

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for triple red

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   mScarlet proper
   COmScarlet
   mRuby3
   

   Use

   Unspecified

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pluc2-iRFP720

   Plasmid

   #98887

   Purpose

   Mammalian expression

   Depositor

   Laura Mezzanotte

   Article

   Mezzanotte et al Cell Transplant. 2017 Apr 26. doi: 10.37

   Insert

   Firefly Luciferase
   

   Tags

   iRFP720

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. LT2_12_GlyRNA

   Plasmid

   #177710

   Purpose

   Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Inserts

   pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
   pTEFmut8-mCherry-tADH1
   

   Use

   Used as donor dna in genome integration after lin…

   Expression

   Yeast

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1-CAMKIIbeta(T287D)

   Plasmid

   #21223

   Depositor

   Tobias Meyer

   Article

   Shen et al Science. 1999 Apr 2. 284(5411):162-6.

   Insert

   CAMKIIbeta (Camk2b Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CAMKIIbeta(T287D) Lab plasmid CF 0001.

   Available Since

   July 29, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pTol2-nbt-Electra2-P2A-mScarlet

   Plasmid

   #196114

   Purpose

   zebrafish expression of nbt-Electra2-P2A-mScarlet

   Depositor

   Reinhard Köster

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   nbt-Electra2-P2A-mScarlet
   

   Use

   Zebrafish expression

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pH2B-Electra1

   Plasmid

   #179478

   Purpose

   Nuclear labeling with blue fluorescent protein Electra1 fused to H2B

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   H2B-Electra1
   

   Tags

   Electra1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCK01_PalkS_alkS

   Plasmid

   #166502

   Purpose

   Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.

   Depositor

   Sven Panke

   Article

   Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

   Insert

   alkS
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   L401G

   Promoter

   PalkS

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJIT163-2NLSCas9

   Plasmid

   #53064

   Purpose

   Expression of rice codon-optimized Cas9 in plant cells

   Depositor

   Caixia Gao

   Article

   Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

   Insert

   Cas9
   

   Use

   Plant expression

   Mutation

   Rice codon optimized

   Promoter

   2x35S

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-V5-hTEV

   Plasmid

   #65800

   Purpose

   expresses human codon-optimised TEV protease

   Depositor

   Douglas Green

   Article

   Oberst et al J Biol Chem. 2010 May 28;285(22):16632-4

   Insert

   V5-human TEV
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   codon-optimised to express in mammalian cells

   Promoter

   CMV

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCytERM-Electra2

    Plasmid

    #179477

    Purpose

    ER Visualization (OSER assay) Expresses Electra2 in fusion with N-terminal part of p450

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    CytERM-Electra2
    

    Tags

    Electra2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb

    Plasmid

    #182009

    Purpose

    CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    IgKchL_HA_STf_myc_PDGFRtmb
    

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-ChR2-mRuby2-ST

    Plasmid

    #109125

    Purpose

    Cation channelrhodopsin ChR2 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChR2-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKeratin-Electra2

    Plasmid

    #184928

    Purpose

    Keratin labeling with blue fluorescent protein Electra2

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Keratin-Electra2
    

    Tags

    Electra2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1457_pDEST_miniTol2_R4-R2_Cryst_mCherry

    Plasmid

    #171796

    Purpose

    pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC1749

    Plasmid

    #127983

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-HkCas12a-2AeGFP

    Plasmid

    #123634

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    HkCas12a
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJ607-CMV-GFP-2A-Cas9

    Plasmid

    #154830

    Purpose

    GFP-2A-Cas9 (CHO codon-optimized)

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Inserts

    GFP-2A-Cas9
    HygR
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Codon-optimized (Cricetulus griseus)

    Promoter

    CMV and SV40 promoter

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. mNG-sfTq2ox

    Plasmid

    #124219

    Purpose

    Cytoplasmic mNeongreen superfolder mTurquoise2 ox tandem, ~60 % energy transfer. pNM045 - pSAV057-mNG-sfTq2ox

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    mNG-sfTq2ox
    

    Expression

    Mammalian

    Available Since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. TagRFP-T-VAPB(1- 218)- eMagB

    Plasmid

    #162255

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    TagRFP-T-VAPB(1- 218)- eMagB
    

    Expression

    Mammalian

    Promoter

    beta-actin promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT3TS_Transposase

    Plasmid

    #109768

    Purpose

    Codon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Tol2 Transposase
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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