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pGL5

Plasmid

#48745

Purpose

Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +2064 with respect to transcription start site at +1)

Depositor

Joseph Takahashi

Article

Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

Insert

truncated mPer2 promoter/enhancer region (-1128 to +2064)

Use

Luciferase

Mutation

truncated promoter region (see pGL6)

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-GST-mEKLF

Plasmid

#67836

Purpose

Encodes GST fusion to murine EKLF (nucleotides 71-1215)

Depositor

James Bieker

Article

Zhang et al Proc Natl Acad Sci U S A. 1998 Aug 18;95

Insert

EKLF (Klf1 Mouse)

Expression

Bacterial

Mutation

Full-length mEKLF is aa 20-376; amino acid 19 is …

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pRVdG-4FLPo

Plasmid

#122050

Purpose

Genome plasmid for deletion-mutant rabies virus encoding Flpo recombinase

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

FLPo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Nuc-Orange Nano-lantern(Ca2+)/pcDNA3

Plasmid

#71349

Purpose

Express Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern(Ca2+)_H2B

Expression

Mammalian

Available Since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18

Plasmid

#172317

Purpose

CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs

Depositor

Steven Jacobsen

Article

Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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815 pSG5L HA PTEN delta274-342

Plasmid

#10738

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of amino acids 274-342.

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
AAEL007584-Cas9

Plasmid

#100706

Purpose

The promoter of AAEL007584 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

Inserts

AAEL007584-hspcas9-GFP
Opie2-dsRed

Use

CRISPR

Tags

GFP and dsRED

Expression

Insect

Promoter

AAEL007584 and Opie2

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCNL-C1_RhoB

Plasmid

#65697

Purpose

Expresses CNL-RhoB in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

RhoB (RHOB Human)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only
pCR4_SNORD27 probe

Plasmid

#73069

Purpose

antisense probe for detection of human SNORD27

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (SNORD2 Human)

Use

In vitro transcription

Promoter

t7

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2 Krt4 Src (YF)-uniRapR-cerulean

Plasmid

#45509

Depositor

Klaus Hahn, Anna Huttenlocher

Article

Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

Src (YF)-uniRapR-cerulean

Use

Zebrafish expression

Tags

cerulean

Mutation

Y732F

Promoter

krt4

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pET15b-GB

Plasmid

#129689

Purpose

express E. coli GreB

Depositor

Jeff Gelles

Article

Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

his6/thrombin/GreB (greB E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-XPLN aa452-526

Plasmid

#73381

Purpose

Expresses amino acids 452-526 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 452-526 of XPLN (arhgef3) p…

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa304-466 (PH)

Plasmid

#73380

Purpose

Expresses amino acids 304-466 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 304-466 of XPLN (arhgef3) p…

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa107-311

Plasmid

#73379

Purpose

Expresses amino acids 107-317 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 107-317 of XPLN (arhgef3) p…

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa1-125 (N125)

Plasmid

#73378

Purpose

Expression of the N-terminal region of XPLN (amino acids 1–125, designated N125)

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses N terminal amino acid 1-125 of XPLN (ar…

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
824 pGEX2T PTEN delta274-342

Plasmid

#10739

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Bacterial

Mutation

Deletion of amino acids 274-342.

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
QuasAr-P2A-GCaMP

Plasmid

#154082

Purpose

Independent expression of QuasAr and GCaMP under human synapsin 1 promoter

Depositor

Michael Hoppa

Article

Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

Insert

QuasAr-P2A-GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

Changed Tyrosine 327 to Alanine

Promoter

human synapsin 1

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBT310_pBS-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52543

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo
PhiC31o integrase

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCDEF3‐mTim1 (no tag)

Plasmid

#49206

Purpose

expresses mouse Tim1

Depositor

Lawrence Kane

Article

de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

Insert

Tim1 (Havcr1 Mouse)

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only
P6598 MSCV-IP N-HAonly TBPL1

Plasmid

#34896

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

TBPL1 (TBPL1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only