We narrowed to 73,945 results for: LAS

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pBPK1520-SNCA-A53T-ng

Plasmid

#181739

Purpose

Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A53T mutation in PE3 approach

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing ngRNA for SNCA-A53T (SNCA Human)

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_FNDC3B

Plasmid

#45269

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

FNDC3B (FNDC3B Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pENTR-E2F1

Plasmid

#37141

Depositor

Robert Duronio

Article

Shibutani et al Dev Cell. 2008 Dec;15(6):890-900.

Insert

E2F1 (E2f1 Fly)

Use

Gateway

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-UCHL5

Plasmid

#31219

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

UCHL5 (UCHL5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Aug. 19, 2011

Availability

Academic Institutions and Nonprofits only
pLenti6/V5-DEST-NDC80

Plasmid

#31215

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

NDC80 (NDC80 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
ABE8.8-d

Plasmid

#136295

Purpose

expresses ABE8.8-d in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.8-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-SNCA-A30P

Plasmid

#180016

Purpose

Transiently expressing a pegRNA to introduce SNCA-A30P mutation in human cells

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for SNCA-A30P (SNCA Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBPK1520-SNCA-A30P-ng

Plasmid

#180018

Purpose

Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A30P mutation in PE3 approach

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing ngRNA for SNCA-A30P (SNCA Human)

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_Flag_TP53_P278A

Plasmid

#183113

Purpose

Expresses flag-tagged p53 with P278A mutation in mammalian cells

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed proline 278 to alanine, see depositor com…

Promoter

EF1a

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_EIF5A2

Plasmid

#45266

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

EIF5A2 (EIF5A2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_ZKSCAN1_Tau7-12WT

Plasmid

#194166

Purpose

Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 7,9-12. Expresses the tau circRNA 12-->7 WT with 3X flag tag in exon 7.

Depositor

Stefan Stamm

Article

Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

Insert

Microtubule-associated protein tau 7-12 WT

Tags

3X Flag

Expression

Mammalian

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ZKSCAN1_Tau10-12WT

Plasmid

#194163

Purpose

Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 10-12. Expresses the tau circRNA 12-->10 WT with 3X flag tag in exon 10.

Depositor

Stefan Stamm

Article

Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

Insert

Microtubule-associated protein tau 10-12 WT ZKSCAN1 intronic alu elements

Tags

3X flag tag

Expression

Mammalian

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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PARD6A_PDZ_1

Plasmid

#103946

Purpose

Protein expression and purification of TAX INTERACTION PROTEIN PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PARD6A (PARD6A Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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Mouse DsRed-Monomer CASP9 C325A Mutant

Plasmid

#198401

Purpose

Expresses Mouse DsRed-Monomer CASP9 C325A Mutant Fusion Protein in Mammalian Cells

Depositor

Hannah Rabinowich

Article

Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

Insert

Mouse Caspase-9 C325A Mutant (Casp9 Mouse)

Tags

DsRed-monomer

Expression

Mammalian

Mutation

Changed Cysteine 325 to Alanine to eliminate prot…

Promoter

CMV

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9-Puro HLAG-2B-sgRNA

Plasmid

#182552

Purpose

Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

Depositor

Santiago Miriuka

Article

Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

Insert

hSpCas9-2A-Puro-HLAG-2B-sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

Cbh

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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LIN7A_PDZ_1

Plasmid

#103918

Purpose

Protein expression and purification of LIN7 PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

LIN7A (LIN7A Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shStk16-1

Plasmid

#180391

Purpose

Producing AAV that encodes mouse Stk16 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shStk16-1 (Stk16 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_GPR160

Plasmid

#45274

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

GPR160 (GPR160 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pHR_UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry

Plasmid

#188770

Purpose

dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

dCas9-XTEN80-KRAB(Kox1)

Use

Lentiviral

Tags

HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Promoter

SFFV

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pWzl_neo_DEST_flag_SOX2

Plasmid

#45309

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

MCOPS3 (SOX2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pEN_hU6miR-G12-E

Plasmid

#25757

Purpose

Entry vector with human U6 promoter driving mouse G alpha 12 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Entry vector

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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DLG3_PDZ_3

Plasmid

#103948

Purpose

Protein expression and purification of NE-DLG PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

DLG3 (DLG3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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STT3A pmRFP (663)

Plasmid

#61997

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

STT3A (STT3A Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
mCherry-FKBP-TPD54(L53P,L67P)

Plasmid

#172458

Purpose

Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag L53P,L67P mutant

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD54 (TPD52L2 Human)

Tags

mCherry-FKBP

Expression

Mammalian

Mutation

changed Leucines 53 and 67 to proline (breaks coi…

Promoter

CMV

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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PTPN13_PDZ_3

Plasmid

#103900

Purpose

Protein expression and purification of PTPN13 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MAGI1 (MAGI1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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MAGI1_PDZ_2

Plasmid

#103899

Purpose

Protein expression and purification of MAGI1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MAGI1 (MAGI1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK-Neo-TGmiR-Gb2

Plasmid

#25746

Purpose

Control lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 (Gnb2 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pWzl_neo_DEST_flag_PIK3CA

Plasmid

#45308

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
ABE8.13-d

Plasmid

#136297

Purpose

expresses ABE8.13-d in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.13-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
USH1C_PDZ_1

Plasmid

#103936

Purpose

Protein expression and purification of P73 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

USH1C (USH1C Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge