We narrowed to 7,518 results for: Lif

1. pcDNA5/FRT/TO_Lyn11-HaloTag7

   Plasmid

   #175533

   Purpose

   Expression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lyn11-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5/FRT/TO_Lyn11-HaloTag9

   Plasmid

   #175543

   Purpose

   Expression of HaloTag9 at the inner leaflet of the plasma membrane of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lyn11-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSKI074

   Plasmid

   #11571

   Depositor

   Detlef Weigel

   Article

   Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

   Insert

   CaMV 35S enhancers
   

   Use

   Activation tagging

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Flag-MALT1-/pRK5

   Plasmid

   #27971

   Depositor

   Xiaolu Yang

   Article

   Kawadler et al Mol Cell. 2008 Aug 8. 31(3):415-21.

   Insert

   MALT1 (MALT1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pLX-hPGK_NKX2-1

   Plasmid

   #221494

   Purpose

   NKX2-1 short isoform lentiviral overexpression using hPGK promoter

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV5 HA FoxO1

   Plasmid

   #14936

   Depositor

   Joan Massague

   Article

   Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

   Insert

   FoxO1 (Foxo1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA5/FRT/TO_HaloTag9_SKL

   Plasmid

   #175542

   Purpose

   Peroxysomal expression of HaloTag9 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   HaloTag9-SKL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLH-sgRNA-Sirius-8XMS2

   Plasmid

   #121939

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   sgRNA-Sirius-8XMS2
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPLP1H-mCf

   Plasmid

   #229984

   Purpose

   LIC vector for polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.

   Depositor

   Barry Lutz

   Article

   Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPLP2H-mCf

    Plasmid

    #229985

    Purpose

    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.

    Depositor

    Barry Lutz

    Article

    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPLP3H-mCf

    Plasmid

    #229987

    Purpose

    LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.

    Depositor

    Barry Lutz

    Article

    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPUR-mU6-sgRNA-Sirius-8XPP7

    Plasmid

    #121943

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XPP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLX-EF1a_NKX2-1

    Plasmid

    #221484

    Purpose

    NKX2-1 short isoform lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro

    Plasmid

    #60909

    Purpose

    A minimal K560 kinesin rigor motor tagged with the SunTag for single molecule imaging

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    K560-E236A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHAGE-TO-dCas9-P2A-HSA

    Plasmid

    #121936

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    dCas9-P2A-HSA
    

    Use

    CRISPR and Lentiviral

    Tags

    HSA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ttAtCas12a+int

    Plasmid

    #182384

    Purpose

    Gives very high editing efficiency in barley. Also works in Brassica oleracea

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al bioRxiv 2022.04.28.489853

    Insert

    LbCas12a coding sequence with D156R and Arabidopsis introns
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D156R + 8 Arabidopsis introns added

    Promoter

    None

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

    Plasmid

    #175529

    Purpose

    Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Cox8a-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p159 pPr-luc

    Plasmid

    #8392

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    probasin promoter (Pbsn Rat)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 29, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pCMU-ACTLr

    Plasmid

    #61193

    Purpose

    Fluorescent reporter for F-actin LifeAct-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    LifeAct-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Flag FoxO3

    Plasmid

    #14937

    Depositor

    Joan Massague

    Article

    Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

    Insert

    FoxO3 (FOXO3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-AncMEcoGII291

    Plasmid

    #205024

    Purpose

    Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 291
    

    Use

    Tags

    His6x tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA5/FRT/TO_NES_HaloTag9

    Plasmid

    #175537

    Purpose

    Strict cytosolic expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    NES-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBAD/HisB (TIREVOL)-sfGFP

    Plasmid

    #189675

    Purpose

    titratable expression of sfGFP in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    sfGFP
    

    Use

    Tags

    His-T7-EK

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLV-hCDC6p-Venus

    Plasmid

    #212665

    Purpose

    Express Venus under CDC6 promoter

    Depositor

    Yumi Konagaya, Tobias Meyer

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    hCDC6p-Venus
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hCDC6p

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

    Plasmid

    #175534

    Purpose

    Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    IgKchL_HA-HaloTag7-myc_PDGFRtmb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGFP-E p27 (CC#631)

    Plasmid

    #15192

    Depositor

    Connie Cepko

    Article

    Dyer et al J Neurosci. 2001 Jun 15. 21(12):4259-71.

    Insert

    p27 (Cdkn1b Mouse)
    

    Use

    Retroviral

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLH-sgRNA-Sirius-8XPP7

    Plasmid

    #121940

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XPP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a-AncMEcoGII250

    Plasmid

    #205025

    Purpose

    Expressing engineered M.EcoGII with higher thermostability

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 250
    

    Use

    Tags

    His6x tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-CyRFP1

    Plasmid

    #84356

    Purpose

    Expresses CyRFP1 fluorescent protein in an empty backbone, under CAG promoter

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    CyRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3 CDK8 HA (P#634)

    Plasmid

    #14649

    Depositor

    Matija Peterlin

    Article

    Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

    Insert

    Cdk8 (CDK8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge