We narrowed to 4,867 results for: gne

1. pAAV-CMV-rtTA-IRES-GFP-WPRE-pA

   Plasmid

   #183709

   Purpose

   AAV designed to express rtTA and eGFP from a CMV promoter

   Depositor

   Jonathan Ploski

   Insert

   rtTA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pANY8

   Plasmid

   #164900

   Purpose

   Containing His-ELP-intein multifunctional tag for protein purification by combining Ni-NTA-based and ELP-based strategy with a specially designed purification protocol.

   Depositor

   Yingfeng An

   Insert

   ccdB cassette
   

   Use

   Tags

   His-ELP-Intein-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. UC20m

   Plasmid

   #121040

   Purpose

   MoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).

   Depositor

   Richard Murray

   Insert

   gN2 Cas9 gRNA UC part
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiUBC CASP1[CARD]-mSG2 (inflammasome reporter)

   Plasmid

   #228044

   Purpose

   Lentiviral stable Inflammasome speck reporter, designed from PMC9623368.

   Depositor

   Wade Harper

   Insert

   CASP1[CARD]-mSG2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223 CASP1[CARD]-mSG2 (inflammasome reporter)

   Plasmid

   #228043

   Purpose

   DONR vector for inflammasome speck reporter, designed from PMC9623368.

   Depositor

   Wade Harper

   Insert

   CASP1[CARD]-mSG2
   

   Use

   Gateway donr

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. EP001: pLenti CMV Blast::eGFP-RC-I-1-H11

   Plasmid

   #209301

   Purpose

   Expression plasmid for RC-I-1-H11 (de novo virus-like particle designed by David Baker lab) with N-terminal eGFP

   Depositor

   Adam Cohen

   Insert

   RC-I-1-H11
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. EP002: pLenti CMV Blast::HaloTag-RC-I-1-H11

   Plasmid

   #209302

   Purpose

   Expression plasmid for RC-I-1-H11 (de novo virus-like particle designed by David Baker lab) with N-terminal HaloTag

   Depositor

   Adam Cohen

   Insert

   RC-I-1-H11
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-L1PA1CH_blast rescue

   Plasmid

   #69611

   Purpose

   Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts

   Depositor

   Astrid Roy-Engel

   Insert

   L1PA1
   

   Use

   Tags

   blast rescue cassette

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUS252y

   Plasmid

   #191834

   Purpose

   Expresses fuYFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuYFP to place in other plasmid backbones.

   Depositor

   Nicholas Coleman

   Insert

   fuYFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pUS252L

    Plasmid

    #191832

    Purpose

    Expresses fuBFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuBFP to place in other plasmid backbones

    Depositor

    Nicholas Coleman

    Insert

    fuBFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pUS252b

    Plasmid

    #191831

    Purpose

    Expresses fuGFPb constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPb to place in other plasmid backbones

    Depositor

    Nicholas Coleman

    Insert

    fuGFPb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pUS252s

    Plasmid

    #191833

    Purpose

    Expresses fuGFPs constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPs to place in other plasmid backbones

    Depositor

    Nicholas Coleman

    Insert

    fuGFPs
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pAIP1

    Plasmid

    #233685

    Purpose

    Engineered SUMO protease expression plasmid designed for efficient protease-mediated elution.

    Depositor

    Christopher Bahl

    Insert

    Ulp1-like SUMO protease
    

    Use

    Tags

    CAHS-107838 and HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pUS252

    Plasmid

    #127674

    Purpose

    Expresses fuGFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFP to place in other plasmid backbones

    Depositor

    Nicholas Coleman

    Insert

    Free Use GFP (fuGFP)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    June 24, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pMulti_K_ccdB

    Plasmid

    #223852

    Purpose

    GEC-acceptor for 4G-cloning, designed for expression in E. coli (Kanamyicin resistance)

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Type

    Empty backbone

    Use

    Golden-gate acceptor

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMulti_S_ccdB

    Plasmid

    #223853

    Purpose

    GEC-acceptor for 4G-cloning, designed for expression in E. coli (Streptomycin resistance)

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Type

    Empty backbone

    Use

    Golden-gate acceptor

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMulti_A_ccdB

    Plasmid

    #223851

    Purpose

    GEC-acceptor for 4G-cloning, designed for expression in E. coli (Ampicillin resistance)

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Type

    Empty backbone

    Use

    Golden-gate acceptor

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. StarPort01

    Plasmid

    #164503

    Purpose

    To generate a stable acceptor cell line with a BxbI attP site at the AASV1 locus, between an exogenous human pRPL30 promoter and a tripartite V5/SNAPtag/Blast reporter.

    Depositor

    Bo Huang

    Insert

    V5-SNAPtag-PDGFR_TM-T2A-Blast
    

    Use

    To generate attp acceptor cell lines for bxbi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human RPL30

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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19. StarPort02

    Plasmid

    #164504

    Purpose

    To generate a stable acceptor cell line with an improved BxbI attP-GA site at the AASV1 locus, between an exogenous human pRPL30 promoter and a tripartite V5/SNAPtag/Blast reporter.

    Depositor

    Bo Huang

    Insert

    V5-SNAPtag-PDGFR_TM-T2A-Blast
    

    Use

    To generate attp-ga acceptor cell lines for bxbi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human RPL30

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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20. hAAVS1 1R TALEN

    Plasmid

    #35432

    Purpose

    TALEN designed to target the human AAVS1 locus

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    hAAVS1 1R TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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