59,474 results

1. pDualIN-SMN2

   Plasmid

   #214430

   Purpose

   Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

   Insert

   SMN2 EXON 7 (SMN2 Human)
   

   Expression

   Mammalian

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. ST-GFP

   Plasmid

   #162500

   Purpose

   Expresses ST6GAL1 with a GFP tag in its C-terminus in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   ST6Gal1 (ST6GAL1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX305_3xFLAG_RELA1

   Plasmid

   #220324

   Purpose

   Expresses full length human RELA with an N-terminal 3xFLAG tag

   Depositor

   Jonathan Brown

   Insert

   RELA (RELA Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Promoter

   hPGK

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. WT FL USP7

   Plasmid

   #227214

   Purpose

   Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Tags

   6His

   Expression

   Bacterial

   Promoter

   T7-promoter

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 - hSRSF6

   Plasmid

   #120419

   Purpose

   overexpression of human SRSF6

   Depositor

   Gillian Bates, Andreas Neueder

   Article

   Neueder et al Nat Commun. 2018 Sep 27;9(1):3955. doi:

   Insert

   SRSF6 (SRSF6 Human)
   

   Expression

   Mammalian

   Promoter

   CMV immediate/early

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1+U1snRNA

   Plasmid

   #194202

   Purpose

   Expresses U1 snRNA

   Depositor

   Ralf Brandes, Matthias Leisegang

   Insert

   U1 snRNA (RNU1-1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nrf1-Myc pcDNA3.1(+)-C-Myc

   Plasmid

   #169125

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hNrf1 (NFE2L1 Human)
   

   Expression

   Mammalian

   Mutation

   wt

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. TJP1_PDZ_1

   Plasmid

   #103909

   Purpose

   Protein expression and purification of ZO1 PDZ1 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   TJP1 (TJP1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. IDG_CACNA2D2_OE_1

   Plasmid

   #161616

   Purpose

   Overexpresses CACNA2D2

   Depositor

   Mike McManus

   Insert

   CACNA2D2 (CACNA2D2 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE-60 Grx1-roGFP2-His

    Plasmid

    #64799

    Purpose

    Bacterial expression of His-tagged Grx1-roGFP2

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Tags

    His and roGFP2

    Expression

    Bacterial

    Available Since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRIPZ shcGAS-Hsa

    Plasmid

    #128175

    Purpose

    Doxycyclin inducible shRNA knockdown of human cGAS gene

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shcGAS Mmu (CGAS Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUW-tetO-NFIA

    Plasmid

    #141403

    Purpose

    Inducible Expression of NFIA

    Depositor

    Lorenz Studer

    Article

    Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

    Insert

    NFIA (NFIA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-EF1a-GFP-SFPQY527A-IRES-mCherry

    Plasmid

    #166951

    Purpose

    Lentiviral plasmid expressing GFP-tagged SFPQ Y527A protein with IRES-mCherry from the EF1a promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ-Y527A (SFPQ Human)
    

    Use

    Lentiviral

    Tags

    GFP and mCherry

    Mutation

    SFPQ-Y527A

    Promoter

    eF1a

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP.N3-SLC35A1-GFP

    Plasmid

    #186281

    Purpose

    transient expression of C-terminal GFP tagged SLC35A1 isoform a in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

    Insert

    SLC35A1 (SLC35A1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBMN-YFP-Parkin

    Plasmid

    #59416

    Purpose

    to make YFP-Parkin stable cell line with retrovirus

    Depositor

    Richard Youle

    Article

    Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

    Insert

    YFP-Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. THBS1-bio-His

    Plasmid

    #53417

    Purpose

    Expresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    THBS1 (THBS1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. px458_2A_GFP_sgRNA_LTN1

    Plasmid

    #127125

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA LTN1 (LTN1 Human)
    

    Use

    CRISPR

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-GFP-AURKA-mCherry

    Plasmid

    #99876

    Purpose

    Expression of GFP-AURKA-mCherry for protein purification in bacteria

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP and mCherry

    Expression

    Bacterial

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFUW-tetO-NFKB1

    Plasmid

    #139822

    Purpose

    doxycycline-inducible expression of Human NFKB1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    NFKB1 (NFKB1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Braf

    Plasmid

    #40775

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    Braf (BRAF Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Starting Met removed

    Promoter

    CMV

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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