We narrowed to 20,285 results for: ACE;

1. pCk1

   Plasmid

   #136695

   Purpose

   Acceptor plasmid for odd level position 1, lacZ blue-white screening. Part of the OpenPlant toolkit.

   Depositor

   Nicola Patron

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. attB-P7-attP-YFP

   Plasmid

   #195571

   Purpose

   A strong P7 promoter flanked by Bxb1 recombination sites attB and attP. Downstream of attP is a strong RBS and YFP coding sequence.

   Depositor

   Richard Murray

   Article

   Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52

   Insert

   Promoter flanked by Bxb1 recombination sites attB and attP with YFP downstream
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   P7

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAcBac1-3nitroY-A7-RS

   Plasmid

   #141173

   Purpose

   Expression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acids

   Depositor

   Ryan Mehl

   Article

   Porter et al ACS Chem Biol. 2019 Jun 21;14(6):1328-13

   Insert

   A7 3nY tRNA synthetase
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   P186T L274R N311G C313N

   Promoter

   CMV

   Available Since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNOC-CRISPR-35S

   Plasmid

   #99861

   Purpose

   Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   Cas9-Nlux

   Expression

   Mutation

   Promoter

   Ribi promoter

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD-SPW

   Plasmid

   #190628

   Purpose

   Bacterial expression of genetically encoded protease indicator SPW

   Depositor

   Robert Campbell

   Article

   Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

   Insert

   SPW
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMD19T-psba1-Ppsba2-dCas9-SpR

   Plasmid

   #73220

   Purpose

   Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

   Depositor

   Paul Hudson

   Article

   Yao et al ACS Synth Biol. 2015 Dec 28.

   Insert

   dCas9 from S. pyogenes
   

   Use

   Tags

   c-myc

   Expression

   Bacterial

   Mutation

   Silent mutation at bp 1341 A->C to remove an E…

   Promoter

   PpsbA2

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. BC-A1-004

   Plasmid

   #78691

   Purpose

   A strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BC-A1-003

   Plasmid

   #78690

   Purpose

   A strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKDsgRNA-ldhA

   Plasmid

   #102288

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ldhA.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   ldhA gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. BC-A1-013

    Plasmid

    #78701

    Purpose

    A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKDsgRNA-rpsL

    Plasmid

    #89953

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    rpsL gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBP-pET-RBS

    Plasmid

    #72981

    Purpose

    PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    pET RBS derived from pET vectors
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA-SCW

    Plasmid

    #190633

    Purpose

    Mammalian expression of genetically encoded calcium indicator SCW

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCW
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBAD-SKW

    Plasmid

    #190631

    Purpose

    Bacterial expression of genetically encoded potassium indicator SKW

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SKW
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-SCW

    Plasmid

    #190626

    Purpose

    Bacterial expression of genetically encoded calcium indicator SCW

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCW
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Pr-deCFP-MGapt

    Plasmid

    #67740

    Purpose

    OR2-OR1-Pr promoter expressing deCFP-MG aptamer fusion

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deCFP-MGapt-T500
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr

    Available Since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Pr1-T7RNAP

    Plasmid

    #67738

    Purpose

    OR2-OR1-Pr1 promoter expressing T7 RNAP

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-T7RNAP-T500
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr1

    Available Since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. TrkA-mCherry-CRY2

    Plasmid

    #106167

    Purpose

    mammalian expression of full length TrkA fused to mCherry and CRY2PHR (aa 1-498)

    Depositor

    Bianxiao Cui

    Article

    Duan et al ACS Synth Biol. 2018 Jul 20;7(7):1685-16

    Insert

    TrkA mCherry CRY2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. S3_002_026_096

    Plasmid

    #134403

    Purpose

    pYTK096-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    NLS_FdeR-N
    

    Use

    Tags

    None and none

    Expression

    Yeast

    Mutation

    None and none

    Promoter

    pGPM1 and pRPL18B

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BC-A1-005

    Plasmid

    #78692

    Purpose

    A weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CTAG_linker-eGFP

    Plasmid

    #136098

    Purpose

    FP for C-term fusion with CDS12 without linker

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_AlaSerGly10xAlaGlySer_eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CTAG_linker-mTurquoise2

    Plasmid

    #136100

    Purpose

    FP for C-term fusion with CDS12 without linker

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_AlaSerGly10xAlaGlySer_mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. CDS_GUS

    Plasmid

    #136084

    Purpose

    For histochemical _-glucuronidase activity assays. This GUS CDS does not have an intron.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS_GUS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. CDS12_eGFP-linker

    Plasmid

    #136086

    Purpose

    FP for N-term fusion with a CTAG without linker

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS12_eGFP(noStop)-SerGly10xAlaSer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CDS12_mScarletI-linker

    Plasmid

    #136088

    Purpose

    FP for N-term fusion with a CTAG without linker

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS12_mScarletI(noStop)-SerGly10xAlaSer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. CDS12_mScarlet-I

    Plasmid

    #136087

    Purpose

    FP for N-term fusion with a CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS12_mScarletI(noStop)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. acta 2

    Plasmid

    #78711

    Purpose

    zebrafish acta2 3-UTR–specific probe

    Depositor

    Sarah Childs

    Article

    Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

    Insert

    acta2 (acta2 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    contains bp 1,243–1,591

    Promoter

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pET11a-DAAT-V33G/T242G

    Plasmid

    #159278

    Purpose

    Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan

    Depositor

    Roberto Chica

    Article

    Parmeggiani et al ACS Catalysis 9, 3482–3486.

    Insert

    Engineered d-alanine aminotransferase
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Valine 33 and threonine 242 have been mutated to …

    Promoter

    T7

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDGB1a2R+pNOS_Red-F_NOSt

    Plasmid

    #170888

    Purpose

    GoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Reverse orientation

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    Red-F
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    p.S286Y Red mutant

    Promoter

    pNOS

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pMC8b-XI3up

    Plasmid

    #176170

    Purpose

    yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-3

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    5' homology for integration site XI-3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge