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CMV-Kir2.1-pA-CMV-mRFP-pA(AV39)

Plasmid

#117856

Purpose

This plasmid expresses Kir2.1 along with fluorescent marker RFP under the control of CMV promoter. The vector also supports the construction of adenovirus using ADeasy system.

Depositor

C. Ron Yu

Article

Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCHNFI-X2

Plasmid

#31402

Depositor

Richard Gronostajski

Article

Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-

Insert

Nuclear Factor IX isoform 2 (Nfix Mouse)

Use

Tags

NA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-SKM

Plasmid

#136551

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Sox2, Klf4, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Sox2-T2A-Klf4-E2A-cMyc (Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-cFos-mCh

Plasmid

#193066

Purpose

constitutive expression of mouse cFos fused to mCherry in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

cfos-mCh

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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p175 Grg-1s HA

Plasmid

#11064

Depositor

Ramesh Shivdasani

Article

Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

Insert

Grg1-S (Tle1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Esrrb

Plasmid

#71152

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Esrrb

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Estrogen related receptor, beta (Esrrb Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-sgEp300#1/Cre

Plasmid

#173591

Purpose

Expresses a Ep300-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Ep300 (Ep300 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pWZL blast HA goosecoid

Plasmid

#14096

Depositor

Bob Weinberg

Article

Hartwell et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

Insert

goosecoid (Gsc Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pRK5-mFzd8-1D4

Plasmid

#42270

Depositor

Jeremy Nathans, Chris Garcia

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 8 (Fzd8 Mouse)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
Anti-SAP97 [K64/15R]

Plasmid

#128624

Purpose

Mammalian Expression Plasmid of anti-SAP97 (Rat). Derived from hybridoma K64/15.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SAP97 (Rat) recombinant mouse monoclonal antibody (Dlg1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pBa-FRB-3myc-Rab5a

Plasmid

#64209

Purpose

Rab5a with N-terminal FRB and triple myc tags

Depositor

Marvin Bentley, Gary Banker

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Rab5a (Rab5a Mouse)

Use

Tags

3myc and FRB

Expression

Mammalian

Mutation

Promoter

chicken Beta Actin

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pMIY-Arg1

Plasmid

#34580

Depositor

Peter Murray

Insert

Arg1 (Arg1 Mouse)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only
pLJM5-mSHMT1

Plasmid

#83904

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Serine hydroxymethyltransferase 1 (Shmt1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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mVenus-MCHR1-N-10

Plasmid

#56333

Purpose

Localization: Cilium, Excitation: 515, Emission: 528

Depositor

Michael Davidson

Insert

MCHR1

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE

Plasmid

#111393

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
GCaMP6f

Use

AAV

Tags

6xHis, Myc, T7, and Xpress

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAL130

Plasmid

#22475

Purpose

Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF3NT (PIF3 Mustard Weed, Mouse)

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GCaMP6f

Plasmid

#166022

Purpose

Expresses GCaMP6f in CaMKII+ cells

Depositor

Xue Han

Article

Keaveney et al J Neurosci. 2020 Dec 9;40(50):9576-9588.

Insert

GCaMP6f

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-Mfn1-T562A

Plasmid

#191976

Purpose

To produce mutants mimicking dephosphorylated Mfn1

Depositor

Luca Scorrano

Article

Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

Insert

Mfn1 T562A (Mfn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

T562A

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-Mfn1-T562D

Plasmid

#191977

Purpose

To produce mutants mimicking phosphorylated Mfn1

Depositor

Luca Scorrano

Article

Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

Insert

Mfn1 T562D (Mfn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

T562D

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R]

Plasmid

#177486

Purpose

Mammalian Expression Plasmid of anti-Slo1/BKAlpha maxi-K+ channel (Mouse). Derived from hybridoma L6/60.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Slo1/BKAlpha maxi-K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnma1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-ANO3/TMEM16C [N454/91R]

Plasmid

#206587

Purpose

Mammalian Expression Plasmid of anti-ANO3/TMEM16C (Mouse). Derived from hybridoma N454/91.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-ANO3/TMEM16C (Mus musculus) recombinant Mouse monoclonal antibody (Ano3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-GST-Presc-mAkt1 (wt)

Plasmid

#48805

Purpose

expresses wt Akt1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

Akt1 (Akt1 Mouse)

Use

Tags

HA-GST-Presc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only
Anti-Beta4-spectrin [N393/76R]

Plasmid

#177542

Purpose

Mammalian Expression Plasmid of anti-Beta4-spectrin (Human). Derived from hybridoma N393/76.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Beta4-spectrin (Homo sapiens) recombinant mouse monoclonal antibody (SPTBN4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV14/3xFLAG-Pax3

Plasmid

#25427

Depositor

Jonathan Epstein

Insert

Pax3 (Pax3 Mouse)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 25, 2010

Availability

Academic Institutions and Nonprofits only
SPOP-V5 (BTB+BACK)

Plasmid

#169723

Purpose

Mammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

L186D, L190D, L193D, I217K, Y353E

Promoter

CMV

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSuper mouse Drosha1

Plasmid

#14780

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 Apr 1. ():.

Insert

Drosha shRNA (Drosha Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pcdna3mciap1 mut

Plasmid

#11476

Depositor

Jon Ashwell

Article

Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56.

Insert

mciap1mut (Birc2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

582 to alanine

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-E11-ChR2GFP2x

Plasmid

#153434

Purpose

AAV vector to drive the expression of dChr2-GFP-P2A-GFP in PV cortical interneuronsunder the control of the E11 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

Chr2-GFP-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Eomes

Plasmid

#128825

Purpose

dox-dependent expression of mouse Eomes

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Eomes (Eomes Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Shorter isoform

Promoter

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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Dopey2_pCSdest

Plasmid

#53850

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

Dopey2

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits