We narrowed to 12,157 results for: pcDNA
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Plasmid#163994PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imagingDepositorAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1392E
Plasmid#163993PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imagingDepositorAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1386E
Plasmid#163992PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imagingDepositorAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(E1418K/P1424Q/Y1426S)
Plasmid#155006PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationE1418K/P1424Q/Y1426S; insert codes for EBA175 res…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1426H)
Plasmid#155312PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1426H variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationM1423R/P1424V/Y1426H; insert codes for EBA175 res…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(A1419R/P1424Q/Y1426S)
Plasmid#155007PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationA1419R/P1424Q/Y1426S; insert codes for EBA175 res…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(S1421R/P1424Q/Y1426S)
Plasmid#155008PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationS1421R/P1424Q/Y1426S; insert codes for EBA175 res…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-BAEBL (I1134V/P1135L/Y1136V/F1137V)
Plasmid#154875PurposeExpresses N-terminally GFP-tagged Pf-BAEBL P5-P2 VLVV variant (coding for residues 1041-1210) from pcDNA3.1DepositorInsertPfBAEBL (PF3D7_0506900 P. falciparum)
TagsGFP and signal peptide (residues 1 to 32) from TG…ExpressionMammalianMutationI1134V, P1135L, Y1136V, F1137V; insert codes for …PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424I/Y1425I/Y1426I)
Plasmid#154977PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424A/Y1425I/Y1426I variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424/Y1425I/Y1426I; insert codes for EBA175 resi…PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-eGFP-FRT-T0-RNF168 delta MIU1/MIU2
Plasmid#133980Purposeinducible mammalian expression vector of eGFP tagged RNF168 where both motifs interacting with ubiquitin have been deletedDepositorInsertRNF168 (RNF168 Human)
TagseGFPExpressionMammalianMutationdeletion of MIU1 and MIU2PromoterCMVAvailable SinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
FLAG-MBP1-K295TAG-W340A-S233H-T237C-CAAX.pcDNA3-k
Plasmid#127403PurposeExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
TagsCAAX and FLAGExpressionMammalianMutationAmino acid mutations: K295 to Amber codon, W340A,…PromoterCMVAvailable SinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-E322TAG-K305H-E309H-W340A-CAAX.pcDNA3-k
Plasmid#127407PurposeExpresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
TagsCAAX and FLAGExpressionMammalianMutationAmino acid mutations: E322 to Amber codon, K305H,…PromoterCMVAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-K295TAG-W340A-S233H-T237H-CAAX.pcDNA3-k
Plasmid#127401PurposeExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
TagsCAAX and FLAGExpressionMammalianMutationAmino acid mutations: K295 to Amber codon, W340A,…PromoterCMVAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader GUG-nLuc-3XFLAG
Plasmid#127327PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTRDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader AUG-nLuc-3XFLAG
Plasmid#127326PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTRDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-vsv-Incenp d543-746-GFP
Plasmid#108503Purposeinducible expression of vsv-INCENP d543-746-EGFPDepositorInsertINCENP d543-746 (INCENP Human)
TagsEGFP and VSVExpressionMammalianMutationdelta alpha helixPromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His
Plasmid#83457PurposeHuman PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) (632)DepositorInsertPolycystin-1 (PKD1 Human)
Tags6xHis and MycExpressionMammalianMutationAmino-acids 4127-4303Available SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP
Plasmid#65846PurposeMammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)DepositorAvailable SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP
Plasmid#65847PurposeMammalian expression of the mutated N terminal part (R5D-K5D N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)DepositorAvailable SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase delta 55/Non-Cleavable
Plasmid#59827PurposeAllows the integration of myc Separase delta 55/Non-Cleavable in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
TagsMycExpressionMammalianMutationdelta 55/Non-Cleavable (E1483R, R1486E, E1503R, R…Promoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase delta 1278-1291
Plasmid#59830PurposeAllows the integration of myc Separase delta 1278-1291 in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
TagsMycExpressionMammalianMutationdelta 1278-1291Promoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(471-707)(C478S, C667S and C699T)
Plasmid#52129Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJnk interacting protien (Jip1b)(471-707)(M1+2+6) (Mapk8ip1 Mouse)
TagsT7ExpressionMammalianMutationdeleted amino acids 1-470 and changed C478S, C667…Available SinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(471-707)(C478S, C643S and C696T)
Plasmid#52130Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJnk interacting protein Jip1b(471-707)(M1+3+6) (Mapk8ip1 Mouse)
TagsT7ExpressionMammalianMutationdeleted amino acids 1-470 and changed C478S, C643…Available SinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(471-707)(C478S, C631S and C699T)
Plasmid#52131Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJnk interacting protein (Jip1b)(471-707)(M1+4+6) (Mapk8ip1 Mouse)
TagsT7ExpressionMammalianMutationdeleted amino acids 1-470 and changed C478S, C631…Available SinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 P676C I730C (NT809)
Plasmid#50866PurposeExpresses human NKCC1 P676C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationP676C I730C in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 N680C A734C (NT816)
Plasmid#50870PurposeExpresses human NKCC1 N680C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationN680C A734C in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 P676C A734C (NT810)
Plasmid#50867PurposeExpresses human NKCC1 P676C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationP676C A734C in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 A675C A734C (NT841)
Plasmid#50864PurposeExpresses human NKCC1 A675C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationA675C A734C in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 S679C I730C (NT838)
Plasmid#50869PurposeExpresses human NKCC1 S679C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationS679C I730C in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 I674C I730C (NT835)
Plasmid#50863PurposeExpresses human NKCC1 I674C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationI674C I730C in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 I677C A734C (NT813)
Plasmid#50868PurposeExpresses human NKCC1 I677C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationI677C A734C in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 intracellular-cysteineless (NT855)
Plasmid#49080PurposeExpresses human NKCC1 mutant lacking cysteine residues within intracellular loops and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and mVenusExpressionMammalianMutationC791S, C820S, C1052S in hNKCC1 in NT17PromoterCMVAvailable SinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 ECL4 deletion (NT865)
Plasmid#49070PurposeExpresses human NKCC1 truncation mutant lacking extracellular loop #4 (aa562-582) and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and mVenusExpressionMammalianMutationECL4 deletion of amino acids 562-582 in hNKCC1 in…PromoterCMVAvailable SinceNov. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S,C724V (NT501)
Plasmid#49073PurposeExpresses human NKCC1 C723S and C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and mVenusExpressionMammalianMutationC723S,C724V in hNKCC1 in NT17PromoterCMVAvailable SinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 N-terminal deletion (NT53)
Plasmid#49062PurposeExpresses human NKCC1 truncation mutant (aa13-222) with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and mVenusExpressionMammalianMutationN-terminal deletion of amino acids 13-222 in hNKC…PromoterCMVAvailable SinceNov. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-645, Δ368-523)/I346A/HA tag/pcDNA3.1
Plasmid#111460PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
TagsHa TagExpressionMammalianMutationKv7.4 (1-645, Δ368-523)/I346A, C643AAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-645, Δ368-523)/I346D/HA tag/pcDNA3.1
Plasmid#111461PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
TagsHa TagExpressionMammalianMutationKv7.4 (1-645, Δ368-523)/I346D, C643AAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT_HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2
Plasmid#226514PurposeMamalian cell expression of nuclear HaloTag7-SNAP-tag2 and nuclear mTurquoise2DepositorInsertHaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2
ExpressionMammalianPromoterCMVAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato
Plasmid#138610PurposeExpress TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backboneDepositorInsertsHyperactive AID
T7 RNA Polymerase
tdTomato
ExpressionMammalianAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-b2AR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry
Plasmid#113610PurposePPI reporterDepositorInsertbeta 2 adrenergic receptor
ExpressionMammalianAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_CalR-HaloTag7-P30-SNAP-tag-KDEL
Plasmid#182012PurposeCMV overexpression of HaloTag7 and SNAP-tag fusion in the endoplasmic reticulum of mammalian cell linesDepositorInsertCalR-HaloTag7-P30-SNAP-tag-KDEL
ExpressionMammalianPromoterCMVAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-muOR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry
Plasmid#113611PurposePPI reporterDepositorInsertmu opioid receptor
ExpressionMammalianAvailable SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)
Plasmid#140893PurposeExpresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian celDepositorTagsmCherryExpressionMammalianMutationchanged 223L to F in Dr-VSP (zebrafish TPTE), cha…Available SinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO(Neo)-Venus-Flag-WT wobbled cdc20 (1991)
Plasmid#61848Purposefor making stable RPE cells expressing WT cdc20DepositorAvailable SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST
Plasmid#127332PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRESDepositorInsertnanoLuciferase
Tags3xFLAG and CL1/PESTExpressionMammalianPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-S1P1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry
Plasmid#113612PurposePPI reporterDepositorInsertS1P1
ExpressionMammalianAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-PAR1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry
Plasmid#113608PurposePPI reporterDepositorInsertPAR1
ExpressionMammalianAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-PAR2-GFP11-T2A-GFP10-bArrestin1-T2A mCherry
Plasmid#113609PurposePPI reporterDepositorInsertPAR2
ExpressionMammalianAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 TEV-GFP-FLAG LIC cloning vector (6LD)
Plasmid#166835PurposeModified LIC 6D backbone with a C-terminal TEV-GFP-FLAG tag. Derived from Scott Gradia's pcDNA3.1 LIC 6D by Liron David.DepositorTypeEmpty backboneExpressionMammalianAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-110)Cy-
Plasmid#169338PurposeExpression of the LT-Fucci(CA) in mammalian cellsDepositorTagsHaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-110)Cy…ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only