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pAAV-CAG-iGlucoSnFR-TS

Plasmid

#127952

Purpose

Expresses the iGlucoSnFR-TS biosensor for intracellular [glucose], with a fluorescence-lifetime readout, in mammalian cells

Depositor

Gary Yellen

Article

Diaz-Garcia et al J Neurosci Res. 2019 May 20. doi: 10.100

Insert

iGlucoSnFR-TS

Use

AAV

Tags

His6 and Xpress epitope

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG FUCCI

Plasmid

#136934

Purpose

donor plasmid for constitutive FUCCI expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Use

Synthetic Biology

Tags

Clover and mKO2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-shRab27a

Plasmid

#120930

Purpose

conditionally knockdown Rab27a in mouse cell lines

Depositor

Mien-Chie Hung

Article

Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

Insert

shRab27a (Rab27a Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE hSPC-eGFP-W

Plasmid

#36450

Depositor

Darrell Kotton

Article

Longmire et al Cell Stem Cell. 2012 Apr 6;10(4):398-411

Inserts

human SP-C promoter
eGFP (eGFP )

Use

Lentiviral

Tags

Expression

Mutation

Promoter

N/A and human SP-C promoter

Available since

May 11, 2012

Availability

Academic Institutions and Nonprofits only

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SWEET11:SWEET11-2A-GFP-GUS

Plasmid

#172484

Purpose

Translational fusion of SWEET11 with a P2A self cleaving peptide sequence

Depositor

Wolf Frommer

Article

Kim et al Plant Cell. 2021 May 5;33(3):511-530. do

Insert

SWEET11 (AT3G48740 Mustard Weed)

Use

Tags

GFP, GUS

Expression

Plant

Mutation

Promoter

SWEET11

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMP71-super-IL-2

Plasmid

#174599

Purpose

Expresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia Lab

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

extracellular domain of huIL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21-Q76L (CA)

Plasmid

#83422

Purpose

Mouse Rab21 mutant that has defects in GTP hydrolysis (GTP-locked, constitutively active). Generated from pEGFP-Rab21 by site-directed mutagenesis.

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Residue 76 from Q to L (Q76L: CAG -> CTG)

Promoter

CMV

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21-T31N (DN)

Plasmid

#83423

Purpose

Mouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Residue 31 from T to N (T31N: ACG -> AAC)

Promoter

CMV

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSEVA331Bb

Plasmid

#78269

Purpose

pSEVA331 with Biobrick multiple cloning site

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pEND-193_Cacnes_HS

Plasmid

#225622

Purpose

C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)

Depositor

Marc Güell

Article

Guillermo et al Cell Systems, 2025

Insert

HspR

Use

Synthetic Biology

Tags

pFAST-6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-192_Cacnes_HS

Plasmid

#225621

Purpose

C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS09160)+RBS_1+HspR(PPA_RS10230).

Depositor

Marc Güell

Article

Guillermo et al Cell Systems, 2025

Insert

HspR

Use

Synthetic Biology

Tags

pFAST-6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sct-p5-b2m-HLA-A0101

Plasmid

#196512

Purpose

Express single-chain peptide-MHC (CMV p5 epitope on HLA-A0101)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a CMV peptide, b2m and HLA A0101

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C

Promoter

CMV

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-EPG

Plasmid

#117978

Purpose

Express EPG in mamalian cells

Depositor

Assaf Gilad

Article

Krishnan et al Sci Rep. 2018 Jun 8;8(1):8764. doi: 10.1

Insert

Electromagnetic perceptive gene

Use

Tags

HIS and v5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 barr2 HA

Plasmid

#14692

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

beta-arrestin 2 (Arrb2 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-MXD1

Plasmid

#178443

Purpose

doxycycline-inducible expression of Human MXD1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

MXD1 (MXD1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Klf4

Plasmid

#20322

Depositor

Rudolf Jaenisch

Article

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

Klf4 (Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3 barr1 HA

Plasmid

#14693

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

beta arrestin 1 (Arrb1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
Flag-Rabin8

Plasmid

#118070

Purpose

Expresses Flag-tagged human Rabin8 in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

human Rabin8 (RAB3IP Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLux01

Plasmid

#78281

Purpose

mRFP1 expressed behind an AHL-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

AHL inducible mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-DN-REST

Plasmid

#61533

Purpose

Expresses DN-REST in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

REST (REST Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

P73, Dominant negative REST (contains the Zf DNA-…

Promoter

Available since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only

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