We narrowed to 31,552 results for: Eng;

1. pBTK601

   Plasmid

   #110607

   Purpose

   BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   Cas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ292 (AaCas12b)-D570A-TV-MS2-TV

   Plasmid

   #136381

   Purpose

   CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b (D570A)-TV-T2A-MCP-TV
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D570A

   Promoter

   Available since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDIRECT_25I

   Plasmid

   #91146

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTK-EdnrbE2-Sox10-FLAG-tev-AVI

   Plasmid

   #127775

   Purpose

   Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Sox10 in frame with a Flag-tag and Avi-tag

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   sox10 (SOX10 Chicken)
   

   Use

   Enhancer reporter construct

   Tags

   Avi

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDIRECT_22D

   Plasmid

   #91136

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNTPTP alpha (S180A/S204A)

   Plasmid

   #17700

   Depositor

   Xinmin Zheng

   Article

   Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

   Insert

   PTPalpha S180A S204A (PTPRA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S180A / S204A

   Promoter

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pDIRECT_21D

   Plasmid

   #91131

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NG1237 pCI-TPI-PTC160-4H

   Plasmid

   #65804

   Purpose

   NMD reporter mRNA

   Depositor

   Niels Gehring

   Article

   Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

   Insert

   TPI (TPI1 Human)
   

   Use

   Tags

   4H (4 copies of short beta-globin 3'UTR for …

   Expression

   Mammalian

   Mutation

   premature stop codon at 160

   Promoter

   CMV

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJY-RpABE-FT_INF

   Plasmid

   #112874

   Purpose

   binary vector for INF (Induced Neo-Functionalization) of FT in Arabidopsis

   Depositor

   Jin-Soo Kim, Jae-Young Yun

   Article

   Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

   Inserts

   RPS5A promoter
   ABE7.10
   U6-sgRNA targeting FT
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. UASgal-A9-Pleum

    Plasmid

    #60343

    Purpose

    Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pMXs-1003

    Plasmid

    #31712

    Depositor

    Duanqing Pei

    Article

    Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

    Insert

    miR-367 (Mir367 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

12. CNC3 (CASANOVA-C3)

    Plasmid

    #137191

    Purpose

    Expresses CNC3 (CASANOVA-C3), a blue light dependent NmeCas9 inhibitory protein

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

    Insert

    CNC3 (CASANOVA-C3)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET-pr-mEos4a (mEosFP4a-V69T)

    Plasmid

    #99225

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEos4a (mEos4a-V69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos4a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V69T for primed conversion applications

    Promoter

    T7

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. PatoG-OG241

    Plasmid

    #72839

    Purpose

    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone.

    Depositor

    Chris Barnes

    Article

    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

    Insert

    PatoG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PatoG

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. NG0911 pCI-TPI-SMG5(short)-4H

    Plasmid

    #65805

    Purpose

    NMD reporter mRNA

    Depositor

    Niels Gehring

    Article

    Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

    Insert

    TPI (TPI1 Human)
    

    Use

    Tags

    4H (4 copies of short beta-globin 3'UTR for …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSmBit-CHIP-K30A

    Plasmid

    #236066

    Purpose

    SmBit-CHIP expression with K30A substitution

    Depositor

    Jonathan Schisler

    Article

    Stewart et al bioRxiv 2025.02.17.637997

    Insert

    SmBit-CHIP-K30A (STUB1 Human)
    

    Use

    Luciferase

    Tags

    SmBit

    Expression

    Mutation

    K30A

    Promoter

    HSV-TK

    Available since

    Sept. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. FUS LC 8Y->S

    Plasmid

    #226606

    Purpose

    8 tyrosines to serine

    Depositor

    Nicolas Fawzi

    Article

    Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

    Insert

    FUS LC 8Y->S (FUS Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    8 tyrosines to serine

    Promoter

    T7

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pILGFPEasy9R

    Plasmid

    #218291

    Purpose

    Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.

    Depositor

    Claudia Vickers

    Article

    McDonnell et al bioRxiv 2024.03.26.586389

    Insert

    pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-Nova1-GFP

    Plasmid

    #217033

    Purpose

    Overexpression of Nova in DRG neurons promotes the "mature" splicing pattern observed in CNS neurons

    Depositor

    Chaolin Zhang

    Article

    Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

    Insert

    Nova1 (Nova1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3-MTS-G3635A-R16-Q2L7-T2176C-UGI-Puro

    Plasmid

    #209648

    Purpose

    To install m.G3635 mutation on mtDNA

    Depositor

    Bin Shen

    Article

    Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

    Insert

    R16-Q2L7-T2176C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge