We narrowed to 18,262 results for: Met;

1. pDS221

   Plasmid

   #34981

   Purpose

   Expresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   ePDZb1–mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE102

   Plasmid

   #59397

   Purpose

   MethyloBrick expression vector, mxaF promoter; TcR

   Depositor

   Tobias Erb

   Article

   Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PmxaF

   Available since

   Dec. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Yellow Cameleon-Nano15/pcDNA3

   Plasmid

   #51961

   Purpose

   mammalian expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano15
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pTriEx-mCherry-LOV2 I427T

   Plasmid

   #81035

   Purpose

   Mammalian expression of LOV I427T, a mutant with super short relaxation time

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   LOV I427T
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   I427T

   Promoter

   Available since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGal4AD-CIBN

   Plasmid

   #28246

   Purpose

   Expresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activation

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CIB1 (1-171aa)
   

   Use

   Tags

   Gal4AD

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. rASAP

   Plasmid

   #178205

   Purpose

   Ratiometric version of the genetically encoded voltage indicator ASAP2s-I67T-Q397R.

   Depositor

   Stefan Heinemann

   Article

   Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

   Insert

   rASAP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmScarlet-H_NES_C1

   Plasmid

   #85061

   Purpose

   In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. scFv-IFP2.0N

   Plasmid

   #215746

   Purpose

   Expresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   IFP2.0N
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

   Plasmid

   #83946

   Purpose

   Cas9-carrying vector with kanMX marker

   Depositor

   Irina Borodina

   Article

   Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-NLS-PhoCl-mCherry

    Plasmid

    #87691

    Purpose

    Express NLS-PhoCl-mCherry in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NLS-PhoCl-mCherry
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-CT3G-ERP2-MG

    Plasmid

    #175501

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available since

    Oct. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMV-Cas9C-VPR

    Plasmid

    #80933

    Purpose

    Expresses Cas9C-VPR in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C-VPR
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRIPZ-NLS-PTEN

    Plasmid

    #206984

    Purpose

    Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with NLS

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. mEos4a-N1

    Plasmid

    #54811

    Purpose

    Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson, Loren Looger

    Article

    Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Type

    Empty backbone

    Use

    Tags

    mEos4a

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCFJ910

    Plasmid

    #44481

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, NeoR, MCS
    

    Use

    Multiple cloning site vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. AB.pCCL.sin.cPPT.GFP.miR-126-3p.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85908

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-126-3p Sensor (MIR126 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ii-Str_ManII-SBP-mCherry

    Plasmid

    #65256

    Purpose

    synchronize trafficking of ManII from the ER

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ii-Streptavidin and truncated Mannosidase II
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEB2-DsRed-Express2

    Plasmid

    #103999

    Purpose

    Plasmid encoding DsRed-Express2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    DsRed-Express2
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF_dCas9-VP64

    Plasmid

    #68417

    Purpose

    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and VP64

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    human EF1[alpha]

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ii-Str_ManII-SBP-EGFP

    Plasmid

    #65255

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ii-Streptavidin and truncated Mannosidase II
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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