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pBXPHM3-PCFT_NB

Plasmid

#165415

Purpose

Expression in E.coli of N terminally MBP tagged anti-GgPCFT nano body

Depositor

Simon Newstead

Article

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT_NB (SLC46A1 Chicken, Llama)

Tags

His10-MBP-3C protease site

Expression

Bacterial

Promoter

arabinose

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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Axin1_HA in pRK5

Plasmid

#154220

Purpose

C-terminal HA-tagged AXIN1 cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

AXIN1 (AXIN1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-API5

Plasmid

#157655

Purpose

Expresses API5 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Tags

His tag

Expression

Bacterial

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-Rpp40-C1

Plasmid

#134549

Purpose

Expresses YFP-Rpp40 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp40 (RPP40 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-Rpp25-C1

Plasmid

#134544

Purpose

Expresses YFP-Rpp25 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp25 (RPP25 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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CFP-Rpp29-C1

Plasmid

#134546

Purpose

Expresses CFP-Rpp29 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp29 (POP4 Human)

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pABM-D4-miR30-CypA

Plasmid

#132934

Purpose

Lentivector for CypA knockdown and blasticidin resistance

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

CypA shRNA

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSDMA66

Plasmid

#128355

Purpose

gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.

Depositor

James Fraser

Article

Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

Insert

Noureothricin (NAT)

Use

Unspecified

Promoter

ACT1

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS tat

Plasmid

#101326

Purpose

Encodes codon optimized NL4-3 tat

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 tat

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCF730

Plasmid

#121668

Purpose

EF1a-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Promoter

EF-1a core promoter

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCF713

Plasmid

#121665

Purpose

U6-sgRNA EF1a-ProCas9-FlaviS6-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pXylA-RgTALsyn

Plasmid

#100948

Purpose

Expression of Tyrosine Ammonia Lyase from constitutive XylA promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

XylA (Constitutive)

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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integrin alpha9 / alpha4 pcDNA1 neo

Plasmid

#13587

Depositor

Dean Sheppard

Article

Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

Insert

integrin alpha 9 / alpha 4 (ITGA9 Human)

Expression

Mammalian

Mutation

Integrin alpha 9 chimera containing the cytoplasm…

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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integrin alpha9 / alpha5 pcDNA1 neo

Plasmid

#13588

Depositor

Dean Sheppard

Article

Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

Insert

integrin alpha 9 / alpha 5 (ITGA9 Human)

Expression

Mammalian

Mutation

Integrin alpha 9 chimera containing the cytoplasm…

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pGL2209

Plasmid

#137050

Purpose

Drug Resistance pDONR221 PUR

Depositor

Jeremy Mottram

Article

Duncan et al Methods Mol Biol. 2019;1971:211-224. doi

Insert

puromycin N-acetyl-transferase

Use

Leishmania expression gateway vector

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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yCp50-PRP5

Plasmid

#111303

Purpose

Prp5 in yCp50 plasmid

Depositor

Christine Guthrie

Article

Noble et al Genetics. 1996 May;143(1):67-80.

Insert

PRP5

Expression

Yeast

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pTALEN-STAR-T

Plasmid

#87522

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALEN-STAR-A

Plasmid

#87523

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALEN-STAR-C

Plasmid

#87524

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALEN-STAR-G

Plasmid

#87525

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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