161,108 results

1. pGreen_dualluc_3'UTR_sensor

   Plasmid

   #55206

   Purpose

   Dual-luciferase reporter for microRNA targeting in plants

   Depositor

   Michael Axtell

   Article

   Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

   Inserts

   Renilla luciferase
   Firefly luciferase
   

   Use

   Luciferase; Plant transient expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-Puro_hCOL4A2-Myc-His-WPRE

   Plasmid

   #198080

   Purpose

   Express hCOL4A2 in mammalian cells

   Depositor

   Jeffrey Miner

   Insert

   COL4A2 (COL4A2 Human)
   

   Use

   Tags

   TEV-Myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, beta-globin/IgG chimeric intron

   Available since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF2432

   Plasmid

   #144026

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ARID1A (ARID1A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG87 FAST

   Plasmid

   #130719

   Purpose

   Expresses His-tagged FAST (also called YFAST) in bacteria

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   FAST
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. CD34-bio-His

   Plasmid

   #51647

   Purpose

   Expresses full-length Hematopoietic progenitor cell antigen CD34 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   CD34 (CD34 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJAT7

   Plasmid

   #204292

   Purpose

   For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-DsRed
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   3XP3

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC1 Grx1-roCherry-mito

   Plasmid

   #141069

   Purpose

   Fluorescent biosensor for 2GSH/GSSG ratio with mitochondrial localization

   Depositor

   Dmitry Bilan

   Article

   Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

   Insert

   Grx1-roCherry
   

   Use

   Tags

   dMTS

   Expression

   Mammalian

   Mutation

   Changed Alanine 150 to Cysteine, inserted Threoni…

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGreenFire_blastocidin

   Plasmid

   #174103

   Purpose

   pGreenFire MPRA plasmid for cloning

   Depositor

   Paul Khavari

   Article

   Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLNCX2-mCherry-CHMP2A

   Plasmid

   #115330

   Purpose

   expresses mCherry-CHMP2A in mammalian cells

   Depositor

   Sanford Simon

   Article

   Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

   Insert

   CHMP2A (CHMP2A Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSaGuide

    Plasmid

    #64710

    Purpose

    Cloning and expression of Staphylococcus aureus guide RNA

    Depositor

    Kiran Musunuru

    Insert

    Staphylococcus aureus guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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