We narrowed to 34,848 results for: CaS;

1. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA

   Plasmid

   #136654

   Purpose

   Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD9:CNRZ1066 chimera
   sgRNA for St1Cas9
   APOBEC-1 (APOBEC1 Human)
   UGI
   HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-T3-HypaCAS9-pA

   Plasmid

   #131467

   Purpose

   Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.

   Insert

   Codon optimized HypaCas9
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-Blast_HP1beta_gRNA

   Plasmid

   #127908

   Purpose

   WT Cas9 Vector with Blasticidin Selection Marker targeting the 5' end of the human HP1beta gene

   Depositor

   Mark Groudine

   Insert

   gRNA for Human 5' HP1beta
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. KA2963_eSpCas9-PGKHygdtkCh

   Plasmid

   #124205

   Purpose

   Expression of eSpCas9(1.1) and sgRNA

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJUMP18-dCas9_O

   Plasmid

   #127028

   Purpose

   Basic Part O- ORF; Catalytically dead mutant of the Cas9 from Streptococcus pyogenes.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Insert

   Part dCas9_O
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   July 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHMGWA-Cas1

   Plasmid

   #122285

   Purpose

   Cas1 cloned in pHMGWA

   Depositor

   Jennifer Doudna

   Article

   Nunez et al Nat Struct Mol Biol. 2014 Jun;21(6):528-

   Insert

   Eco Cas1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. BPK2013-BsCas12b

   Plasmid

   #121952

   Purpose

   E. coli expression, protein purification

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   BsCas12b
   

   Use

   Tags

   10xHis tag and 3xHA tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9-J23113

   Plasmid

   #119701

   Purpose

   Expression of Cas9 with a weak promoter

   Depositor

   Chong Zhang

   Article

   Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-CMV-TO-Casp3te-tv-cx

   Plasmid

   #116056

   Purpose

   Reporter plasmid for measuring protease activity

   Depositor

   Michael Elowitz

   Article

   Gao et al Science. 2018 Sep 21;361(6408):1252-1258

   Insert

   Casp3te-tv-cx
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSD_SpCas9MT3-FKBP_SL

    Plasmid

    #107302

    Purpose

    Expresses R1335K mutant SpCas9 fused to FKBP in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    FKBP

    Expression

    Mammalian

    Mutation

    R1335K and fused to FKBP

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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