166,034 results

1. AAV pCAG-FLEX-EGFP-WPRE

   Plasmid

   #51502

   Purpose

   Can be used to generate AAV virus that will express EGFP in the presence of Cre

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EGFP
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GFP-P4M-SidMx2

   Plasmid

   #51472

   Purpose

   Biosensor for PtdIns4P

   Depositor

   Tamas Balla

   Article

   Hammond et al J Cell Biol. 2014 Apr 7.

   Inserts

   P4M-SidM(546-647)
   P4M-SidM(546-647)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGAL4-DBD-MCS

   Plasmid

   #145245

   Purpose

   Backbone vector expressing GAL4 DNA-binding domain followed by MCS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTXB1-nbMmIgG2a-Tn5

   Plasmid

   #184288

   Purpose

   Anti-mouse IgG2a Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

   Depositor

   New York Genome Center, Ivan Raimondi

   Article

   Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

   Insert

   nbMmIgG2a-Tn5
   

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. V48 ELP

   Plasmid

   #68395

   Purpose

   Elastin-like polypeptide (VPGxG) with 48 repeats of valine guest residue

   Depositor

   Andrew MacKay

   Article

   Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

   Insert

   V48 ELP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-15F11-HA-mEGFP

   Plasmid

   #129590

   Purpose

   The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

   Depositor

   Tim Stasevich

   Article

   Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

   Insert

   Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lenti-dCas9-TurboID-blast

   Plasmid

   #222599

   Purpose

   Express fussed dCas9-TurboID for proximity labeling

   Depositor

   Yarui Diao

   Article

   Sun et al Nat Genet. 2023 Aug;55(8):1324-1335. doi

   Insert

   dCas9-TurboID
   

   Use

   Lentiviral

   Promoter

   EF-1α

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator

   Plasmid

   #168291

   Purpose

   optimized miniCMV (OminiCMV)

   Depositor

   Hui Yang

   Article

   Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

   Insert

   mCherry
   

   Expression

   Mammalian

   Promoter

   miniCMV

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQCXIP-BSR-GFP11

   Plasmid

   #68716

   Purpose

   Split GFP assay

   Depositor

   Yutaka Hata

   Article

   Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

   Insert

   bsr
   

   Use

   Retroviral

   Tags

   FLAG and GFP11

   Expression

   Mammalian

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA

    Plasmid

    #168305

    Purpose

    mediated knock-in of sgRNA precursor

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    SaKKH-EGFP-U6-sgRNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1alpha, CBh

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHS_Nova

    Plasmid

    #239831

    Purpose

    NovaIscB mammalian expression

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    NovaIscB
    

    Tags

    Nucleoplasmin NLS-3xHA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Insertion of REC domain from NbaCas9-1 and swap o…

    Promoter

    CMV

    Available Since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6-sgRNA EF1Alpha-puro-T2A-BFP

    Plasmid

    #60955

    Purpose

    Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

    Depositor

    Jonathan Weissman

    Article

    Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

    Inserts

    sgGFP-NT2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR_pGK_LaG17_synNotch_Gal4VP64

    Plasmid

    #79127

    Purpose

    Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    LaG17_synNotch_Gal4DBDVP64
    

    Use

    Lentiviral

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLX209-neo-active WRN

    Plasmid

    #125788

    Purpose

    constitutive, ectopic expression of human active WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    myc-active WRN (WRN Human)
    

    Tags

    5x myc

    Expression

    Mammalian

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMRXIP 3xFLAG-VMP1

    Plasmid

    #117416

    Purpose

    Expresses VMP1 tagged with 3xFLAG in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    VMP1 (VMP1 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3/F-tractin=mStayGold

    Plasmid

    #212019

    Purpose

    For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    F-tractin
    

    Tags

    mStayGold

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSNAPf-ADRβ2 Control Plasmid

    Plasmid

    #101123

    Purpose

    Expression of SNAP-Tagged β2 Adrenergic Receptor in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    Beta-2-Adrenergic Receptor (ADRB2 Human)
    

    Tags

    SNAP-tag (SNAPf)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pAAV-CAG-NLS-GFP

    Plasmid

    #104061

    Purpose

    AAV expression of nuclear localized GFP with SV40 NLS

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Has Service

    AAV PHP.eB

    Insert

    GFP
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLS-SceI

    Plasmid

    #137725

    Purpose

    Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

    Depositor

    Nadav Ahituv

    Article

    Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

    Plasmid

    #198753

    Purpose

    Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity

    Depositor

    Jacco van Rheenen

    Article

    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

    Insert

    EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Mutation

    See depositor comments below

    Promoter

    EF1a

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge