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We narrowed to 9,660 results for: Gnas

Showing: 7201 - 7250 of 9660 results

  3. pYHY3

    Plasmid
    #216109
    Purpose
    aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.
    Depositor
    Shannon Sirk
    Article
    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:
    Insert
    NanoLuc
    Use
    Synthetic Biology; E. coli-bacteroides shuttle ve…
    Tags
    3xFLAG tag and BT3630 signal peptide
    Expression
    Bacterial
    Mutation
    Promoter
    P1TDP-A21
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216109-plasmid-map-sequence-id-434372

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  6. pHis17_ParM _1

    Plasmid
    #78201
    Purpose
    Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor
    Depositor
    Martin Webb
    Article
    Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8
    Insert
    parM (K33A I27C T174A T175N C287A)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    K33A I27C T174A T175N C287A
    Promoter
    unknown
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    78201-plasmid-map-sequence-id-151697

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  7. pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA

    Plasmid
    #99698
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAV
    Depositor
    George Church
    Type
    Empty backbone
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    99698-plasmid-map-sequence-id-194602

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  12. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Expression
    Mutation
    Promoter
    TgTUB1 and Toxoplasma U6
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

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  17. pCoofy64

    Plasmid
    #122016
    Purpose
    Baculovirus vector for parallel SLIC cloning containing gp67 signal sequence, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    His6-Smt3Star and Multi3: depending on the LP2 pr…
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122016-plasmid-map-sequence-id-243594

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  18. 4xmt-mTurquoise2

    Plasmid
    #98819
    Purpose
    In vivo visualization of mitochondria (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    mts
    Use
    Tags
    mTurquoise2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    98819-plasmid-map-sequence-id-214508

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  19. CMV-EGFP-ssHRP-TM

    Plasmid
    #31156
    Depositor
    Hollis Cline
    Article
    Li et al Front Neural Circuits. 2010 . 4():6.
    Insert
    ssHRP-TM
    Use
    Membrane targeted hrp (mhrp)
    Tags
    Expression
    Mammalian
    Mutation
    includes signal sequence and a transmembrane doma…
    Promoter
    CMV promoter including immediate early promoter/e…
    Available since
    Dec. 14, 2011
    Availability
    Academic Institutions and Nonprofits only

  22. pNM262

    Plasmid
    #128412
    Purpose
    Vanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.
    Depositor
    Christopher Voigt
    Article
    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/
    Insert
    Lysostaphin (epr Staphylococcus simulans)
    Use
    Synthetic Biology
    Tags
    amyE SP
    Expression
    Bacterial
    Mutation
    Promoter
    Pspank(V)
    Available since
    Sept. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    128412-plasmid-map-sequence-id-252487

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  28. pHEA

    Plasmid
    #168297
    Purpose
    Bacterial surface display of polypeptides (nanobodies) in E. coli
    Depositor
    Luis Ángel Fernández
    Article
    Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.
    Insert
    Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
    Use
    Tags
    6xHis, E-tag, and PelB signal peptide
    Expression
    Bacterial
    Mutation
    Promoter
    LacI-Plac
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    168297-plasmid-map-sequence-id-334448

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  36. pSEVA63_Helper

    Plasmid
    #190432
    Purpose
    M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)
    Depositor
    Manish Kushwaha
    Article
    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.
    Insert
    M13 genes: I-XI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    190432-plasmid-map-sequence-id-371816

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  47. LeGO-iG2-wPRE-pA

    Plasmid
    #60489
    Purpose
    eGFP-expressing lentiviral vector with IRES and pA-signal; suitable for use as NRTLV (non-reverse transcribable lentiviral vector)
    Depositor
    Boris Fehse
    Article
    Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103
    Insert
    IRES-eGFP; wPRE; BGH-pA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    60489-plasmid-map-sequence-id-96860

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  48. pAAV-UbC-eGFP-F

    Plasmid
    #71545
    Purpose
    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.
    Depositor
    Pantelis Tsoulfas
    Insert
    eGFP-F
    Use
    AAV
    Tags
    contains 20- amino-acid farnesylation signal from…
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    71545-plasmid-map-sequence-id-132622

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Showing: 7201 - 7250 of 9660 results