We narrowed to 10,981 results for: Vars;

1. pSG5-SUV39H2-FL

   Plasmid

   #89135

   Purpose

   mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase from pSG5, includes 5' and 3' noncoding regions

   Depositor

   Elizabeth Wilson

   Article

   Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

   Insert

   SUV39H2 (SUV39H2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-EF1alpha-dCas9-p300_Blast

   Plasmid

   #192653

   Purpose

   3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   dCas9-p300
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A; H840A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-PIMT

   Plasmid

   #34852

   Depositor

   Bruce Downie

   Article

   Dinkins et al Plant J. 2008 Jul;55(1):1-13.

   Insert

   PIMT (PCMT1 Human)
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pMC037-HisMS2_PLP_pac

   Plasmid

   #128233

   Purpose

   Expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

   Depositor

   Paul Freemont

   Article

   de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

   Inserts

   Maturation Protein
   Coat Protein Dimer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 15, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. LT3GECIR

   Plasmid

   #111178

   Purpose

   Tet-ON miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   T3G

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Dsup pLVX-Puro

   Plasmid

   #180635

   Purpose

   Expresses human codon optimized tardigrade damage suppressing protein Dsup under a CMV promoter; to provide increased radio tolerance

   Depositor

   Christopher Mason

   Article

   Westover et al bioRxiv 2020.11.10.373571

   Insert

   Dsup
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pST-2K

   Plasmid

   #44564

   Purpose

   for ectopic constitutive expression of two mycobacterial proteins in one vector

   Depositor

   Vinay Nandicoori

   Article

   Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

   Type

   Empty backbone

   Use

   E.coli-mycobacteria shuttle vector

   Tags

   Flag tag and His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBad-mCherry-XL (R125K)

   Plasmid

   #188054

   Purpose

   Expresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteria

   Depositor

   Ralph Jimenez

   Article

   Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

   Insert

   mCherry-XL (R125K)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   differs from mCherry by R125K, W143S, I161V, Q163…

   Promoter

   araBAD

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PGK plasmid

   Plasmid

   #169744

   Purpose

   Unregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200406

    Purpose

    Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    MCP-ADAR2dd(E488Q)-NES
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    E488Q (hyperactive)

    Promoter

    CMV

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. SaKKH-ABEmax

    Plasmid

    #119815

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSaCas9 (KKH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFCcGi

    Plasmid

    #59324

    Purpose

    Inducible expression of GFP and constitutive expression of mCherry

    Depositor

    Sophie Helaine, David Holden

    Article

    Figueira et al MBio. 2013 Apr 16;4(2):e00065. doi: 10.1

    Inserts

    mCherry
    EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbad and rpsM

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRDA_078

    Plasmid

    #158582

    Purpose

    Lentiviral expression of BE4max and customizable gRNA

    Depositor

    David Root, John Doench

    Article

    Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 D10A

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDAS12395_PEAR-GFP_2.0-preedited

    Plasmid

    #177185

    Purpose

    PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CamKIIa-moxBFP-WPRE

    Plasmid

    #194976

    Purpose

    AAV vector to drive expression of moxBFP under the control of CamKIIalpha promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    moxBFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2T-CMV-evoCjCas9-TadCDd-BlastR

    Plasmid

    #202558

    Purpose

    Mammalian expression of evoCjCas9 TadCDd

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-TadCDd
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRDA_256

    Plasmid

    #158581

    Purpose

    Lentiviral expression of BE3.9max and customizable gRNA

    Depositor

    David Root, John Doench

    Article

    Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 D10A

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3 mSmoothened WT

    Plasmid

    #126408

    Purpose

    Full length mouse Smoothened cDNA. Expression of Smoothened in mammalian cell lines.

    Depositor

    Stacey Ogden

    Article

    Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

    Insert

    m-Smoothened (Smo Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-ReaChR-citrine

    Plasmid

    #50956

    Purpose

    ReaChR-citrine in lentival transfer vector under human synapsin promoter

    Depositor

    Roger Tsien

    Article

    Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

    Insert

    ReaChR-citrine
    

    Use

    Lentiviral

    Tags

    citrine

    Expression

    Mutation

    Promoter

    human synapsin promoter

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMP2463

    Plasmid

    #107774

    Purpose

    Expression of EGFP

    Depositor

    Herman Spaink

    Article

    Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    pLacZ-EGFP in pBBR1-mcs5
    

    Use

    Tags

    pLacZ-EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    pLacZ

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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