4,178 results

1. pcDNA3-SypHluorin 4x (S4x)

   Plasmid

   #37005

   Depositor

   Yongling Zhu, Stephen Heinemann

   Article

   Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

   Insert

   SypHluorin 4x (Syp Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Four copies of pHluorin inserted at site 184-185

   Promoter

   CMV

   Available Since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe B-Raf V600E

   Plasmid

   #17544

   Depositor

   Channing Der

   Article

   Hao et al Mol Cancer Ther. 2007 Aug . 6(8):2220-9.

   Insert

   B-Raf (BRAF Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   V600E

   Available Since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. Rictor_1 shRNA

   Plasmid

   #1853

   Depositor

   David Sabatini

   Article

   Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

   Insert

   Rictor (RICTOR Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 10, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. STARR-seq luciferase validation vector_ORI_empty

   Plasmid

   #99297

   Purpose

   Luciferase validation vector; empty vector without insert to determine baseline

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Type

   Empty backbone

   Use

   Luciferase; Starr-seq luciferase validation vector

   Expression

   Mammalian

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS513 EF1alpha-cre

   Plasmid

   #11918

   Purpose

   Expresses Cre under the control of the elongation factor-1 alpha (EF1alpha) promoter; includes upstream Kozak modification to increase expression.

   Depositor

   Brian Sauer

   Article

   Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

   Insert

   cre (cre bacteriophage P1)
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-VIVIT

   Plasmid

   #11106

   Depositor

   Anjana Rao

   Article

   Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

   Insert

   VIVIT
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSV Sport RXR alpha

   Plasmid

   #8882

   Depositor

   Bruce Spiegelman

   Article

   Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

   Insert

   RXR alpha (RXRA Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-SHP2 WT

   Plasmid

   #8381

   Depositor

   Ben Neel

   Insert

   SHP2 (PTPN11 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 15, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTD103luxI_sfGFP

   Plasmid

   #48885

   Purpose

   Positive feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

   Insert

   LuxI, sfGFP, and LuxR
   

   Use

   Synthetic Biology

   Tags

   ssrA tag (AANDENYALAA)

   Expression

   Bacterial

   Promoter

   pLux

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG-GFP/cre

    Plasmid

    #49054

    Purpose

    Retroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinase

    Depositor

    Fred Gage

    Article

    Tashiro et al Nat Protoc. 2006;1(6):3049-55.

    Insert

    GFP/Cre
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_nMagHigh1-EGFP-CAAX

    Plasmid

    #67300

    Purpose

    Blue light dependent photoswitching system

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

    Insert

    nMagHigh1-EGFP-CAAX
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs-hSOX2

    Plasmid

    #17218

    Purpose

    Retroviral expression of human SOX2

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223-NTRK2

    Plasmid

    #23883

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    NTRK2 (NTRK2 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pALB-GFP

    Plasmid

    #55759

    Purpose

    Fluorescent marker for hepatocytic lineage

    Depositor

    Snorri Thorgeirsson

    Article

    Heo et al Hepatology. 2006 Dec;44(6):1478-86.

    Insert

    albumin enhancer/promoter (Alb Mouse)
    

    Expression

    Mammalian

    Promoter

    Mouse Albumin Enhancer/Promoter

    Available Since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3-myc3-ROC1

    Plasmid

    #20717

    Depositor

    Yue Xiong

    Article

    He et al Genes Dev. 2006 Nov 1. 20(21):2949-54.

    Insert

    RING finger protein 1 (ROC1) (RBX1 Human)
    

    Tags

    3xMyc

    Expression

    Mammalian

    Available Since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CaMKIIa-hChR2(H134R)-EYFP

    Plasmid

    #26969

    Purpose

    AAV expression of humanized ChR2(H134R) fused to EYFP driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

    Has Service

    AAV1, AAV2, AAV5, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Histidine 134 is mutated to Arginine

    Promoter

    CaMKIIa

    Available Since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. PH-Btk-GFP

    Plasmid

    #51463

    Purpose

    Is a biosensor for PIP3

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    BTK (aa 1–177) (BTK Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW-LIC

    Plasmid

    #26098

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    Three consecutive Taq promoters, 2 active

    Expression

    Bacterial

    Available Since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pLV-dCas9-KRAB-PGK-HygR

    Plasmid

    #83890

    Purpose

    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Promoter

    Human Ubiquitin C Promoter and mouse phosphoglyce…

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. gRNA_GFP-T1

    Plasmid

    #41819

    Purpose

    Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_GFP-T1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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