4,139 results

1. mEmerald-Mito-7

   Plasmid

   #54160

   Purpose

   Localization: Mitochondria, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pdCas9-DNMT3A-PuroR

   Plasmid

   #71667

   Purpose

   Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   CBh

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-AKT-PH-GFP

   Plasmid

   #18836

   Depositor

   Craig Montell

   Article

   Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

   Insert

   AKT-PH domain (AKT1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains amino acids 1-148 (compared to GenBank r…

   Promoter

   CMV

   Available since

   July 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MLM3613

   Plasmid

   #42251

   Purpose

   Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   Streptococcus pyogenes Cas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-Myc-14-3-3ζ

   Plasmid

   #48798

   Purpose

   expresses human myc-14-3-3ζ in mammalian cells

   Depositor

   Huda Zoghbi

   Article

   Chen et al Cell. 2003 May 16;113(4):457-68.

   Insert

   YWHAZ (YWHAZ Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pClneoMyc-LATS2

   Plasmid

   #66852

   Purpose

   Express Myc-tagged LATS2 in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

   Insert

   LATS2 (LATS2 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIRESneo-FLAG/HA Ago2 corrected

   Plasmid

   #10822

   Depositor

   Thomas Tuschl

   Article

   Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

   Insert

   Argonaute 2 (AGO2 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   3 silent mutations in orf

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PL-SIN-Oct4-EGFP

   Plasmid

   #21319

   Purpose

   Lentiviral vector, expresses EGFP under Oct3/4 promoter

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   Enhanced Green Fluorescence Protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Oct-4 promoter region

   Available since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG HRP-TM

   Plasmid

   #44441

   Depositor

   Alice Ting

   Article

   Rhee et al Science. 2013 Jan 31.

   Insert

   Horseradish peroxidase fused to the transmembrane domain of PDGFR
   

   Use

   Tags

   HA tag and myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ub-M-GFP

    Plasmid

    #11938

    Depositor

    Nico Dantuma

    Article

    Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

    Insert

    Ub-M-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of GFP. Methionine …

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MSCV-N-Flag-HA-IRES-PURO

    Plasmid

    #41033

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Type

    Empty backbone

    Use

    Retroviral; Gateway destination vector

    Tags

    Flag, HA, and IRES Puro

    Expression

    Mammalian

    Mutation

    Promoter

    LTR-driven expression

    Available since

    Oct. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA4-Chk1-Flag

    Plasmid

    #22894

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

    Insert

    Chk1 (CHEK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRK5-myc-Rac1-Q61L

    Plasmid

    #12983

    Depositor

    Gary Bokoch

    Insert

    Rac1 constitutively active (RAC1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3 HA human NEMO

    Plasmid

    #13512

    Depositor

    Kunliang Guan

    Article

    Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

    Insert

    NEMO (IKBKG Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EBFP2-C1

    Plasmid

    #54665

    Purpose

    Localization: C1 Cloning Vector, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. p3xFLAG-CMV10-hFIP200

    Plasmid

    #24300

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-UtrCH

    Plasmid

    #26737

    Depositor

    William Bement

    Article

    Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

    Insert

    Utrophin
    

    Use

    Reporter for f-actin

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Calponin homology domain of utrophin (aa# 1-261).…

    Promoter

    CMV IE94

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pk7-GR-GFP

    Plasmid

    #15534

    Depositor

    Ian Macara

    Article

    Carey et al J Cell Biol. 1996 Jun . 133(5):985-96.

    Insert

    Glucocorticoid receptor (NR3C1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The glucocorticoid receptor was cloned into the X…

    Promoter

    Available since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET259-pUC57 24xMS2V6

    Plasmid

    #104391

    Purpose

    MS2V6 cloning

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. phage-cmv-cfp-24xms2

    Plasmid

    #40651

    Depositor

    Robert Singer

    Article

    Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

    Insert

    CFP-24xMBS
    

    Use

    Lentiviral

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge