Addgene

We narrowed to 26,338 results for: CAL

Showing: 5801 - 5840 of 26338 results

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pFRETgc-2in1-CN

Plasmid

#105118

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mCherry and mEGFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-EYFP-WPRE

Plasmid

#104104

Purpose

Expresses EYFP under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDUET-ctCPR-A17AMO

Plasmid

#20115

Depositor

Jay Keasling

Article

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

p450 reductase and amorphadiene oxidase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
UAS-Halo7::CAAX

Plasmid

#87645

Purpose

For creating drosophila transgenics expressing UAS-Halo7::CAAX

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Halo7::CAAX

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBad-UXPS

Plasmid

#172138

Purpose

bacteria expression plasmid for a UDP/UTP sensor

Depositor

Huiwang Ai

Article

Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

Insert

UXPS

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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SIRT5H158Y-pET15b

Plasmid

#81032

Purpose

Bacterial expression of Human catalytic mutant SIRT5 protein

Depositor

David Lombard

Article

Kumar et al Methods Mol Biol. 2016;1436:241-57. doi:

Insert

Sirt5 (SIRT5 Human)

Use

Tags

His

Expression

Bacterial

Mutation

H158Y catalytic mutant

Promoter

T7

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-mE6-AP/2591a

Plasmid

#11468

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

mE6-AP (Ube3a Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

n/a

Promoter

Available since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
pFRETgc-2in1-NN

Plasmid

#105124

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mEGFP/mCherry

Expression

Plant

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Integrin-αv Ectodomain M400C

Plasmid

#159642

Purpose

Contains integrin alpha v residues 1–960 with the M400C mutation

Depositor

Timothy Springer

Article

Wang et al Proc Natl Acad Sci U S A. 2017 May 23;11

Insert

Integrin alpha v ectodomain

Use

Tags

Expression

Mammalian

Mutation

M400C

Promoter

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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HS-mKate2

Plasmid

#105945

Purpose

heat shock inducible transgenesis, cytosolic mKate2

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

mKate2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET11a-STEPtag

Plasmid

#159373

Purpose

Expresses STEPtag in E. coli

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

STEPtag

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTGFP-T7-Hha10T

Plasmid

#65427

Purpose

Parent vector for constructing non-replicating lesion-bearing plasmid

Depositor

Yinsheng Wang

Article

You et al Nat Protoc. 2015 Sep;10(9):1389-406. doi

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP Q157TAG

Plasmid

#68297

Purpose

Q157TAG GFP reporter

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

GFP Q157TAG

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-TRE-HaloTag-WPRE

Plasmid

#104108

Purpose

Expresses HaloTag under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

HaloTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-P-RAN-Gelsolin

Plasmid

#106413

Purpose

P-RAN-Gelsolin (RANbody Gelsolin::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-Gelsolin

Use

Tags

HA and His

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGST2-ALIX (795-811)

Plasmid

#21502

Depositor

James Hurley

Article

Lee et al Science. 2008 Oct 24. 322(5901):576-80.

Insert

ALIX (PDCD6IP Human)

Use

Tags

GST

Expression

Bacterial

Mutation

none

Promoter

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
His-tagged-SF1

Plasmid

#28182

Depositor

Bernard Schimmer

Article

Frigeri et al Mol Endocrinol. 2000 Apr . 14(4):535-44.

Insert

SF1 (Nr5a1 Mouse)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pBAD-hsGFP

Plasmid

#64911

Purpose

Encodes a Fluorescent Reporter for Hydrogen Sulfide in E. Coli

Depositor

Huiwang Ai

Article

Chen et al Biochemistry. 2014 Sep 23;53(37):5966-74

Insert

hsGFP

Use

Tags

His Tag

Expression

Bacterial

Mutation

Chromophore tyrosine of the protein mutated to TA…

Promoter

araBAD

Available since

May 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-TO-PV-NLS-Dsred

Plasmid

#16341

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

PV-DsRed-NLS (Pvalb Rat)

Use

Tags

Myc and NLS

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pACEBac1-GCP5

Plasmid

#178071

Purpose

Insect cell expression of GCP5

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

GCP5 (TUBGCP5 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAH-Golgi-bapaUGAc

Plasmid

#201675

Purpose

boronic acid- and peptide-assisted UDP-GlcNAc sensor in Golgi

Depositor

Huiwang Ai

Article

Zhang et al ACS Sens. 2023 Jul 22. doi: 10.1021/acss

Insert

Golgi-bapaUGAc

Use

Tags

Golgi leading sequence

Expression

Mammalian

Mutation

Promoter

CMV SP6

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2-elavl3_NES-rsCaMPARI-mRuby3

Plasmid

#122129

Purpose

Pan-neuronal expression of rsCaMPARI in zebrafish, nucleus excluded

Depositor

Eric Schreiter

Article

Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

Insert

rsCaMPARI

Use

Zebrafish expression

Tags

NES-His and mRuby3

Expression

Mutation

Promoter

elavl3

Available since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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UAS-7xHalo7::CAAX

Plasmid

#87647

Purpose

For creating drosophila transgenics expressing UAS-7xHalo7::CAAX

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

7xHalo7::CAAX

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pVHed05

Plasmid

#82351

Purpose

Contains PBAD-TP901 and Ptet-Bxb1 as well as constitutive TetR

Depositor

Richard Murray

Article

Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do

Inserts

TP901-1 integrase
Bxb1 integrase
AraC (araC )
TetR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-T3-hCASeGFP-pA

Plasmid

#59766

Purpose

Expresses CAS9-eGFP fusion protein in mammalian cells.

Depositor

Wataru Fujii

Article

Fujii et al Nucleic Acids Res. 2013 Aug 30.

Insert

CAS9

Use

Tags

Flag and eGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Bongwoori-R3

Plasmid

#101658

Purpose

A variant of Bongwoori with H237R mutation in its linker region. (* super ecliptic pHluorin A227D)

Depositor

Bradley Baker

Article

Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

Insert

Bongwoori-R3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-TRE-SNAPf-WPRE

Plasmid

#104106

Purpose

Expresses SNAPf under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

SNAPf

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-H-RAN-GFP1

Plasmid

#106418

Purpose

H-RAN-GFP1 (RANbody GFP1::Sm-HAtag)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

H-RAN-GFP1

Use

Tags

HA, His, and Multiple HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Connexin43-GFP-APEX

Plasmid

#44440

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

Connexin43 -EGFP - pea APEX

Use

Tags

Expression

Mammalian

Mutation

K14D, W41F, E112K mutations on APEX

Promoter

CMV

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
HT-mGold

Plasmid

#183986

Purpose

Expresses fluorescent HaloTag7 fusion protein in mammalian cells in the whole cell.

Depositor

Pablo Rivera-Fuentes

Article

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

HaloTag-mGold

Use

Tags

HaloTag, mGold

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Rab9

Plasmid

#31736

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab9 (Rab9 Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Actin

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
MAC_C_DUX4

Plasmid

#187799

Purpose

MAC-tagged gene expression of human DUX4

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

DUX4 (DUX4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-P-RAN-RFP4

Plasmid

#106410

Purpose

P-RAN-RFP4 (RANbody RFP4::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-RFP4

Use

Tags

HA and His

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL005

Plasmid

#92327

Purpose

Express FtsZ tagged with mEos3.2 in E coli

Depositor

Jie Xiao

Article

Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

Insert

FtsZ

Use

Tags

mEos3.2

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRETvr-2in1-CC

Plasmid

#105117

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mVenus/tagRFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFF145

Plasmid

#61446

Purpose

aTc-inducible Beta recombinase

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

Beta recombinase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pTetO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL0_13 [pIP501 ORI]

Plasmid

#198927

Purpose

Level 0 part

Depositor

Cultivarium Tools, Nili Ostrov, Charlie Gilbert

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIP501 ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKS13

Plasmid

#24627

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

pdc, adhB

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
pSS2 (hS100B)

Plasmid

#26774

Depositor

Gary Shaw

Article

Smith et al Biochemistry. 1996 Jul 9. 35(27):8805-14

Insert

S100B (S100B Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
OA-961B

Plasmid

#104967

Purpose

D. suzukii Medea Construct

Depositor

Omar Akbari

Article

Buchman et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

Inserts

GFP
dsRed
HR5Ie1

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 5801 - 5840 of 26338 results

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