We narrowed to 7,762 results for: loxP

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pEJS1005-Lenti-mammalian c.o.AcrIIA5-FLAG-NLS-HygR

Plasmid

#136514

Purpose

Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells in a lentiviral vector

Depositor

Erik Sontheimer

Publication

Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

Insert

Codon-optimized AcrIIA5

Use

Lentiviral

Tags

FLAG/NLS

Expression

Mammalian

Promoter

SFFV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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p21-WT-MEFV

Plasmid

#134702

Purpose

Lentiviral expression of human MEFV after doxycycline administration

Depositor

Thomas Henry

Publication

Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Mutation

WT

Promoter

TRE2 (Dox-inducible)

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCRISPaint-T2A-Gal4DBD-3xP3-RFP

Plasmid

#127564

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Publication

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Gal4DBD

Use

CRISPR

Expression

Insect

Available since

July 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLPCX-Cx43-FS-IRES-GFP

Plasmid

#65438

Purpose

Mammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral production

Depositor

Trond Aasen

Publication

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

Cx43 (GJA1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

frameshift (FS) mutation 35delA

Promoter

CMV

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA1_IL1RN

Plasmid

#64140

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pJZC77

Plasmid

#62338

Purpose

sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA
COM-KRAB

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…

Promoter

CMV and U6

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEMS1062

Plasmid

#29011

Purpose

The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

Depositor

Elizabeth Simpson

Publication

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple155-Cre/EGFP-NLS

Use

High copy number homologous recombination plasmid…

Expression

Mammalian

Promoter

MiniPromoter Ple155 from the human PCP2 gene

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)

Plasmid

#215010

Purpose

Express a Mitophagy reporter in mammalian cells (untested)

Depositor

Wade Harper

Insert

pSu9 (Presequence of Fo-ATPase subunit 9)

Use

Lentiviral

Tags

HaloTag(7)-mEGFP

Expression

Mammalian

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-TAZ

Plasmid

#213587

Purpose

Doxycycline inducible expression of TAZ wild type cDNA in mammalian cells

Depositor

Channing Der

Publication

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

TAZ (WWTR1 Human)

Use

Lentiviral

Tags

2xHA

Expression

Mammalian

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-C-Myc-DDK-IRES-Puro METTL3

Plasmid

#200724

Purpose

Lentiviral expression of Flag-Tagged human METTL3

Depositor

Claudio Alarcón

Insert

METTL3 (METTL3 Human)

Use

Lentiviral

Tags

Myc-DDK from backbone plasmid

Expression

Mammalian

Promoter

CMV

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDB-hPER2-Luciferase-hvTK-bla

Plasmid

#179450

Purpose

Donor vector to knock in firefly Luciferase C-terminal to human PER2 gene

Depositor

Achim Kramer

Publication

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Luciferase

Use

CRISPR

Tags

His/Flag

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDB-hCRY1-mScarlet-I-CD4-bla

Plasmid

#179442

Purpose

Donor vector to knock in mScarlet-I C-terminal to human CRY1 gene

Depositor

Achim Kramer

Publication

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarletI

Use

CRISPR

Tags

His/Flag

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-SynI-CreOn-FlpOff-mNaChBacMUT-P2A-EGFP

Plasmid

#176281

Purpose

Viral vector for co-expression of non-functional NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Publication

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

mNaChBacMUT-P2A-EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

E191K

Promoter

human Synapsin I

Available since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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3xFlag-HaloTag-EZH2 HDR - pDY053

Plasmid

#171108

Purpose

Homology directed repair plasmid for generating HaloTag-EZH2 cells

Depositor

Thomas Cech

Publication

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EZH2 (EZH2 Human)

Tags

HaloTag

Expression

Mammalian

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWPXL-p150

Plasmid

#160992

Purpose

Express fulllength of mouse p150 protein

Depositor

Zhiguo Zhang

Publication

Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CHAF1A (Chaf1a Mouse)

Tags

GFP

Expression

Mammalian

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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HES7-NLuc-2A-tdTomato

Plasmid

#130932

Purpose

Donor vector for tagging human HES7 C-terminus with NanoLuc and tdTomato

Depositor

James Thomson

Publication

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HES7 (HES7 Human)

Tags

NanoLuc and tdTomato

Expression

Mammalian

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pS6-hα2M-H6

Plasmid

#128495

Purpose

Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Publication

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

A2M (G27-S1468) (A2M Human)

Tags

His tag (6x)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCLXEBR-pTF-Ngn3

Plasmid

#114307

Purpose

TET-inducible expression of the Neugenin 3 transcription factor

Depositor

Patrick Salmon

Publication

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Insert

Ngn3 (NEUROG3 Human)

Use

Lentiviral

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Flag3-pG-mNanog-pgk-hph

Plasmid

#74911

Purpose

Mammalian expression of Flag tagged mNanog

Depositor

Jose Silva

Publication

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nanog (Nanog Mouse)

Tags

3xFlag

Expression

Mammalian

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX(cre)-OCaMP-WPRE

Plasmid

#229853

Purpose

pAAV vector for Cre-dependent OCaMP (orange calcium indicator) expression under the control of hSyn promoter

Depositor

Alexander Lohman

Publication

Aggarwal et al Nat Commun. 2026 Jun 22. doi: 10.1038/s4

Insert

OCaMP

Use

AAV

Tags

6xHis

Expression

Mammalian

Available since

Aug. 25, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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