We narrowed to 7,345 results for: Lif;

1. pUFlip-floxed2A-KalTA4-; gcry1:BFP -2

   Plasmid

   #173891

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-KalTA4; gcry1: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-uTEV1-MAVS-mTA6

   Plasmid

   #171004

   Purpose

   HiLITR protease with MAVS [Y536delinsFIVLI, R537A, R538A] C-terminal tmd targeting information (Mitochondria)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]

   Promoter

   pTRE-tight (TetOn)

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSMT3-FBF2 SS/Y

   Plasmid

   #166973

   Purpose

   Expresses FBF-2 with SS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

   Depositor

   Traci Hall

   Article

   Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

   Insert

   FBF-2 (fbf-2 Nematode)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   FBF-2 PUM RNA binding domain; amino acids 164-575…

   Promoter

   T7

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSMT3-FBF2 AS/Y

   Plasmid

   #166974

   Purpose

   Expresses FBF-2 with AS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

   Depositor

   Traci Hall

   Article

   Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

   Insert

   FBF-2 (fbf-2 Nematode)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   FBF-2 PUM RNA binding domain; amino acids 164-575…

   Promoter

   T7

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)

   Plasmid

   #91806

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Residues A1529-G1534 replaced with a PreScission …

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)

   Plasmid

   #91807

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   PreScission Protease site (LEVLFQGP) inserted bet…

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

   Plasmid

   #91818

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed lysine 1355 and lysine 1435 to alanines; …

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP N1 AURKA deltaNter

   Plasmid

   #157753

   Purpose

   Expression of AURKA without Nter, fused to GFP in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p1A Prk-

   Plasmid

   #162694

   Purpose

   p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   H. neapolitanus CbbLS and S. elongatus Prk
   

   Use

   Tags

   N terminal 6x His tag on PRK

   Expression

   Bacterial

   Mutation

   Prk inactive (K20M S21A native protein, this map …

   Promoter

   PLtet0-1 promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)

    Plasmid

    #123340

    Purpose

    Negative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-EKAR-EV(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Venus-cpVenus-FLARE-CKAR(T/A)

    Plasmid

    #123344

    Purpose

    Negative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-CKAR(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR

    Plasmid

    #123336

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-cpCerulean3-FLARE-AKAR
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Anti-Mad3 [N129A/6R]

    Plasmid

    #140075

    Purpose

    Mammalian Expression Plasmid of anti-Mad3 (Human). Derived from hybridoma N129A/6.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Mad3 (Human) recombinant mouse monoclonal antibody (MXD3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-GABARα6N-HA

    Plasmid

    #128111

    Purpose

    Express HA tagged GABARα6 N terminal domain

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Gabra6 (Gabra6 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Anti-Synaptotagmin-12 [N277/7R]

    Plasmid

    #114482

    Purpose

    Mammalian Expression Plasmid of anti-Synaptotagmin-12 (Mouse). Derived from hybridoma N277/7.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Synaptotagmin-12 (Mus musculus) recombinant mouse monoclonal antibody (Syt12 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJET1.2_Bod1l_Sense

    Plasmid

    #124443

    Purpose

    Plasmid for sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    Biorientation of chromosomes in cell division 1-like (Bod1l Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-Hemmar-DSCP-ftz-FLP(D)

    Plasmid

    #119894

    Purpose

    FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intron

    Depositor

    Rita Sousa-Nunes

    Article

    Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

    Inserts

    FLP(D)
    DSCP-ftz (ftz Fly)
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    a mutated form of FLP, which at position 5 contai…

    Promoter

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMAL c2x Tral N

    Plasmid

    #113009

    Purpose

    For bacterial expression of MBP fusion of N terminal region of Drosophila Tral

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    N terminus of tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amino acids 1-355

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMAL c2x Tral FL-15A

    Plasmid

    #113011

    Purpose

    For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    15 PNG phosphorylation sites mutated to A

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMAL c2x Tral C

    Plasmid

    #113008

    Purpose

    For bacterial expression of MBP fusion of C terminal region of Drosophila Tral

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    C terminus of tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amino acids 356-652

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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