-
Plasmid#162155PurposeExpresses FastLightR-bRaf-mVenus in mammalian cellsDepositorInsertFastLightR-bRaf-mVenus
UseTagsmVenusExpressionMammalianMutationValine 600 is substituted with Glutamic acidPromoterCMVAvailable sinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
ORCO-T2A-QF-DBD
Plasmid#162525PurposePlasmid for CRISPR-mediated knock-in line that expresses the DNA-binding domain of QF by knocking-in a T2A-QF-DBD fragment replacing the stop codon of the endogenous Aedes aegypti Orco geneDepositorInsertOrco-left-HDR-arm; T2A-Zip-::QFDBD-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
eDrcKIT
Plasmid#184001PurposeOptically controlled eDrcKIT expression in mammalian cellsDepositorInserteDrcKIT (KIT Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T240A
Plasmid#111457PurposeTransient expression of Flag-tagged human Sox9 with T240A mutationDepositorInsertSox9 (SOX9 Human)
UseTagsFlagExpressionMammalianMutationT240APromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Golgi-7
Plasmid#57959PurposeLocalization: Gal-T - Golgi Complex, Excitation: 549, Emission: 565DepositorInsertGolgi (B4GALT1 Human)
UseTagsmOrange2ExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
ppk301-T2A-QF2 HDR plasmid
Plasmid#130667PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 geneDepositorInsertppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
UseDonor templateTagsExpressionMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23_Promoter_1-3
Plasmid#210629PurposeLuciferase experiment for TAL1 promoter 1-3DepositorInsertpromoter 1-3 (TAL1 Human)
UseTagsExpressionMammalianMutationPromoterMinimal PromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a_ASCL5_P2A_Hygro_Barcode
Plasmid#120430PurposeBarcoded lentiviral vector to express ASCL5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertASCL5 (ASCL5 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shMKP5.v2 puro
Plasmid#87793PurposeExpresses an inducible short hairpin targeting human MKP5 sequenceDepositorInsertshMKP5 (DUSP10 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE tightAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shMKP5.v1 puro
Plasmid#87792PurposeExpresses an inducible short hairpin targeting human MKP5 sequenceDepositorInsertshMKP5 (DUSP10 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE tightAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-siResist
Plasmid#49852PurposeEncodes human connexin 43 with wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
UseTagsExpressionMammalianMutationContains several wobble mutations to confer resis…PromoterCMVAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
LightR-bRaf-mVenus
Plasmid#162154PurposeExpresses LightR-bRaf-mVenus in mammalian cellsDepositorInsertLightR-bRaf-mVenus
UseTagsmVenusExpressionMammalianMutationValine 600 is substituted with Glutamic acidPromoterCMVAvailable sinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T236A/T240A
Plasmid#111458PurposeTransient expression of Flag-tagged human Sox9 with T236A/T240A mutationDepositorInsertSox9 (SOX9 Human)
UseTagsFlagExpressionMammalianMutationT236A and T240APromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP1
Plasmid#129504PurposeExpresses AvicFP1 constitutively in E. coli (most strains)DepositorInsertAvicFP1
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausGFP
Plasmid#129503PurposeExpresses AausGFP constitutively in E. coli (most strains)DepositorInsertAausGFP
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJM5-mDPYD
Plasmid#61614PurposeThe mouse Dpyd was used to rescue the human DPYD hairpin affect on EMTDepositorInsertdihydropyrimidine dehydrogenase (Dpyd Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP2
Plasmid#129505PurposeExpresses AvicFP2 constitutively in E. coli (most strains)DepositorInsertAvicFP2
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK-caCas9-NatMX-Neut5L-Ade2 drive
Plasmid#89577PurposecaCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locusDepositorInsertAde2 gene drive
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP2
Plasmid#129500PurposeExpresses AausFP2 constitutively in E. coli (most strains)DepositorInsertAausFP2
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Hygro-sgSIK3
Plasmid#138699PurposeExpresses a human SIK3-targeting sgRNA and Cas9DepositorInsertsgSIK3 human (SIK3 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only