We narrowed to 6,040 results for: WUS;

1. NmeCas9_3XNLS_pMCSG7

   Plasmid

   #120078

   Purpose

   Bacterial expression of wtNmeCas9 with 3X NLS

   Depositor

   Erik Sontheimer

   Article

   Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

   Insert

   NmeCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PL452-hGH-polyA-PGK-neoR

   Plasmid

   #191681

   Purpose

   Donor plasmid backbone PGK-neoR

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. LV_EF1a_CDK1-P2A-Hygro_Barcode

   Plasmid

   #170221

   Purpose

   Barcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CDK1 (CDK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-kanMX6-P41nmt1-GFP

   Plasmid

   #39290

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

   Promoter

   Available since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pMV-LEU2

   Plasmid

   #65334

   Purpose

   standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUs

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   LEU2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV E2F1 1-284

   Plasmid

   #24216

   Purpose

   Mammalian expression of human E2F1 (aa 1-284) with HA tag

   Depositor

   Kristian Helin

   Article

   Helin et al Genes Dev. 1993 Oct . 7(10):1850-61.

   Insert

   E2F1 1-284 (E2F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

7. p53-(1-393)-R175H-N268D

   Plasmid

   #35086

   Depositor

   Cheryl Arrowsmith

   Article

   Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains aa 1-393 - R175H-N268D

   Promoter

   T7

   Available since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pC1300_pUB10_pco-vCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

   Plasmid

   #197966

   Purpose

   T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pco-vCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CmYLCVp and pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LV_EF1a_CCNB1-P2A-Hygro_Barcode

   Plasmid

   #170218

   Purpose

   Barcoded lentiviral vector to express CCNB1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CCNB1 (CCNB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. MTK3_009

    Plasmid

    #123724

    Purpose

    Encodes nuclear localized PHYB fused to activating domain of VP16 as Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PHYB-VP16-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIG-Flag-Sox9-IRES-nls-GFP

    Plasmid

    #44281

    Depositor

    Martin Cheung

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

    Insert

    Flag-Sox9 (SOX9 Chicken)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE enhancer and Chicken beta-actin promoter

    Available since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

12. Lenti-SLC35B2-sgRNA

    Plasmid

    #154860

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 sgRNA (SLC35B2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. EF1a_ASCL3_P2A_Hygro_Barcode

    Plasmid

    #120428

    Purpose

    Barcoded lentiviral vector to express ASCL3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    ASCL3 (ASCL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTK0_015

    Plasmid

    #123930

    Purpose

    Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB Destination - Insulator + tagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. MTK0_017

    Plasmid

    #123932

    Purpose

    Encodes the BxBI attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    BxBI attB tagBFP destination vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. TIAM1_PDZ_1

    Plasmid

    #103925

    Purpose

    Protein expression and purification of TIAM PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTorPE-GEM-GECO1

    Plasmid

    #32463

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEM-GECO1
    

    Use

    Lab constructed

    Tags

    6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…

    Expression

    Bacterial

    Mutation

    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-FLAG-BMAL1-S513A

    Plasmid

    #186831

    Purpose

    Expresses FLAG-BMAL1-S513A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 513 to Alanine

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-FLAG-BMAL1-S515A

    Plasmid

    #186832

    Purpose

    Expresses FLAG-BMAL1-S515A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 515 to Alanine

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-FLAG-BMAL1-S516A

    Plasmid

    #186833

    Purpose

    Expresses FLAG-BMAL1-S516A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 516 to Alanine

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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