We narrowed to 6,263 results for: wus

1. pDA007

   Plasmid

   #131380

   Purpose

   ORFeus expression, WT

   Depositor

   Kathleen Burns

   Article

   Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

   Insert

   ORFeus-Hs
   

   Use

   Tags

   ORF2-3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PCS2-FlipGFP(Casp3 cleavage seq)

   Plasmid

   #124432

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq)
   

   Use

   Zebrafish (good expression)

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. L4440_BioBrick-hif-1-sgRNA-B

   Plasmid

   #177785

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hif-1 (hif-1 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PL452-hGH-polyA-PGK-neoR

   Plasmid

   #191681

   Purpose

   Donor plasmid backbone PGK-neoR

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDA025

   Plasmid

   #131384

   Purpose

   ORFeus expression, mutant

   Depositor

   Kathleen Burns

   Article

   Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

   Insert

   ORFeus
   

   Use

   Tags

   ORF2-3xFlag

   Expression

   Mammalian

   Mutation

   H230A En

   Promoter

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDA034

   Plasmid

   #131386

   Purpose

   ORFeus expression, mutant

   Depositor

   Kathleen Burns

   Article

   Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

   Insert

   ORFeus
   

   Use

   Tags

   ORF2-3xFlag

   Expression

   Mammalian

   Mutation

   D702Y RT

   Promoter

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

   Plasmid

   #42337

   Purpose

   Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. MiniCoopR U6:gRNA spred1, mitfa:Cas9

   Plasmid

   #118843

   Purpose

   Targets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   spred1 gRNA (spred1 Zebrafish)
   

   Use

   CRISPR; Tol2

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-PSPC1-CT

   Plasmid

   #124374

   Purpose

   expresses EGFP-PSPC1-CT131 in mammalian cell

   Depositor

   Yuh-Shan Jou

   Article

   Lang et al Nat Commun. 2019 Dec 16;10(1):5716. doi:

   Insert

   PSPC1 CT (PSPC1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PCDNA3-GFP1-9 T2A mCherry

    Plasmid

    #124430

    Purpose

    Expresses GFP1-9 and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    GFP1-9 T2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDA027

    Plasmid

    #131385

    Purpose

    ORFeus expression, mutant

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    ORFeus
    

    Use

    Tags

    ORF2-3xFlag

    Expression

    Mammalian

    Mutation

    H230A, D702Y

    Promoter

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 human cofilin S3E

    Plasmid

    #50855

    Purpose

    expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 S3E (CFL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S3E psuedo-phosporylated, non-activatable

    Promoter

    CMV

    Available Since

    Feb. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

    Plasmid

    #124493

    Purpose

    Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipYFP(TEV cleavage seq) T2A mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode

    Plasmid

    #170248

    Purpose

    Barcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    C124S

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-UMI

    Plasmid

    #222694

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Promoter

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET41-MBP-t-A3A-t-His

    Plasmid

    #109231

    Purpose

    Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease

    Depositor

    Rahul Kohli

    Article

    Schutsky et al Nat Biotechnol. 2018 Oct 8. pii: nbt.420

    Insert

    human APOBEC3A (APOBEC3A Human, Optimized for E. coli expression)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EF1a_KLF7_P2A_Hygro_Barcode

    Plasmid

    #120493

    Purpose

    Barcoded lentiviral vector to express KLF7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF7 (KLF7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3 Flag Jnk1a1

    Plasmid

    #13798

    Depositor

    Roger Davis

    Article

    Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

    Insert

    Jnk1a1 (MAPK8 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EF1a_MYC_P2A_Hygro_Barcode

    Plasmid

    #120461

    Purpose

    Barcoded lentiviral vector to express MYC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LV_EF1a_CCNB1-P2A-Hygro_Barcode

    Plasmid

    #170218

    Purpose

    Barcoded lentiviral vector to express CCNB1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CCNB1 (CCNB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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