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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,678 results for: nin

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Showing: 2941 - 2960 of 66678 results

  3. bZiPro

    Plasmid
    #86846
    Purpose
    express NS2B-NS3 protease in E.coli
    Depositor
    Dahai Luo
    Publication
    Zhang et al Science. 2016 Dec 8. pii: aai9309.
    Inserts
    NS3 1-177
    NS2B45-96
    Tags
    a hexahistidine tag and a thrombin cleavage site
    Expression
    Bacterial
    Mutation
    NS2B cofactor region (residues 45–96) and NS3 pro…
    Promoter
    T7
    Available since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86846-plasmid-map-sequence-id-172845

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  7. pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3

    Plasmid
    #127354
    Purpose
    gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Publication
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TurboID (BirA mutant)
    Use
    Gateway Cloning
    Tags
    GS linker, V5, and mVenus
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127354-plasmid-map-sequence-id-246338

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  15. pcDNA C12 ecDHFR DD YFP HA

    Plasmid
    #192815
    Purpose
    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.
    Depositor
    John Hulleman
    Publication
    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    E. coli dihydrofolate reductase
    Tags
    enhanced yellow fluorescent protein and hemagglut…
    Expression
    Mammalian
    Mutation
    W74R/T113S/E120D/Q146L
    Promoter
    CMV
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192815-plasmid-map-sequence-id-377240

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  17. pCW57.1 Lysosomal-METRIQ

    Plasmid
    #135401
    Purpose
    Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity
    Depositor
    Eisuke Itakura
    Publication
    Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10
    Insert
    DNAse II alpha (DNASE2 Human)
    Use
    Lentiviral
    Tags
    T2A, mCherry, and sfGFP
    Expression
    Mammalian
    Mutation
    Single-nucleotide polymorphism C432T
    Available since
    Feb. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135401-plasmid-map-sequence-id-273129

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Showing: 2941 - 2960 of 66678 results