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We narrowed to 9,048 results for: sgRNA

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Showing: 2941 - 2960 of 9048 results

  5. pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139096
    Purpose
    Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dxCas9 3.7
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139096-plasmid-map-sequence-id-273894

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  8. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available Since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  14. pGGI_RT_Sa_sgRNA

    Plasmid
    #136982
    Purpose
    Goldengate/Greengate module for genome editing, using the Sa guideRNA.
    Depositor
    Klaus Theres
    Insert
    Sa guide RNA
    Use
    CRISPR
    Available Since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    136982-plasmid-map-sequence-id-270593

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  15. pGGD_RT_Sa_sgRNA

    Plasmid
    #136975
    Purpose
    Goldengate/Greengate module for genome editing, using the Sa guideRNA.
    Depositor
    Klaus Theres
    Insert
    Sa guide RNA
    Use
    CRISPR
    Available Since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    136975-plasmid-map-sequence-id-270568

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  16. pGGE_RT_Sa_sgRNA

    Plasmid
    #136978
    Purpose
    Goldengate/Greengate module for genome editing, using the Sa guideRNA.
    Depositor
    Klaus Theres
    Insert
    Sa guide RNA
    Use
    CRISPR
    Available Since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136978-plasmid-map-sequence-id-269094

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Showing: 2941 - 2960 of 9048 results