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pCS2_IRES2_EGFP_KCNQ2 var4

Plasmid

#173154

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Promoter

Simian CMV IE94

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pHR CD19-mMegf10 CAR

Plasmid

#113013

Purpose

for production of lentivirus encoding the CD19 CAR-Pmegf10

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD19 CAR-P Megf10 intracellular domain (CD19, Megf10 Mouse, Human, Synthetic)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TCF21

Plasmid

#192894

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of TCF21

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

TCF21 (TCF21 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTMiniTrex-mCherry-Shark3

Plasmid

#233145

Purpose

Serves as a template plasmid for amplifying Shark3 (Theo-OFF) hammerhead aptazyme-3xTy-tagging cassette

Depositor

Ronald Etheridge

Article

Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

Inserts

mCherry
Shark3

Use

Prokaryotic expression

Tags

3x Ty tag

Mutation

Codon optimized for Trypanosoma cruzi and changed…

Available Since

March 20, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pTMiniTrex-mCherry-Shark1

Plasmid

#233143

Purpose

Serves as a template plasmid for amplifying Shark1 (Tet-OFF) hammerhead aptazyme-3xTy-tagging cassette

Depositor

Ronald Etheridge

Article

Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

Inserts

mCherry
Shark1

Use

Prokaryotic expression

Tags

3x Ty tag

Mutation

Codon optimized for Trypanosoma cruzi and changed…

Available Since

March 25, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pUFlip-floxed2A-mRFP-; gcry1:BFP -2

Plasmid

#173888

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEv-Fat4-citrine

Plasmid

#112854

Purpose

Constitutive expression of human fat4 protein fused to citrine fluorescent protein

Depositor

David Sprinzak

Article

Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 1

Insert

FAT4 (FAT4 Human)

Tags

Citrine

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pHR EGFR-CD3ε-FusionRed

Plasmid

#188626

Purpose

Human EGFR labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al Elife. 2023 May 22;12:e82863. doi: 10.75

Insert

EGFR (EGFR Human, Synthetic)

Use

Lentiviral

Tags

CD3ε-FusionRed

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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ACE2

Plasmid

#164219

Purpose

Expresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection marker

Depositor

Sriram Neelamegham

Article

Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

Insert

ACE2 (ACE2 Human)

Use

Lentiviral

Mutation

silent mutation to remove internal EcoRI site (GA…

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-LTK-V5/HIS

Plasmid

#200116

Purpose

Expression of human LTK receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human LTK receptor tyrosine kinase (LTK Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-GCaMP7f-CCR5TC

Plasmid

#216534

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to GCaMP7f, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

GCaMP7f

Expression

Mammalian

Promoter

CAG

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-NR1H2

Plasmid

#192919

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of NR1H2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

NR1H2 (NR1H2 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Pannexin-2 [N121A/31R]

Plasmid

#140073

Purpose

Mammalian Expression Plasmid of anti-Pannexin-2 (Rat). Derived from hybridoma N121A/31.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pannexin-2 (Rat) recombinant mouse monoclonal antibody (Panx2 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-RNF51

Plasmid

#126546

Purpose

Expresses GFP-tagged RNF51 in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF51 (BMI1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

LTR

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

Plasmid

#87828

Purpose

High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.

Depositor

Andrea Musacchio

Article

Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

Inserts

NLS
PTS1

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV promoter, tetracycline operator

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; gcry1:BFP -0

Plasmid

#173886

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-H2BmGreenLantern

Plasmid

#177332

Purpose

To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

Depositor

Murray Blackmore, Gregory Petsko, Pantelis Tsoulfas

Article

Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

Insert

H2B (Hist1h2bq Rat)

Use

AAV

Tags

mGreenLantern

Expression

Mammalian

Mutation

This fusion protein was optimized to the Human co…

Promoter

CAG

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT-B-GRPA

Plasmid

#226222

Purpose

Human RPA34 tagged to GFP under a EF1alpha promotor for generating stable HeLa Kyoto GFP-RPA34 using the Flp-In recombination system

Depositor

Cristina Cardoso

Article

Pabba et al Elife. 2023 Oct 31;12:RP87572. doi: 10.7

Insert

RPA34 (RPA2 Human)

Expression

Mammalian

Promoter

EF1ALPHA

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ALK-V5/HIS

Plasmid

#200115

Purpose

Expression of human ALK receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human ALK receptor tyrosine kinase (ALK Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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NLS-PyronicSF/pCMV-myc-nuc

Plasmid

#124814

Purpose

Highly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.

Depositor

Luis Felipe Barros

Article

Arce-Molina et al Elife. 2020 Mar 6;9. pii: 53917. doi: 10

Insert

PyronicSF

Tags

Nuclear Localization Signal

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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