We narrowed to 7,519 results for: Lif

1. pEGFP N1 AURKA deltaNter

   Plasmid

   #157753

   Purpose

   Expression of AURKA without Nter, fused to GFP in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p1A Prk-

   Plasmid

   #162694

   Purpose

   p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   H. neapolitanus CbbLS and S. elongatus Prk
   

   Use

   Tags

   N terminal 6x His tag on PRK

   Expression

   Bacterial

   Mutation

   Prk inactive (K20M S21A native protein, this map …

   Promoter

   PLtet0-1 promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)

   Plasmid

   #123340

   Purpose

   Negative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-EKAR-EV(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Venus-cpVenus-FLARE-CKAR(T/A)

   Plasmid

   #123344

   Purpose

   Negative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-CKAR(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR

   Plasmid

   #123336

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Cerulean3-cpCerulean3-FLARE-AKAR
   

   Use

   Tags

   6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Anti-Mad3 [N129A/6R]

   Plasmid

   #140075

   Purpose

   Mammalian Expression Plasmid of anti-Mad3 (Human). Derived from hybridoma N129A/6.

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-Mad3 (Human) recombinant mouse monoclonal antibody (MXD3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   dual CMV

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-GABARα6N-HA

   Plasmid

   #128111

   Purpose

   Express HA tagged GABARα6 N terminal domain

   Depositor

   Huda Zoghbi

   Article

   Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

   Insert

   Gabra6 (Gabra6 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Anti-Synaptotagmin-12 [N277/7R]

   Plasmid

   #114482

   Purpose

   Mammalian Expression Plasmid of anti-Synaptotagmin-12 (Mouse). Derived from hybridoma N277/7.

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-Synaptotagmin-12 (Mus musculus) recombinant mouse monoclonal antibody (Syt12 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   dual CMV

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJET1.2_Bod1l_Sense

   Plasmid

   #124443

   Purpose

   Plasmid for sense in situ probe in vitro transcription

   Depositor

   Yingguang Frank Chan

   Article

   Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

   Insert

   Biorientation of chromosomes in cell division 1-like (Bod1l Mouse)
   

   Use

   In situ probe

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-Hemmar-DSCP-ftz-FLP(D)

    Plasmid

    #119894

    Purpose

    FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intron

    Depositor

    Rita Sousa-Nunes

    Article

    Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

    Inserts

    FLP(D)
    DSCP-ftz (ftz Fly)
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    a mutated form of FLP, which at position 5 contai…

    Promoter

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMAL c2x Tral N

    Plasmid

    #113009

    Purpose

    For bacterial expression of MBP fusion of N terminal region of Drosophila Tral

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    N terminus of tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amino acids 1-355

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMAL c2x Tral FL-15A

    Plasmid

    #113011

    Purpose

    For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    15 PNG phosphorylation sites mutated to A

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMAL c2x Tral C

    Plasmid

    #113008

    Purpose

    For bacterial expression of MBP fusion of C terminal region of Drosophila Tral

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    C terminus of tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amino acids 356-652

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMAL c2x Tral C 15A

    Plasmid

    #113007

    Purpose

    For bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    C terminus of tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PNG phosphorylation sites mutated to A

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lrrc4c-AP-His

    Plasmid

    #71959

    Purpose

    Expresses the extracellular region of the LRRC4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Lrrc4c (Lrrc4c Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-22HT-MSI1(7-192)R53E/R61E

    Plasmid

    #60354

    Purpose

    R53E R61E double mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    R53E and R61E mutations; contains amino acids 7-1…

    Promoter

    T7

    Available since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1-PDGFRA-V5/HIS

    Plasmid

    #234754

    Purpose

    Expression of human PDGFRA receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    PDGFRA receptor tyrosine kinase, full length, wildtype (PDGFRA Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-MET-V5/HIS

    Plasmid

    #201982

    Purpose

    expression of human MET receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    MET receptor tyrosine kinase (MET Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-PDGFRA-V5/HIS

    Plasmid

    #201987

    Purpose

    expression of human PDGFRA receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    PDGFRA receptor tyrosine kinase (PDGFRA Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiMPH v2 (Neo)

    Plasmid

    #92065

    Purpose

    Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Insert

    MS2-P65-HSF1_2A_Neo (HSF1 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1a

    Available since

    June 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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