We narrowed to 5,869 results for: actin
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Plasmid#202528PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cellDepositorInsertSGMS1 (SGMS1 Human)
TagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TAA)PromoterCAG and chicken β-actin promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCA-G-intron(Neo)-T(LNL)
Plasmid#36907DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
Neo
Tags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VPS13C-WT-3xFLAG
Plasmid#248315PurposeMammalian expression of VPS13C for in vitro studiesDepositorAvailable sinceJuly 10, 2026AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_AIO-TetOn_Down-Tandem Donor
Plasmid#229783PurposeBxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)DepositorInsertLacZ; rtTA-T2A-mTagBFP2
UseSynthetic BiologyExpressionMammalianPromoterTRE and CAGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pC3.1_CAG_oROS-Gr
Plasmid#216113PurposeExpresses the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCAG_oROS-Gr
ExpressionMammalianPromoterCAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAGFLEX.(cyto).iATPSnFR2.A95A.A119L.HaloTag
Plasmid#209657PurposeExpression of iATPSnFR2, low affinityDepositorInsertiATPSnFR2.A95A.A119L.HaloTag
UseAAVMutationA95A.A119LPromoterCAGAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mScarlet-Giantin
Plasmid#196872PurposeExpresion of Giantin-fused to mScarlet. Used as Golgi markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmScarlet-Giantin (GOLGB1 Human, Synthetic)
ExpressionMammalianPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAVS1-Puro CAG PSMA nanobody T-CAR
Plasmid#171962PurposeDonor plasmid for PSMA nanobody T-CAR expression in human cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSynthetic anti-PSMA nanobody CAR
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-flex-lck-smV5-4x6T
Plasmid#196419PurposeCre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassetteDepositorInsertLck-smV5
UseAAV and Cre/Lox; Astrocyte-selectiveTagsLckExpressionMammalianPromoterCAGAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE
Plasmid#196677PurposeAAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).DepositorInsertPKIα-P2A-mRuby3
UseAAV and Cre/LoxExpressionMammalianAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG CLTX-CAR
Plasmid#157743Purposeknock CLTX-CAR into AAVS1 safe harbor locus for constitutive expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertchlorotoxin containing chimeric antigen receptor targeting glioblastoma
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FRT-Rev-3xGFP
Plasmid#191204PurposeFlpO dependent GFP expressionDepositorInsert3X GFP
UseAAVExpressionMammalianPromoterCAGAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EBFP2-P2A-EGFP
Plasmid#184938PurposeExpresses blue fluorescent protein EBFP2 and EGFP in mammalian cellsDepositorInsertEBFP2-P2A-EGFP
UseAAVExpressionMammalianPromoterCAGAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
UPB-lung (UPB2)
Plasmid#180788PurposeUnique Projection Barcode (UPB2)-Lung for Projection-seqDepositorInserthumanized ChR2-H134R (partial)
UseAAVExpressionMammalianPromoterCAGAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPyCAG-EGFP-TM-IRES-Pac
Plasmid#183603PurposeMammalian expression vector for membrane-tethered EGFP from the CAG promoter. Confers puromycin resistance.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP-TM
TagsHuman PDGFRB transmembrane domain and Mouse IgGK …ExpressionMammalianPromoterCAGAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-IRES2-p75NTR-GFP
Plasmid#182947PurposeTo express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-TerminalDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertp75NTR-mGFP
TagsHA tag and mEGFPExpressionMammalianMutationThe leader sequence is from preprotrypinPromoterCAGAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ArcLight-ST
Plasmid#182318PurposeFluorescent voltage indicator with left-shifted voltage-dependencyDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertArcLight-ST
ExpressionMammalianMutationMutation in an amino acid Q217E of ArcLight-MTPromoterCAGAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-Antares2
Plasmid#120868PurposePiggybac transposon plasmid for live animal optical imagingDepositorInsertpcDNA3-Antares2
ExpressionMammalianMutationAntares2 ampliconPromoterCAG PromoterAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-loxP-STOP-loxP-Venus
Plasmid#170520PurposePlasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )DepositorInsertCAG-loxP-STOP-loxP-Venus
ExpressionMammalianPromoterCAGAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-PE SpRY
Plasmid#179082PurposeNOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationA61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…PromoterCAGAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only