We narrowed to 7,477 results for: PAC

1. pAcUW51-gEgIL

   Plasmid

   #11622

   Depositor

   Pamela Bjorkman

   Article

   Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

   Insert

   HSV-1 glycoprotein E and HSV-1 glycoprotein I
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   Dicistronic vector containing HSV-1 gE (KOS strai…

   Promoter

   Available since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

2. Epac-S-H189

   Plasmid

   #170350

   Purpose

   mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

   Insert

   mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAcGFP1-mito

   Plasmid

   #174180

   Purpose

   Express AcGFP1 with mitochondria-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   AcGFP with mitochondria-targeting sequence
   

   Use

   Tags

   the mitochondria-targeting sequence of the cytoch…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pActin-miRFP670nano

   Plasmid

   #127428

   Purpose

   Human beta-actin labeling with near-infrared fluorescent protein miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   miRFP670nano-human beta-actin
   

   Use

   Tags

   miRFP670nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Epac-S-H21

   Plasmid

   #170335

   Purpose

   CFP(ST)_EPAC full length_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.

   Insert

   CFP(ST)_EPAC full length_Y(UST) (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEvol-pAcFRS.1.t1

   Plasmid

   #73545

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAcFRS.1.t1
   pAcFRS.1.t1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Epac-S-H30

   Plasmid

   #170337

   Purpose

   CFP(UST)_EPACdDEPCD_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.

   Insert

   CFP(UST)_EPACdDEPCD_Y(UST) (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAcGFP1-N1-RvCAHS12-GS

   Plasmid

   #192462

   Purpose

   Expression of RvCAHS12-GS_linker-AcGFP1 in mammalian cells

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

   Insert

   CAHS12
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACEBac1-CoRESTfl

   Plasmid

   #109156

   Purpose

   Encodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Inui et al Protein Sci. 2017 May;26(5):997-1011. do

   Insert

   human full-length CoREST, sequence-optimized for insect cells (RCOR1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Jan. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Epac-S-H27

    Plasmid

    #170336

    Purpose

    CFP(ST)_EPACdDEP _Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.

    Insert

    CFP(ST)_EPACdDEP _Y(UST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain

    Promoter

    CMV

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAcGFP-C2-hSphK2

    Plasmid

    #84370

    Purpose

    Expression of AcGFP-SphK2 fusion protein in mammalian cells

    Depositor

    Hugh Rosen, Anthony Don

    Article

    Don et al Biochem Biophys Res Commun. 2009 Feb 27;

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pAcBac1-haloTyrRS C6

    Plasmid

    #141174

    Purpose

    Expression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acids

    Depositor

    Ryan Mehl

    Article

    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

    Insert

    C6 HaloTyr tRNA synthetase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    L270S, Y271L, N311G, C313T

    Promoter

    CMV

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFGC5941-PacI

    Plasmid

    #44182

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAct:dCas9-GCN4

    Plasmid

    #78899

    Purpose

    Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-10XGCN4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2-mCherry_SNAP_SNAPV_PACT_Active

    Plasmid

    #173072

    Purpose

    Active SNAP tag at the centrosome

    Depositor

    Heidi Hehnly

    Article

    Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

    Insert

    mCherry-SNAP-SNAP-Varied-PACT
    

    Use

    Tags

    mCherry-SNAP-SNAP-Varied

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pACYC-GST

    Plasmid

    #62329

    Purpose

    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    Glutathione S-transferase (GST) tag and TEV prote…

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac0

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. msEGFP-PACR_pcDNA3

    Plasmid

    #55774

    Purpose

    Genetically encoded caged Ca2+

    Depositor

    Takeharu Nagai

    Article

    Fukuda et al ACS Chem Biol. 2014 May 16;9(5):1197-203

    Insert

    PACR
    

    Use

    Tags

    msEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 3, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pLKO5d.EFS.SpCas9.P2A.PAC

    Plasmid

    #58329

    Purpose

    Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    EFS (EEF1A1 Human)
    P2A-PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pActinin-LSSmOrange

    Plasmid

    #37139

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

    Insert

    LSSmOrange
    

    Use

    Tags

    human alpha-actinin

    Expression

    Mammalian

    Mutation

    A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

    Promoter

    CMV

    Available since

    June 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pL-SFFV.Reporter.RFP657.PAC

    Plasmid

    #61395

    Purpose

    Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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