We narrowed to 8,361 results for: sgrna

1. HUWE1-sgRNA-2

   Plasmid

   #86925

   Purpose

   To express sgRNA target HUWE1 gene

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   HUWE1 sgRNA2 (HUWE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pH-STEME-1-esgRNA

   Plasmid

   #138135

   Purpose

   Targeted simultaneous C-to-T and A-to-G in rice

   Depositor

   Caixia Gao

   Insert

   APOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMPTY::sgRNA2

   Plasmid

   #165459

   Purpose

   Escherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteria

   Depositor

   Inigo Lasa

   Article

   Dorado-Morales et al mBio. 2021 Feb 23;12(1). pii: mBio.03094

   Insert

   sgRNA2
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   SP01

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pH-STEME-NG-esgRNA

   Plasmid

   #138136

   Purpose

   Targeted simultaneous C-to-T and A-to-G in rice

   Depositor

   Caixia Gao

   Insert

   APOBEC3A-wtTadA-TadA7.10-nCas9-NG-UGI-NLS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6a:sgRNA(tyr)

   Plasmid

   #64250

   Purpose

   expresses sgRNA(tyr) under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Insert

   U6a:sgRNA (tyr)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiGuidPuro-hTP73_sgRNA-1

   Plasmid

   #88855

   Purpose

   CRISPR KO of Trp73

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp73
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pH-PABE-7-esgRNA

   Plasmid

   #115620

   Purpose

   Targeted A to G in rice

   Depositor

   Caixia Gao

   Article

   Li et al Genome Biol. 2018 May 29;19(1):59. doi:

   Insert

   wtTadA-TadA7.10-nCas9-3*NLS
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. sgRNA 2 GAPDH

   Plasmid

   #83809

   Purpose

   Express Sg-2 sgRNA targeting GAPDH

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   SgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPEPZ-sgRNAclone

   Plasmid

   #141090

   Purpose

   This vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.

   Depositor

   Jan-Willem Veening

   Article

   Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107

   Insert

   mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p3

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBSV2_Psyn-sgRNA500

    Plasmid

    #149613

    Purpose

    gRNA expression in B. burgdorferi, parental vector

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLH-sgRNA1

    Plasmid

    #75388

    Purpose

    sgRNA1

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. LENTICRISPR-TKT_sgRNA2

    Plasmid

    #201625

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    TKT (TKT Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LENTICRISPR-TKT_sgRNA1

    Plasmid

    #201624

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    TKT (TKT Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBbdCas9S_P0526-sgRNAmreB

    Plasmid

    #149654

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, P0526

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA

    Plasmid

    #110624

    Purpose

    A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    LP1B and hU6

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. sgRNA 1 GAPDH

    Plasmid

    #83808

    Purpose

    Express Sg-1 sgRNA targeting GAPDH

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. M-ST1-sgRNA

    Plasmid

    #48672

    Purpose

    Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hU

    Available since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgRNA with U6 promoter

    Plasmid

    #48962

    Purpose

    to drive the sgRNA expression under a U6 promoter

    Depositor

    Mario de Bono

    Article

    Chen et al Nucleic Acids Res. 2013 Sep 5.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pH-PABE-7-sgRNA

    Plasmid

    #115621

    Purpose

    Targeted A to G in rice

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    wtTadA-TadA7.10-nCas9-3*NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. TOP2A sgRNA1

    Plasmid

    #138190

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TOP2A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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