Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

163,606 results

Showing: 2981 - 3000 of 163606 results

  1. T777T

    Plasmid
    #113082
    Purpose
    Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.
    Depositor
    Tibor Vellai
    Article
    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    113082-plasmid-map-sequence-id-218958

    Enlarge

  4. mCherry-TOMM20-N-10

    Plasmid
    #55146
    Purpose
    Localization: Outer Mitochondrial Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    TOMM20 (TOMM20 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55146-plasmid-map-sequence-id-334999

    Enlarge

  6. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Ryan Mehl, Richard Cooley
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available Since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

    Enlarge

  8. pGCC4

    Plasmid
    #37058
    Depositor
    Hank Seifert
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    37058-plasmid-map-sequence-id-352924

    Enlarge

  10. pD3sE_SC12

    Plasmid
    #219720
    Purpose
    DV3 E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Article
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    DV3 sE SC12
    Use
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…
    Promoter
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219720-plasmid-map-sequence-id-433924

    Enlarge

  12. GPRC6A-DuET

    Plasmid
    #213299
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPRC6A (GPRC6A Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213299-plasmid-map-sequence-id-430843

    Enlarge

  13. pET24a-ncOGT-FL

    Plasmid
    #190821
    Purpose
    For expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E.coli
    Promoter
    T7
    Available Since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190821-plasmid-map-sequence-id-379184

    Enlarge

Showing: 2981 - 3000 of 163606 results