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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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179,521 results

Showing: 5961 - 6000 of 179521 results

  3. pCS2 GFP-GSK3-MAPK

    Plasmid
    #29689
    Purpose
    GFP reporter protein fused to three GSK3 phosphorylation sites
    Depositor
    Edward De Robertis
    Publication
    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP glycogen synthase kinase 3 recognition site primed by MAPK
    Tags
    Flag and Flag-EGFP
    Expression
    Mammalian
    Mutation
    recognition sites only, and E3 ligase site
    Promoter
    SP6
    Available since
    July 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29689-plasmid-map-sequence-id-127238

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  23. 1481 pcDNA3 flag HA 14-3-3 zeta

    Plasmid
    #9002
    Depositor
    William Sellers
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    14-3-3 zeta (YWHAZ Bovine)
    Tags
    HA and flag
    Expression
    Mammalian
    Mutation
    T226A
    Available since
    April 20, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9002-plasmid-map-sequence-id-245998

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  26. 1435 pSG5L Flag HA

    Plasmid
    #10791
    Purpose
    Mammalian expression empty backbone.
    Depositor
    William Sellers
    Type
    Empty backbone
    Tags
    Flag and HA
    Expression
    Mammalian
    Available since
    Sept. 28, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10791-plasmid-map-sequence-id-482206

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  29. AAV-hSyn-RiboL1-jGCaMP8s

    Plasmid
    #169247
    Purpose
    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.
    Depositor
    Marianne Fyhn
    Publication
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Insert
    RiboL1-jGCaMP8s
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Promoter
    hSyn
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    169247-plasmid-map-sequence-id-343245

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  32. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE

    Plasmid
    #162377
    Purpose
    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Has service
    AAV1, AAV9, and AAV Retrograde
    Insert
    jGCaMP8s
    Use
    AAV and Cre/Lox
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    S26M F286Y Q315H
    Promoter
    Synapsin
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162377-plasmid-map-sequence-id-318372

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  33. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139987-plasmid-map-sequence-id-275335

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  35. pC0079 RESCUE-S

    Plasmid
    #130662
    Purpose
    pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S
    Depositor
    Feng Zhang
    Publication
    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dRanCas13b-ADAR2dd
    Expression
    Mammalian
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    130662-plasmid-map-sequence-id-255012

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  37. pSH-EFIRES-P-AtAFB2

    Plasmid
    #129715
    Purpose
    AAVS1 safe harbor targeting vector expressing AtAFB2
    Depositor
    Elina Ikonen
    Publication
    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AFB2 (AFB2 Mustard Weed)
    Use
    CRISPR and TALEN; Human safe harbor locus (a avs1…
    Tags
    no
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    EF1a
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129715-plasmid-map-sequence-id-254833

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Showing: 5961 - 6000 of 179521 results