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We narrowed to 4,603 results for: Abo

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Showing: 4501 - 4530 of 4603 results

  5. pGTM_SUMO_PLpro_C111S

    Plasmid
    #233740
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of PLpro protease with a C111S active site mutation that abolishes protease activity. Following Sumo Protease cleavage no nonnative res
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo
    Expression
    Bacterial
    Mutation
    Cys111Ser active site mutation of the Papain-Like…
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    233740-plasmid-map-sequence-id-469323

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  6. pGTM_Sumo2-N-8XHis-Plpro-C111S

    Plasmid
    #234472
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 C111S with an activity abolishing mutation for the purification of this PLpro protease. Following Sumo Protease Cleavage an N-term 8xHis Tag is retained
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo and 8xHis
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    234472-plasmid-map-sequence-id-469247

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  19. pSynSRE-Mut-T-Luc

    Plasmid
    #60490
    Purpose
    Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988
    Depositor
    Timothy Osborne
    Article
    Smith et al J Biol Chem. 1988 Dec 5;263(34):18480-7.
    Insert
    HMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
    Use
    Luciferase
    Tags
    Luciferase reporter
    Expression
    Mammalian
    Mutation
    Four point mutations (G->C or A->C) in the …
    Promoter
    Partial HMG-CoA synthase promoter (-324/-225) fus…
    Available Since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    60490-plasmid-map-sequence-id-332535

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  20. GRM2-DuET

    Plasmid
    #213301
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM2 (GRM2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213301-plasmid-map-sequence-id-430907

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  21. GRM5-DuET

    Plasmid
    #213303
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM5 (GRM5 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213303-plasmid-map-sequence-id-431813

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  22. GRM1-DuET

    Plasmid
    #213300
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM1 (GRM1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213300-plasmid-map-sequence-id-430906

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  23. pHCAG-L2EOP

    Plasmid
    #51783
    Purpose
    The oriP/EBV nuclear antigen (EBNA)-1 elements of Epstein-Barr virus is fused in frame with the luciferase in the herpes simplex virus type-1 amplicon vector, provides prolonged transgene expression
    Depositor
    Paula Lam
    Article
    Sia et al J Gene Med. 2010 Oct;12(10):848-58. doi:
    Inserts
    Luciferase-2A-EBNA-1
    OriP
    Use
    Herpes simplex virus type-1 amplicon vector
    Promoter
    CAG
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    51783-plasmid-map-sequence-id-79421

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  25. pAAV2-CAG-3xNLS-mScarlet

    Plasmid
    #191098
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-mScarlet
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to human codon usage
    Promoter
    CMV enhancer and CAG
    Available Since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191098-plasmid-map-sequence-id-380124

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  27. K9L mutant of H3K9me3 biosensor

    Plasmid
    #120807
    Purpose
    FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Article
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Insert
    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
    Expression
    Mammalian
    Mutation
    lysine 9 is mutated to leucine 9 on histone H3
    Promoter
    CMV
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    120807-plasmid-map-sequence-id-235134

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  28. W45A mutant of H3K9me3 biosensor

    Plasmid
    #120808
    Purpose
    FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Article
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Insert
    Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
    Expression
    Mammalian
    Mutation
    Tryptophan 45 is mutated to Alanine 45 on HP1 dom…
    Promoter
    CMV
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    120808-plasmid-map-sequence-id-235136

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  29. pAAV2-CAG-3xNLS-vsfGFP-0

    Plasmid
    #191097
    Purpose
    To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-vsfGFP-0
    Use
    AAV
    Tags
    Green fluorescent protein bound to enhancer nanob…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV and CAG
    Available Since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191097-plasmid-map-sequence-id-380121

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  30. pAAV2-CAG-3xNLS-AausFP1

    Plasmid
    #191096
    Purpose
    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-AausFP1
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV enhancer and CAG
    Available Since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191096-plasmid-map-sequence-id-380123

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Showing: 4501 - 4530 of 4603 results